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The map label for this gene is ttgG [H]

Identifier: 189352916

GI number: 189352916

Start: 1065350

End: 1066510

Strand: Reverse

Name: ttgG [H]

Synonym: BMULJ_04150

Alternate gene names: 189352916

Gene position: 1066510-1065350 (Counterclockwise)

Preceding gene: 189352918

Following gene: 189352915

Centisome position: 43.12

GC content: 71.75

Gene sequence:

>1161_bases
ATGAAGAATCAGAAACGATGCTGCCACGGCGCCGTGTGCGCGCTGGTGGCGGCGATGCTGGCGGGTTGCGGGCCCGCCGA
ACGGCCGGTTGCCGCACCGCCCACGCCGGTCGCCGCGATGACGCTGACTGCCTCGCGCCTCGACGTGACCGAAGACTTGC
CGGGCCGCGTCGCGGCCGTGCGGATCGCCGAGATTCGGCCGCAGGTGAGCGGCATCGTACAGCGCCGGCTGTTCGAGCAA
GGCACCGAGGTCCGCACGGGCCAGCCGCTGTTCCAGATCAACCCCGCGCCGTTCAAGGCCGAAGTCGACACGGCCGCCGC
GGCGCTGCAGCGTGCGCAGGCGGCGCTCGAACGCGCGAAGGTGCAGACCGCGCGCTTCAAGCCGCTCGTCGAAGCCGATG
CGATCAGCCGCCAGGTGTACGACGACGCCGTGTCGCAACGCGACCAGGCCGCGGCCGACGTCGCGCAGGCGCGCGCGACG
CTCGCGCGCCGCCAGCTCGACCTGAAGTTCGCGACCGTCGAAGCGCCGATCGCGGGCCGCATCGATCAGGCGCTCGTGAC
CGAAGGCGCGCTGGTATCGAGCAGCGACAGCCAGCCGATGGCGCGCATCCAGCAGATCGACCAGGTCTACGTGGACGTGC
GCCAGCCGGCCGCATCGCTCGAAGCGCTGCGCGGCGCGCTCGCGGCGCAGCCGCAAGCGTCGGACGCCAATGGCGTGCCG
GTCGACGTGCTGCGCGCCGACGACTCGCGCTACGACGTGAAGGGCCGCATGCTGTTCTCGGGCGTCAACGTCGACCCCGG
CACCGGCGACGTGCTCGTGCGCGTGCTGGTGGACAACCCGAGGCGCGAGCTTCTGCCGGGCATGTACGTGCGTGCCCGCA
TCCCGCGCGCGCACTACGACCACGCGCTGACCGTGCCGCAGCAGGCAATCGTCCGCGCCGGCGGCAAGCCGCAGGTGTGG
GTGCTCGACGCCAAGGGCACGGCCCACCTGAAGGCCGTCGAAGTCGGCGAGCTGTCGAACCGCAACTACCGTATCAAGTC
GGGACTGCAGGCCGGACAGAAGGTCGTGGTCGAAGGCATGGAGCGCCTCGCCGAAGGCGCGTCCGTCGCGGCGAGCCCAT
GGCAGGCGCCCACGGCGGTCACGACCGCCTCCTCGCACTGA

Upstream 100 bases:

>100_bases
ATCGAATCTCCATCTTTCCGCATCAAAAAATCCATCGGGCGCACGCAGACTGCGAACCGAAAATGCGGGCCGTCGGGTGC
CTGCGTCCCATGGAGTCCAC

Downstream 100 bases:

>100_bases
GTCACGCGGAGCATCCAGTCATGTCTCAATTCTTCATCCGACGGCCGGTGTTCGCGTGGGTGATCGCCCTCTTCATCATC
CTGCTAGGCCTGATCGCGAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKNQKRCCHGAVCALVAAMLAGCGPAERPVAAPPTPVAAMTLTASRLDVTEDLPGRVAAVRIAEIRPQVSGIVQRRLFEQ
GTEVRTGQPLFQINPAPFKAEVDTAAAALQRAQAALERAKVQTARFKPLVEADAISRQVYDDAVSQRDQAAADVAQARAT
LARRQLDLKFATVEAPIAGRIDQALVTEGALVSSSDSQPMARIQQIDQVYVDVRQPAASLEALRGALAAQPQASDANGVP
VDVLRADDSRYDVKGRMLFSGVNVDPGTGDVLVRVLVDNPRRELLPGMYVRARIPRAHYDHALTVPQQAIVRAGGKPQVW
VLDAKGTAHLKAVEVGELSNRNYRIKSGLQAGQKVVVEGMERLAEGASVAASPWQAPTAVTTASSH

Sequences:

