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The map label for this gene is yafC [H]

Identifier: 189352987

GI number: 189352987

Start: 1148890

End: 1149837

Strand: Direct

Name: yafC [H]

Synonym: BMULJ_04221

Alternate gene names: 189352987

Gene position: 1148890-1149837 (Clockwise)

Preceding gene: 189352984

Following gene: 189352990

Centisome position: 46.45

GC content: 67.72

Gene sequence:

>948_bases
ATGGATACGCTTCAAAACATGCGGGTGTTTACGCGCGTGGTCGAGACGGGCAGTTTCACCGCGGCCGCGCAATCGCTGAA
TTCGACGACGGGCGCGATGTCGCGCGCGGTGTCGGAGCTCGAGGCGCGGCTGCGCACCCGTCTGATGAATCGCTCGACGC
GTCGTCTCGCGCTCACGTCGGCCGGCGAGAACTATCTGCGGCGCTGCCGACAGATCCTCGCGGACGTCGATCGTGCGGAA
GAAGAGGCCAGCTGCGCGCACGAGCGGCCGGCGGGCGTGCTGCGGATGCACAGCTTTGCGAGCGTCGGGCATCACTATGT
GCTGCCGGCGCTCACGCGCTATCACGCGCAGTATCCGGAGGTGTCGATCGAGCTGTCGCTGTCGCAGCGGATGCCGGACC
TGTTCGACGGGACGAGCGACATGGCGGTCGTCACGGCGTCGTCGCTGCCCGATTCGGAACTGGTGTCGCACCTGCTCGGC
TCGACGTTCAGCATTCTCTGCGCGTCGCCCGACTATGTGCGGCGGCACGGCGCGCCGGCACGCCCGCAGGAACTCGCCGC
GCATGCCTGCCTGACGCTGCGCACGCCCGCGTTCCCGACGCACGAGTGGGTGCTCGAAGGGCCGGACGGCGTCGAGCAGA
TCCACGTGAGCGGGCCGGTGCAGACGAATACGGCCGAATCGCTCGCGCTCGCGATTCGCGACGGGATCGGCATCGGCATG
CTGCCGCTCTATGCCGCGATCGATGCGCTCAGCGACGGCACGCTCGTGCGCGTGCTGCCCGAGCACATCCTGCAGAAGAT
GAACGTGTATGCGCTCTATCCGTCGCGCCGCTACATCGACGCGAAGGTGCGCACGTGGGTCGAACTGCTGCGCGCACAGG
TGCCCGCGATGATCGAGCGCGACGTCGATACGCTGAACGCGATCGGTCGCGCATCGCAGGCCGCATGA

Upstream 100 bases:

>100_bases
GCATTAACGCAGCTGCGGGGAAACGATAGTTGCAGCGCAAGTAACAATCTGCTGCACAGGCTGGAGGAAAATGGCGGCTC
CGCTCTACGTGTGAACAGGA

Downstream 100 bases:

>100_bases
CGGCGATTGCGACGCGCTGCGGCACGCGGCGCGCAATGGAGCGGCAACGTTGAAGCGCCGCGCGCCGCAGGTTTCGTCGC
GGCGCGGCCGATGCCGCGTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MDTLQNMRVFTRVVETGSFTAAAQSLNSTTGAMSRAVSELEARLRTRLMNRSTRRLALTSAGENYLRRCRQILADVDRAE
EEASCAHERPAGVLRMHSFASVGHHYVLPALTRYHAQYPEVSIELSLSQRMPDLFDGTSDMAVVTASSLPDSELVSHLLG
STFSILCASPDYVRRHGAPARPQELAAHACLTLRTPAFPTHEWVLEGPDGVEQIHVSGPVQTNTAESLALAIRDGIGIGM
LPLYAAIDALSDGTLVRVLPEHILQKMNVYALYPSRRYIDAKVRTWVELLRAQVPAMIERDVDTLNAIGRASQAA

