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The map label for this gene is livK [C]

Identifier: 189353011

GI number: 189353011

Start: 1174402

End: 1175523

Strand: Direct

Name: livK [C]

Synonym: BMULJ_04245

Alternate gene names: 189353011

Gene position: 1174402-1175523 (Clockwise)

Preceding gene: 189353010

Following gene: 189353012

Centisome position: 47.49

GC content: 66.58

Gene sequence:

>1122_bases
ATGCGATACCTCGTTACCGTGCTGTCGGTCGCCGTCGCGGCCGGCACGCTCGCGTCGGCTCATGCGCAGGAAGTCGTGAC
GATCGGCCACTCGGCGCCGCTCACGGGCCCGCAGGCCGCGAACGGCAAGGACAACGAAAACGGCGCGCGGCTCGCGGTCG
ACGAACTGAACCGGGCCGGCGTGAAGGTCGCGGGCAAGACGGTCACGTTCCGGCTGCTGTCGGAGGACGATCAGGCCGAT
CCGAAGGTTGGCGTGCAGGTCGCACAGAACCTCGTCGACAAGGGCGTCGTCGCGGTGCTTGGCCCATACAACTCGGGCGT
CGCGATTCCGGCGTCGCGCGTGTATGCGAACGCCGGCGTGCCGATCCTGCCGGTCGCGTCGAATCCGGCGCTCACGCGGC
AGGGCTTCAAGAACATTTTCCGGATCGGTGCGAGCGACGAGCAGCTCGGCGGCACGATGGCCGCGTTCGCCGCGAAGACG
CTGAACGCGAAGACGGCCGCGATCATCGACGATCGAACCGCATACGGGCAAGGGATCGCCGAGCAGTTCGCGAAAGTCGC
GAAGGCCAACGGCATCCGGATCGTCGACCAGGAATACACGAATTCGTCGGCGACCGATTTCCTCGGGATCCTCACCAAGA
TCAAGGCCGGCAATCCGGACGTGATCTTCTTCGGCGGCTATGCGGCGCAAGGCGCACCGATGGCCAAGCAGATGAAGCAG
CGCGGGCTGCGCGCGAAGCTGCTCGGCGGCGACGGCATCTGCTCGGCCGACATGGGCAAGGTAGCGGGCGAGGCGGCGTC
GATCGTCTACTGCGCGCAAGGCGGCGTCGCGCTCGACAAGACGCCGGCCGGCCGCGCATTCCTGAAGAAGTATCACGACA
CCTATCACGCCGACACGCAGGTGTACGCCGTGAACTATTACGACGGCGTGAAGCTGCTCGCCGATGCGATGGCCAAGGCC
GGCACGACCACCGACAAGGCGAAGCTGATCGCGCAGCTCGCGAAGTCGAACTATGCGGGCGTCGCGGGCACCTATTCGTT
CGACGCGAACGGCGACCTGCAGGGCGCGCCGACGACCGTCTACGTGATCCGCAACGGCTTGCCCGAGCCCTACGCACGAT
AG

Upstream 100 bases:

>100_bases
TGACGCCGGAACCAGATTGCCGCCGATTATTTTGAAATTGCTTGCGGGGCGACCACGCGTCGTCTACATTGGTTGCGATC
CAACCAGTCGGGAGCGCGTC

Downstream 100 bases:

>100_bases
CCGTACGCCCGTCGCCGGCGGCGCACCGCGCCGTCGTCGTTTCGCCTTCACGTTCCCGGATCGATCATGAAGATCTCCCG
TTCCCTGTCCACCGTCGAAG

Product: ABC-type branched-chain amino acid transport system periplasmic component

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRYLVTVLSVAVAAGTLASAHAQEVVTIGHSAPLTGPQAANGKDNENGARLAVDELNRAGVKVAGKTVTFRLLSEDDQAD
PKVGVQVAQNLVDKGVVAVLGPYNSGVAIPASRVYANAGVPILPVASNPALTRQGFKNIFRIGASDEQLGGTMAAFAAKT
LNAKTAAIIDDRTAYGQGIAEQFAKVAKANGIRIVDQEYTNSSATDFLGILTKIKAGNPDVIFFGGYAAQGAPMAKQMKQ
RGLRAKLLGGDGICSADMGKVAGEAASIVYCAQGGVALDKTPAGRAFLKKYHDTYHADTQVYAVNYYDGVKLLADAMAKA
GTTTDKAKLIAQLAKSNYAGVAGTYSFDANGDLQGAPTTVYVIRNGLPEPYAR