>Translated_386_residues
MKNQKRCCHGAVCALVAAMLAGCGPAERPVAAPPTPVAAMTLTASRLDVTEDLPGRVAAVRIAEIRPQVSGIVQRRLFEQ
GTEVRTGQPLFQINPAPFKAEVDTAAAALQRAQAALERAKVQTARFKPLVEADAISRQVYDDAVSQRDQAAADVAQARAT
LARRQLDLKFATVEAPIAGRIDQALVTEGALVSSSDSQPMARIQQIDQVYVDVRQPAASLEALRGALAAQPQASDANGVP
VDVLRADDSRYDVKGRMLFSGVNVDPGTGDVLVRVLVDNPRRELLPGMYVRARIPRAHYDHALTVPQQAIVRAGGKPQVW
VLDAKGTAHLKAVEVGELSNRNYRIKSGLQAGQKVVVEGMERLAEGASVAASPWQAPTAVTTASSH
>Mature_386_residues
MKNQKRCCHGAVCALVAAMLAGCGPAERPVAAPPTPVAAMTLTASRLDVTEDLPGRVAAVRIAEIRPQVSGIVQRRLFEQ
GTEVRTGQPLFQINPAPFKAEVDTAAAALQRAQAALERAKVQTARFKPLVEADAISRQVYDDAVSQRDQAAADVAQARAT
LARRQLDLKFATVEAPIAGRIDQALVTEGALVSSSDSQPMARIQQIDQVYVDVRQPAASLEALRGALAAQPQASDANGVP
VDVLRADDSRYDVKGRMLFSGVNVDPGTGDVLVRVLVDNPRRELLPGMYVRARIPRAHYDHALTVPQQAIVRAGGKPQVW
VLDAKGTAHLKAVEVGELSNRNYRIKSGLQAGQKVVVEGMERLAEGASVAASPWQAPTAVTTASSH

Specific function: The periplasmic linker component of an organic solvent efflux pump. Involved in export of a number of organic solvents, including toluene and styrene. This is the most important solvent efflux pump in this strain, although it can export AMP and some antib

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=366, Percent_Identity=35.792349726776, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1789929, Length=354, Percent_Identity=33.8983050847458, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1786668, Length=373, Percent_Identity=34.3163538873995, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87082036, Length=317, Percent_Identity=26.813880126183, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41138; Mature: 41138

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQKRCCHGAVCALVAAMLAGCGPAERPVAAPPTPVAAMTLTASRLDVTEDLPGRVAAV
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCEEEE
RIAEIRPQVSGIVQRRLFEQGTEVRTGQPLFQINPAPFKAEVDTAAAALQRAQAALERAK
EHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
VQTARFKPLVEADAISRQVYDDAVSQRDQAAADVAQARATLARRQLDLKFATVEAPIAGR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHCC
IDQALVTEGALVSSSDSQPMARIQQIDQVYVDVRQPAASLEALRGALAAQPQASDANGVP
HHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
VDVLRADDSRYDVKGRMLFSGVNVDPGTGDVLVRVLVDNPRRELLPGMYVRARIPRAHYD
EEEEECCCCCCCCCCEEEEECCCCCCCCHHEEEEEEECCCHHHHCCCCEEEEECCCHHCC
HALTVPQQAIVRAGGKPQVWVLDAKGTAHLKAVEVGELSNRNYRIKSGLQAGQKVVVEGM
CCCCCCHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHH
ERLAEGASVAASPWQAPTAVTTASSH
HHHHCCCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure
MKNQKRCCHGAVCALVAAMLAGCGPAERPVAAPPTPVAAMTLTASRLDVTEDLPGRVAAV
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCEEEE
RIAEIRPQVSGIVQRRLFEQGTEVRTGQPLFQINPAPFKAEVDTAAAALQRAQAALERAK
EHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
VQTARFKPLVEADAISRQVYDDAVSQRDQAAADVAQARATLARRQLDLKFATVEAPIAGR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHCC
IDQALVTEGALVSSSDSQPMARIQQIDQVYVDVRQPAASLEALRGALAAQPQASDANGVP
HHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
VDVLRADDSRYDVKGRMLFSGVNVDPGTGDVLVRVLVDNPRRELLPGMYVRARIPRAHYD
EEEEECCCCCCCCCCEEEEECCCCCCCCHHEEEEEEECCCHHHHCCCCEEEEECCCHHCC
HALTVPQQAIVRAGGKPQVWVLDAKGTAHLKAVEVGELSNRNYRIKSGLQAGQKVVVEGM
CCCCCCHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHH
ERLAEGASVAASPWQAPTAVTTASSH
HHHHCCCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11395460 [H]