Sequences:

>Translated_315_residues
MDTLQNMRVFTRVVETGSFTAAAQSLNSTTGAMSRAVSELEARLRTRLMNRSTRRLALTSAGENYLRRCRQILADVDRAE
EEASCAHERPAGVLRMHSFASVGHHYVLPALTRYHAQYPEVSIELSLSQRMPDLFDGTSDMAVVTASSLPDSELVSHLLG
STFSILCASPDYVRRHGAPARPQELAAHACLTLRTPAFPTHEWVLEGPDGVEQIHVSGPVQTNTAESLALAIRDGIGIGM
LPLYAAIDALSDGTLVRVLPEHILQKMNVYALYPSRRYIDAKVRTWVELLRAQVPAMIERDVDTLNAIGRASQAA
>Mature_315_residues
MDTLQNMRVFTRVVETGSFTAAAQSLNSTTGAMSRAVSELEARLRTRLMNRSTRRLALTSAGENYLRRCRQILADVDRAE
EEASCAHERPAGVLRMHSFASVGHHYVLPALTRYHAQYPEVSIELSLSQRMPDLFDGTSDMAVVTASSLPDSELVSHLLG
STFSILCASPDYVRRHGAPARPQELAAHACLTLRTPAFPTHEWVLEGPDGVEQIHVSGPVQTNTAESLALAIRDGIGIGM
LPLYAAIDALSDGTLVRVLPEHILQKMNVYALYPSRRYIDAKVRTWVELLRAQVPAMIERDVDTLNAIGRASQAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=33.6734693877551, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=32.9861111111111, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=33.5616438356164, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=30.9027777777778, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=29.4520547945205, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=26.4604810996564, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=26.530612244898, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787879, Length=154, Percent_Identity=33.1168831168831, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787601, Length=277, Percent_Identity=25.9927797833935, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786448, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788481, Length=263, Percent_Identity=28.5171102661597, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789173, Length=269, Percent_Identity=24.5353159851301, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787806, Length=126, Percent_Identity=32.5396825396825, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=22.8956228956229, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=161, Percent_Identity=24.8447204968944, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34586; Mature: 34586

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLQNMRVFTRVVETGSFTAAAQSLNSTTGAMSRAVSELEARLRTRLMNRSTRRLALTS
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHH
AGENYLRRCRQILADVDRAEEEASCAHERPAGVLRMHSFASVGHHYVLPALTRYHAQYPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
VSIELSLSQRMPDLFDGTSDMAVVTASSLPDSELVSHLLGSTFSILCASPDYVRRHGAPA
EEEEEEHHHCCCHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCC
RPQELAAHACLTLRTPAFPTHEWVLEGPDGVEQIHVSGPVQTNTAESLALAIRDGIGIGM
CHHHHHHHHHHEECCCCCCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHH
LPLYAAIDALSDGTLVRVLPEHILQKMNVYALYPSRRYIDAKVRTWVELLRAQVPAMIER
HHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
DVDTLNAIGRASQAA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTLQNMRVFTRVVETGSFTAAAQSLNSTTGAMSRAVSELEARLRTRLMNRSTRRLALTS
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEHH
AGENYLRRCRQILADVDRAEEEASCAHERPAGVLRMHSFASVGHHYVLPALTRYHAQYPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
VSIELSLSQRMPDLFDGTSDMAVVTASSLPDSELVSHLLGSTFSILCASPDYVRRHGAPA
EEEEEEHHHCCCHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCC
RPQELAAHACLTLRTPAFPTHEWVLEGPDGVEQIHVSGPVQTNTAESLALAIRDGIGIGM
CHHHHHHHHHHEECCCCCCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHH
LPLYAAIDALSDGTLVRVLPEHILQKMNVYALYPSRRYIDAKVRTWVELLRAQVPAMIER
HHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
DVDTLNAIGRASQAA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]