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The map label for this gene is otsA [H]

Identifier: 189353086

GI number: 189353086

Start: 1265287

End: 1266687

Strand: Direct

Name: otsA [H]

Synonym: BMULJ_04322

Alternate gene names: 189353086

Gene position: 1265287-1266687 (Clockwise)

Preceding gene: 189353085

Following gene: 189353089

Centisome position: 51.16

GC content: 66.95

Gene sequence:

>1401_bases
GTGAGCCGTCTCGTCGTCGTATCGAATCGCATTGCAGATCCCCGTAAAGCCGCGGCCGGCGGTCTCGCCGTCGCCGTGAA
AGACAGCCTGCAGGAAACGGGCGGCGTCTGGTTCGGCTGGAGCGGCAAGCTACGCAGCGCCGACGCACAGCCCGCACACG
GCGACGACGTGCAGATCCAGAACGTCGGCGGCATTCAGCTCGCGACGATCGATCTCGATCCACAGGATTACGACGCGTAC
TACCTCGGCTACTCGAACAACGTGCTGTGGCCGGTGTTCCATTACCGGCTCGATCTCGCGCAGTTCGACCGCCGTTTCGC
CGACGGCTACCGGCGCGTGAACCAGCTGTTCGCGCGTCGGCTGCGCACGCTGCTGCGGCCCGACGACGTCGTGTGGGTTC
ACGACTATCAGCTGATTCCGCTCGCGGCCGAATTGCGCGCGATGGGCTGCACGAATCCGATCGGCTTCTTCCTGCACATT
CCGGTGCCGCCGCCGCCGATCATGGCCGCGATTCCGGAGCACGAATGGCTGATGCGCTCGCTGTTCGCGTACGACCTCGT
CGGCTTCCAGACCGAGGCCGACCTGCTGCACTTCGAGCATTACGTCGAAGCGGAAGCCGGCGCCGCGCGCCTCGCGGACG
GCCGACTGCGCGCCTTCGGCCGCACGCTGTCGGCCGGCGCGTTTCCGATCGGCATCAACGTCGACGAATTCGCGGCGCTC
GCCGACGATCGCGACGGCATCGACATGTTCGAGCGGATGCGCGACGAGTATTCGCGCCGGCAACTGCTCGTCGGCGTCGA
CCGGCTCGACTACACGAAGGGGTTGCCGCAGCGCGTGCATGCGTTCCGGCAATTGCTCGAACAGTATCCTGAGAACCGCA
ATCGCGCGACGCTGATCCAGATCGCCGCACCGAGCCGCGAGGATCTCGGCGCATACGACGATCTGCGCCGCGAGATGGAC
AGTCTCTGCGGTGCGATCAACGGCGACTACGGCGAGCTCGAATGGATGCCGATGCGCTACATCCATCGCACGGTCGCGCG
CAAGCGGCTGCCGGGACTCTATCGCGCGAGCCGCGTCGCGCTCGTGACGCCGCTGCGCGACGGGATGAATCTCGTCGCGA
AGGAATTCCTCGCCGCGCAGGACGAAGCCGATCCCGGCGTACTCGTGCTGTCGCGCTTCGCCGGTGCGGCCGAGCAGCTG
AAGGAAGCGCTGCTCGTGAACCCGTACGACACGCAAGGCACCGCGCAGGCGATCCAGCGTGCGCTCGCGATGCCGCTCGA
GGAACGCCGACAGCGTCATGCGGCGCTGATGGCGACGCTGCGGCGCACCGACGTGCAGTGGTGGCGCAAGCGCTTTCTCG
AAGCGCTCGCGGAAGCGGCCGAAGTCGCCGACGCGACGTAG

Upstream 100 bases:

>100_bases
TCCGATCGTACTGCGGTCCCGTCGCGCGCGTCGCGACCTTGGCGGCATCCGCTGCCGCCCGGCCGCACGACCTTTCCCTC
AACAACCGTCAGGAGAGCTG

Downstream 100 bases:

>100_bases
TGCGCCGCGCGTCACGCGCCTGCTTCGTTCGGCTCGTCTGCTTCGTCCGCTTTCAATCGCTCGCCGCGTCGCCGCGTCGC
CGCGTCGCGGCTTTACGTCC

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSRLVVVSNRIADPRKAAAGGLAVAVKDSLQETGGVWFGWSGKLRSADAQPAHGDDVQIQNVGGIQLATIDLDPQDYDAY
YLGYSNNVLWPVFHYRLDLAQFDRRFADGYRRVNQLFARRLRTLLRPDDVVWVHDYQLIPLAAELRAMGCTNPIGFFLHI
PVPPPPIMAAIPEHEWLMRSLFAYDLVGFQTEADLLHFEHYVEAEAGAARLADGRLRAFGRTLSAGAFPIGINVDEFAAL
ADDRDGIDMFERMRDEYSRRQLLVGVDRLDYTKGLPQRVHAFRQLLEQYPENRNRATLIQIAAPSREDLGAYDDLRREMD
SLCGAINGDYGELEWMPMRYIHRTVARKRLPGLYRASRVALVTPLRDGMNLVAKEFLAAQDEADPGVLVLSRFAGAAEQL
KEALLVNPYDTQGTAQAIQRALAMPLEERRQRHAALMATLRRTDVQWWRKRFLEALAEAAEVADAT

Sequences:

