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The map label for this gene is menE [H]

Identifier: 189352962

GI number: 189352962

Start: 1120370

End: 1122022

Strand: Direct

Name: menE [H]

Synonym: BMULJ_04196

Alternate gene names: 189352962

Gene position: 1120370-1122022 (Clockwise)

Preceding gene: 189352954

Following gene: 189352966

Centisome position: 45.3

GC content: 68.12

Gene sequence:

>1653_bases
ATGACGCAGATGTTCGAGGCCGGGCTCGGCCGCCGCGAGGCGAACTACGTACCGCTGACGCCGATCGATTTTCTGGTGCG
CTCGGCCGAAGTCTATGGCGATCGCGTCGCGATCGTGCACGGCGACGTGCGGCGCACCTGGGCCGAAACCTACGCCCGCG
CACGGCGCCTCGCGAGCGCGCTCGCGGCAGCGGGCGTCGGGCGCGGCGAGACGGTCGCCGCGCTGCTGCCGAACATTCCG
GCGATGGTCGAAGCGCACTTCGGCGTGCCGATGGCGGGCGCGGTGCTCAATACGATCAACACGCGGCTCGACGCCGCGTC
GGTGCTGTTCATGCTGCGTCACGGCGAAGCGAAGGTGCTGATCGTCGATACCGAATATGCGGACATCGCCCAGCGCGCCG
CGCAGGAATTGCCGGGGCTGAAGCTCGTCAGCGTCGCGGACGCGATGCCGGCCGACCCGGCGCGCTTCGCGGGCGCGATC
GACTACGAGGCGTTCGTCGCAAGCGGCGACCCCGACTACGCATGGCATCCGCCGGCCGACGAGTGGGAAGCGATCGCGCT
GAACTACACGTCGGGTACGACCGGCGATCCGAAGGGCGTCGTCTATCACCACCGCGGCGCGTATCTCGCGGCGATCAGCA
ACATCCTCGAATGGGACATGCCGAAGCACGCGGTGTATCTGTGGACGCTGCCGATGTTCCACTGCAACGGCTGGTGCTTT
CCGTGGGCGGTCGCCGCGCGCGCGGGCGTGAACGTCTGCCTGCGCAAGTTCGACGCGAAGACGGTGTTCGACCTGATTCG
CCGCGAGCGCGTCACGCATTACTGCGGCGCGCCGATCGTGCAGAGCGCGATCGCCAACGCGCCGGCCGAGCTGCGCGAGG
GCATCGACCATACGGTGCACGCGATGGTCGCCGGCGCGGCGCCCGCGCCTGCCGTGATCGCGAAGATGAAGGAGATCGGC
TTCGATCTGCTGCATGTGTACGGACTGACCGAGGTGTACGGCCCGGCCACCGTGTGCGCGAAGCAGGGGCACTGGGACGC
GCTGTCCGACGACGAGCGCGCGCGGCTCAATGCGCGGCAGGGCGTGCGCTATCACCTCGAAGCGGGCGCGACGGTGCTCG
ATCCCGACACGATGGCGCCGGTGCCGGCCGACGGCGAGACGCTCGGCGAAATCATGTTCCGCGGCAACATCTGCATGAAG
GGCTACCTGAAGAACGAGAAGGCGACCGACGAAGCGTTCCACGGCGGCTGGTTCCATACCGGCGATCTGGGCGTGCTGAC
GCCGGACGGCTATATCCGCATCAAGGATCGCCGCAAGGACATCATCATCTCGGGCGGCGAGAACATCTCGAGCATCGAGG
TCGAGGACGCGCTGTACCGGCATCCGGCCGTCGCGGTCGCGGCGGTCGTCGCGATGCCGGACCCGAAGTGGGGCGAGGTG
CCGTGCGCGTTCGTCGAGCTGCGCGACGGCGCGAGCGCGACCGAGGAGGAGATTGTCGCGCACTGCCGTCAGCTGCTCGC
CGGCTTCAAGGTGCCGAAGGCCGTACGATTCGGCGAACTGCCGAAGACCTCGACCGGCAAGATCCAGAAGTTTCAGTTGC
GCCATGCGGTCGGTTCGGCCGGCGCGATCGATCTGGCCGGCGACAAGAAATAG

Upstream 100 bases:

>100_bases
ACAGACGGCTCACAATAGCGCCCGTTAGGCTGTCGGCAATTGAACCACATCAAAACGATCCGGGGGGCAGGCCGTTCGGA
TCGACACGGGAGACAGGATC

Downstream 100 bases:

