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The map label for this gene is cspA [H]

Identifier: 189353104

GI number: 189353104

Start: 1286803

End: 1287006

Strand: Reverse

Name: cspA [H]

Synonym: BMULJ_04340

Alternate gene names: 189353104

Gene position: 1287006-1286803 (Counterclockwise)

Preceding gene: 189353105

Following gene: 189353103

Centisome position: 52.04

GC content: 55.88

Gene sequence:

>204_bases
ATGGATACCGGTACGGTCAAGTGGTTCAACGAAACGAAGGGTTTCGGTTTCATCAGTCCCGATAACGGCGGCGACGATCT
GTTCGCGCACTTTTCGGAAATTCGCGGTACGGGCTTCAAGACGCTCACCGACAACCAGAAGGTCAGCTACGAAGTGAAGC
GCGGCCCGAAAGGTCTGCAGGCGTCGAACATCACGCCGCTGTAA

Upstream 100 bases:

>100_bases
TCTCCGCGTCGCACGCGGCAGTCGTTGGTCTCTCCCTCTTTGATTCTCTTTCGCGCCCGTCGCGGGCGTGCGTTCACTCA
TTCGCATCAAGGAAGCATGC

Downstream 100 bases:

>100_bases
GACCGCGCGCGGGCGCGGCCATGCGGCCCGCCCGCATGGCATGCCGGCGTCGCGCAGGCCGCGTTGCAGCTTGCGTTCAT
TCACGTTGTCTGGAGGAATC

Product: CspA family cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
MDTGTVKWFNETKGFGFISPDNGGDDLFAHFSEIRGTGFKTLTDNQKVSYEVKRGPKGLQASNITPL

Sequences:

>Translated_67_residues
MDTGTVKWFNETKGFGFISPDNGGDDLFAHFSEIRGTGFKTLTDNQKVSYEVKRGPKGLQASNITPL
>Mature_67_residues
MDTGTVKWFNETKGFGFISPDNGGDDLFAHFSEIRGTGFKTLTDNQKVSYEVKRGPKGLQASNITPL

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=55.2238805970149, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=56.25, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=54.6875, Blast_Score=82, Evalue=8e-18,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=55.3846153846154, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=50.7692307692308, Blast_Score=79, Evalue=6e-17,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=53.8461538461538, Blast_Score=78, Evalue=9e-17,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=51.6129032258064, Blast_Score=72, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24658520, Length=62, Percent_Identity=40.3225806451613, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7368; Mature: 7368

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGTVKWFNETKGFGFISPDNGGDDLFAHFSEIRGTGFKTLTDNQKVSYEVKRGPKGLQ
CCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCCCC
ASNITPL
CCCCCCC
>Mature Secondary Structure
MDTGTVKWFNETKGFGFISPDNGGDDLFAHFSEIRGTGFKTLTDNQKVSYEVKRGPKGLQ
CCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCCCC
ASNITPL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10483731 [H]