>Translated_466_residues
MSRLVVVSNRIADPRKAAAGGLAVAVKDSLQETGGVWFGWSGKLRSADAQPAHGDDVQIQNVGGIQLATIDLDPQDYDAY
YLGYSNNVLWPVFHYRLDLAQFDRRFADGYRRVNQLFARRLRTLLRPDDVVWVHDYQLIPLAAELRAMGCTNPIGFFLHI
PVPPPPIMAAIPEHEWLMRSLFAYDLVGFQTEADLLHFEHYVEAEAGAARLADGRLRAFGRTLSAGAFPIGINVDEFAAL
ADDRDGIDMFERMRDEYSRRQLLVGVDRLDYTKGLPQRVHAFRQLLEQYPENRNRATLIQIAAPSREDLGAYDDLRREMD
SLCGAINGDYGELEWMPMRYIHRTVARKRLPGLYRASRVALVTPLRDGMNLVAKEFLAAQDEADPGVLVLSRFAGAAEQL
KEALLVNPYDTQGTAQAIQRALAMPLEERRQRHAALMATLRRTDVQWWRKRFLEALAEAAEVADAT
>Mature_465_residues
SRLVVVSNRIADPRKAAAGGLAVAVKDSLQETGGVWFGWSGKLRSADAQPAHGDDVQIQNVGGIQLATIDLDPQDYDAYY
LGYSNNVLWPVFHYRLDLAQFDRRFADGYRRVNQLFARRLRTLLRPDDVVWVHDYQLIPLAAELRAMGCTNPIGFFLHIP
VPPPPIMAAIPEHEWLMRSLFAYDLVGFQTEADLLHFEHYVEAEAGAARLADGRLRAFGRTLSAGAFPIGINVDEFAALA
DDRDGIDMFERMRDEYSRRQLLVGVDRLDYTKGLPQRVHAFRQLLEQYPENRNRATLIQIAAPSREDLGAYDDLRREMDS
LCGAINGDYGELEWMPMRYIHRTVARKRLPGLYRASRVALVTPLRDGMNLVAKEFLAAQDEADPGVLVLSRFAGAAEQLK
EALLVNPYDTQGTAQAIQRALAMPLEERRQRHAALMATLRRTDVQWWRKRFLEALAEAAEVADAT

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=460, Percent_Identity=47.1739130434783, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI115533560, Length=369, Percent_Identity=31.9783197831978, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI115533558, Length=369, Percent_Identity=31.9783197831978, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71987012, Length=433, Percent_Identity=28.6374133949192, Blast_Score=167, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=32.9787234042553, Blast_Score=279, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6320279, Length=401, Percent_Identity=26.9326683291771, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323537, Length=392, Percent_Identity=29.0816326530612, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6323917, Length=412, Percent_Identity=26.9417475728155, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19920676, Length=489, Percent_Identity=38.4458077709611, Blast_Score=307, Evalue=8e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52589; Mature: 52457

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVVSNRIADPRKAAAGGLAVAVKDSLQETGGVWFGWSGKLRSADAQPAHGDDVQIQ
CCCEEEECCCCCCCHHHHCCCEEEEEHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEE
NVGGIQLATIDLDPQDYDAYYLGYSNNVLWPVFHYRLDLAQFDRRFADGYRRVNQLFARR
ECCCEEEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRTLLRPDDVVWVHDYQLIPLAAELRAMGCTNPIGFFLHIPVPPPPIMAAIPEHEWLMRS
HHHHHCCCCEEEEECCEEEHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCHHHHHHH
LFAYDLVGFQTEADLLHFEHYVEAEAGAARLADGRLRAFGRTLSAGAFPIGINVDEFAAL
HHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCEEECCCHHHHHHH
ADDRDGIDMFERMRDEYSRRQLLVGVDRLDYTKGLPQRVHAFRQLLEQYPENRNRATLIQ
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IAAPSREDLGAYDDLRREMDSLCGAINGDYGELEWMPMRYIHRTVARKRLPGLYRASRVA
EECCCCHHCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHCCEE
LVTPLRDGMNLVAKEFLAAQDEADPGVLVLSRFAGAAEQLKEALLVNPYDTQGTAQAIQR
EEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHH
ALAMPLEERRQRHAALMATLRRTDVQWWRKRFLEALAEAAEVADAT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRLVVVSNRIADPRKAAAGGLAVAVKDSLQETGGVWFGWSGKLRSADAQPAHGDDVQIQ
CCEEEECCCCCCCHHHHCCCEEEEEHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEE
NVGGIQLATIDLDPQDYDAYYLGYSNNVLWPVFHYRLDLAQFDRRFADGYRRVNQLFARR
ECCCEEEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRTLLRPDDVVWVHDYQLIPLAAELRAMGCTNPIGFFLHIPVPPPPIMAAIPEHEWLMRS
HHHHHCCCCEEEEECCEEEHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCHHHHHHH
LFAYDLVGFQTEADLLHFEHYVEAEAGAARLADGRLRAFGRTLSAGAFPIGINVDEFAAL
HHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCEEECCCHHHHHHH
ADDRDGIDMFERMRDEYSRRQLLVGVDRLDYTKGLPQRVHAFRQLLEQYPENRNRATLIQ
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IAAPSREDLGAYDDLRREMDSLCGAINGDYGELEWMPMRYIHRTVARKRLPGLYRASRVA
EECCCCHHCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHCCEE
LVTPLRDGMNLVAKEFLAAQDEADPGVLVLSRFAGAAEQLKEALLVNPYDTQGTAQAIQR
EEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHH
ALAMPLEERRQRHAALMATLRRTDVQWWRKRFLEALAEAAEVADAT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]