>100_bases
GCAATAGGCGCGCGGCCGTGCGCGGCCGCGGCGCCGCTCACGCGTCCTGCGCGAGGCGGAACACGCTGACCGCGTCGGAC
AGCTGCGCGGCCTGGTCGCG

Product: acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTQMFEAGLGRREANYVPLTPIDFLVRSAEVYGDRVAIVHGDVRRTWAETYARARRLASALAAAGVGRGETVAALLPNIP
AMVEAHFGVPMAGAVLNTINTRLDAASVLFMLRHGEAKVLIVDTEYADIAQRAAQELPGLKLVSVADAMPADPARFAGAI
DYEAFVASGDPDYAWHPPADEWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNILEWDMPKHAVYLWTLPMFHCNGWCF
PWAVAARAGVNVCLRKFDAKTVFDLIRRERVTHYCGAPIVQSAIANAPAELREGIDHTVHAMVAGAAPAPAVIAKMKEIG
FDLLHVYGLTEVYGPATVCAKQGHWDALSDDERARLNARQGVRYHLEAGATVLDPDTMAPVPADGETLGEIMFRGNICMK
GYLKNEKATDEAFHGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVAVAAVVAMPDPKWGEV
PCAFVELRDGASATEEEIVAHCRQLLAGFKVPKAVRFGELPKTSTGKIQKFQLRHAVGSAGAIDLAGDKK

Sequences:

>Translated_550_residues
MTQMFEAGLGRREANYVPLTPIDFLVRSAEVYGDRVAIVHGDVRRTWAETYARARRLASALAAAGVGRGETVAALLPNIP
AMVEAHFGVPMAGAVLNTINTRLDAASVLFMLRHGEAKVLIVDTEYADIAQRAAQELPGLKLVSVADAMPADPARFAGAI
DYEAFVASGDPDYAWHPPADEWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNILEWDMPKHAVYLWTLPMFHCNGWCF
PWAVAARAGVNVCLRKFDAKTVFDLIRRERVTHYCGAPIVQSAIANAPAELREGIDHTVHAMVAGAAPAPAVIAKMKEIG
FDLLHVYGLTEVYGPATVCAKQGHWDALSDDERARLNARQGVRYHLEAGATVLDPDTMAPVPADGETLGEIMFRGNICMK
GYLKNEKATDEAFHGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVAVAAVVAMPDPKWGEV
PCAFVELRDGASATEEEIVAHCRQLLAGFKVPKAVRFGELPKTSTGKIQKFQLRHAVGSAGAIDLAGDKK
>Mature_549_residues
TQMFEAGLGRREANYVPLTPIDFLVRSAEVYGDRVAIVHGDVRRTWAETYARARRLASALAAAGVGRGETVAALLPNIPA
MVEAHFGVPMAGAVLNTINTRLDAASVLFMLRHGEAKVLIVDTEYADIAQRAAQELPGLKLVSVADAMPADPARFAGAID
YEAFVASGDPDYAWHPPADEWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNILEWDMPKHAVYLWTLPMFHCNGWCFP
WAVAARAGVNVCLRKFDAKTVFDLIRRERVTHYCGAPIVQSAIANAPAELREGIDHTVHAMVAGAAPAPAVIAKMKEIGF
DLLHVYGLTEVYGPATVCAKQGHWDALSDDERARLNARQGVRYHLEAGATVLDPDTMAPVPADGETLGEIMFRGNICMKG
YLKNEKATDEAFHGGWFHTGDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVAVAAVVAMPDPKWGEVP
CAFVELRDGASATEEEIVAHCRQLLAGFKVPKAVRFGELPKTSTGKIQKFQLRHAVGSAGAIDLAGDKK