Sequences:

>Translated_373_residues
MRYLVTVLSVAVAAGTLASAHAQEVVTIGHSAPLTGPQAANGKDNENGARLAVDELNRAGVKVAGKTVTFRLLSEDDQAD
PKVGVQVAQNLVDKGVVAVLGPYNSGVAIPASRVYANAGVPILPVASNPALTRQGFKNIFRIGASDEQLGGTMAAFAAKT
LNAKTAAIIDDRTAYGQGIAEQFAKVAKANGIRIVDQEYTNSSATDFLGILTKIKAGNPDVIFFGGYAAQGAPMAKQMKQ
RGLRAKLLGGDGICSADMGKVAGEAASIVYCAQGGVALDKTPAGRAFLKKYHDTYHADTQVYAVNYYDGVKLLADAMAKA
GTTTDKAKLIAQLAKSNYAGVAGTYSFDANGDLQGAPTTVYVIRNGLPEPYAR
>Mature_373_residues
MRYLVTVLSVAVAAGTLASAHAQEVVTIGHSAPLTGPQAANGKDNENGARLAVDELNRAGVKVAGKTVTFRLLSEDDQAD
PKVGVQVAQNLVDKGVVAVLGPYNSGVAIPASRVYANAGVPILPVASNPALTRQGFKNIFRIGASDEQLGGTMAAFAAKT
LNAKTAAIIDDRTAYGQGIAEQFAKVAKANGIRIVDQEYTNSSATDFLGILTKIKAGNPDVIFFGGYAAQGAPMAKQMKQ
RGLRAKLLGGDGICSADMGKVAGEAASIVYCAQGGVALDKTPAGRAFLKKYHDTYHADTQVYAVNYYDGVKLLADAMAKA
GTTTDKAKLIAQLAKSNYAGVAGTYSFDANGDLQGAPTTVYVIRNGLPEPYAR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=353, Percent_Identity=28.8951841359773, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI48994941, Length=354, Percent_Identity=25.7062146892655, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 38703; Mature: 38703

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLVTVLSVAVAAGTLASAHAQEVVTIGHSAPLTGPQAANGKDNENGARLAVDELNRAG
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCC
VKVAGKTVTFRLLSEDDQADPKVGVQVAQNLVDKGVVAVLGPYNSGVAIPASRVYANAGV
EEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHEECCCC
PILPVASNPALTRQGFKNIFRIGASDEQLGGTMAAFAAKTLNAKTAAIIDDRTAYGQGIA
EEEEECCCCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCCCEEEEECCCHHHCCHHH
EQFAKVAKANGIRIVDQEYTNSSATDFLGILTKIKAGNPDVIFFGGYAAQGAPMAKQMKQ
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
RGLRAKLLGGDGICSADMGKVAGEAASIVYCAQGGVALDKTPAGRAFLKKYHDTYHADTQ
CCCEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCE
VYAVNYYDGVKLLADAMAKAGTTTDKAKLIAQLAKSNYAGVAGTYSFDANGDLQGAPTTV
EEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEE
YVIRNGLPEPYAR
EEEECCCCCCCCC
>Mature Secondary Structure
MRYLVTVLSVAVAAGTLASAHAQEVVTIGHSAPLTGPQAANGKDNENGARLAVDELNRAG
CHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCC
VKVAGKTVTFRLLSEDDQADPKVGVQVAQNLVDKGVVAVLGPYNSGVAIPASRVYANAGV
EEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHEECCCC
PILPVASNPALTRQGFKNIFRIGASDEQLGGTMAAFAAKTLNAKTAAIIDDRTAYGQGIA
EEEEECCCCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCCCEEEEECCCHHHCCHHH
EQFAKVAKANGIRIVDQEYTNSSATDFLGILTKIKAGNPDVIFFGGYAAQGAPMAKQMKQ
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHH
RGLRAKLLGGDGICSADMGKVAGEAASIVYCAQGGVALDKTPAGRAFLKKYHDTYHADTQ
CCCEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCE
VYAVNYYDGVKLLADAMAKAGTTTDKAKLIAQLAKSNYAGVAGTYSFDANGDLQGAPTTV
EEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEE
YVIRNGLPEPYAR
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]