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=545, Percent_Identity=29.5412844036697, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI187761345, Length=542, Percent_Identity=29.1512915129151, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI187761343, Length=542, Percent_Identity=29.1512915129151, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI58082049, Length=507, Percent_Identity=26.232741617357, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157311624, Length=509, Percent_Identity=25.5402750491159, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI157311622, Length=509, Percent_Identity=25.5402750491159, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI122937307, Length=511, Percent_Identity=26.4187866927593, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI38505220, Length=509, Percent_Identity=25.7367387033399, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI42544132, Length=511, Percent_Identity=25.8317025440313, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI115511026, Length=511, Percent_Identity=25.2446183953033, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI227499619, Length=558, Percent_Identity=24.0143369175627, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI57165410, Length=500, Percent_Identity=23.4, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI13375727, Length=158, Percent_Identity=36.0759493670886, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI13236579, Length=492, Percent_Identity=25, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI28416953, Length=144, Percent_Identity=31.25, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI8923896, Length=123, Percent_Identity=38.2113821138211, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI253970489, Length=123, Percent_Identity=38.2113821138211, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI221142682, Length=519, Percent_Identity=28.5163776493256, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788107, Length=545, Percent_Identity=26.4220183486239, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI145693145, Length=538, Percent_Identity=29.182156133829, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786810, Length=548, Percent_Identity=28.2846715328467, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786529, Length=537, Percent_Identity=25.1396648044693, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994703, Length=527, Percent_Identity=28.0834914611006, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71994694, Length=527, Percent_Identity=28.0834914611006, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71994690, Length=527, Percent_Identity=28.0834914611006, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17560308, Length=528, Percent_Identity=29.1666666666667, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17559526, Length=522, Percent_Identity=26.6283524904215, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17538037, Length=521, Percent_Identity=24.9520153550864, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=363, Percent_Identity=31.6804407713499, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=535, Percent_Identity=23.9252336448598, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=521, Percent_Identity=25.3358925143954, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=461, Percent_Identity=24.5119305856833, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=548, Percent_Identity=25.5474452554745, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=549, Percent_Identity=24.0437158469945, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17510401, Length=188, Percent_Identity=29.2553191489362, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71985884, Length=155, Percent_Identity=36.7741935483871, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=347, Percent_Identity=29.3948126801153, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=555, Percent_Identity=23.4234234234234, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=135, Percent_Identity=32.5925925925926, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581924, Length=556, Percent_Identity=28.0575539568345, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI21355181, Length=534, Percent_Identity=27.1535580524345, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI18859661, Length=555, Percent_Identity=26.6666666666667, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24583465, Length=547, Percent_Identity=24.6800731261426, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583463, Length=547, Percent_Identity=24.6800731261426, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364798, Length=547, Percent_Identity=24.6800731261426, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922652, Length=553, Percent_Identity=23.5081374321881, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356947, Length=519, Percent_Identity=22.1579961464355, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45550496, Length=555, Percent_Identity=24.8648648648649, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45552801, Length=546, Percent_Identity=24.9084249084249, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648253, Length=512, Percent_Identity=22.4609375, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI20130357, Length=356, Percent_Identity=22.752808988764, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648255, Length=507, Percent_Identity=22.0907297830375, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356441, Length=506, Percent_Identity=21.5415019762846, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648260, Length=511, Percent_Identity=23.091976516634, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=21.2424849699399, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281365686, Length=532, Percent_Identity=21.0526315789474, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59551; Mature: 59419

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMFEAGLGRREANYVPLTPIDFLVRSAEVYGDRVAIVHGDVRRTWAETYARARRLASA
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHH
LAAAGVGRGETVAALLPNIPAMVEAHFGVPMAGAVLNTINTRLDAASVLFMLRHGEAKVL
HHHHCCCCCCCHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
IVDTEYADIAQRAAQELPGLKLVSVADAMPADPARFAGAIDYEAFVASGDPDYAWHPPAD
EEECCHHHHHHHHHHHCCCEEEEEHHCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCC
EWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNILEWDMPKHAVYLWTLPMFHCNGWCF
CCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCH
PWAVAARAGVNVCLRKFDAKTVFDLIRRERVTHYCGAPIVQSAIANAPAELREGIDHTVH
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCHHHHHH
AMVAGAAPAPAVIAKMKEIGFDLLHVYGLTEVYGPATVCAKQGHWDALSDDERARLNARQ
HHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHC
GVRYHLEAGATVLDPDTMAPVPADGETLGEIMFRGNICMKGYLKNEKATDEAFHGGWFHT
CCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCCEEE
GDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVAVAAVVAMPDPKWGEV
CCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHHEEECCCCCCCCC
PCAFVELRDGASATEEEIVAHCRQLLAGFKVPKAVRFGELPKTSTGKIQKFQLRHAVGSA
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCC
GAIDLAGDKK
CEEECCCCCC
>Mature Secondary Structure 
TQMFEAGLGRREANYVPLTPIDFLVRSAEVYGDRVAIVHGDVRRTWAETYARARRLASA
CCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHH
LAAAGVGRGETVAALLPNIPAMVEAHFGVPMAGAVLNTINTRLDAASVLFMLRHGEAKVL
HHHHCCCCCCCHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
IVDTEYADIAQRAAQELPGLKLVSVADAMPADPARFAGAIDYEAFVASGDPDYAWHPPAD
EEECCHHHHHHHHHHHCCCEEEEEHHCCCCCCHHHHCCCCCCEEEEECCCCCCCCCCCCC
EWEAIALNYTSGTTGDPKGVVYHHRGAYLAAISNILEWDMPKHAVYLWTLPMFHCNGWCF
CCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCH
PWAVAARAGVNVCLRKFDAKTVFDLIRRERVTHYCGAPIVQSAIANAPAELREGIDHTVH
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCHHHHHH
AMVAGAAPAPAVIAKMKEIGFDLLHVYGLTEVYGPATVCAKQGHWDALSDDERARLNARQ
HHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHC
GVRYHLEAGATVLDPDTMAPVPADGETLGEIMFRGNICMKGYLKNEKATDEAFHGGWFHT
CCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCCEEE
GDLGVLTPDGYIRIKDRRKDIIISGGENISSIEVEDALYRHPAVAVAAVVAMPDPKWGEV
CCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCCHHHHHHEEECCCCCCCCC
PCAFVELRDGASATEEEIVAHCRQLLAGFKVPKAVRFGELPKTSTGKIQKFQLRHAVGSA
CEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCC
GAIDLAGDKK
CEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]