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The map label for this gene is hpaG [H]

Identifier: 189352899

GI number: 189352899

Start: 1046333

End: 1047097

Strand: Direct

Name: hpaG [H]

Synonym: BMULJ_04133

Alternate gene names: 189352899

Gene position: 1046333-1047097 (Clockwise)

Preceding gene: 189352898

Following gene: 189352900

Centisome position: 42.31

GC content: 67.97

Gene sequence:

>765_bases
ATGAAGACGGCACGTGTCATCTACAACGGCGCCCTGCATGCGGCCGAACCGGCCGGCGACGGCGCGATCCGCCTCGATAC
CGGGCGCGTGCTCGGCGAGGAGGCCGTCGCGTGGCTGCCGCCCGTCGTGCCGCGCACGACCTTCGCGCTCGGCCTCAACT
ACGCGGATCACGCGAAGGAACTCGCGTTCAGCGCGCCGAGCGAGCCGCTGATCTTCCTGAAAGGGCCGAACACGTTCATC
GGCCACCGGTCGCAGACGGTGCGGCCTGCCGATGCGACGCACATGCACTACGAGTGCGAGCTCGCCGTCGTGATCGGCCG
GCCCGCGCGCAACGTCTCGCGCGCGCAGGCGATCGACTACGTGGCCGGCTATACGGTCGCGAACGACTACGCGATCCGCG
ACTATCTGGAGAACTACTATCGGCCGAATCTGCGCGTGAAGAATCGCGACACGTGCACGCCGCTCGGGCCGTGGTTCGTG
AGCCGCGACGAAGTCGGCGACGCCGGCGATCTCGCGCTGCGCACGACGGTCAACGGCCGCGAGACGCAACGCGGCAGCAC
GCGCGACATGATCTTCGACGTGCCCGCGCTGATCGAATACATCAGCGGCTTCATGACGCTCGCGCCGGGCGATCTGATTC
TGACCGGCACGCCGCAGGGGCTCGCCGACACGCAGCCCGGCGATGTGGTGGTGACGGAAATCGAACGAATCGGCCGGCTC
GTGAACACGATCGTCGGCGAAGCGGACTACTATCGCGCGGGCTGA

Upstream 100 bases:

>100_bases
TCGCGGGCGGCGCGCCGCATGCGCGCGTGGGCAGCACGATCGAGATCGCCGCCGACGGCATCGGCACGTTGCGCCACACG
CTCGTCGCGGAGGATGCACG

Downstream 100 bases:

>100_bases
GCCCGTCGCATAAGGAGAGCAGATGACCATCAAGCACTGGATCGACGGCCGCGAGGTCGACAGCCGCGACAGGTTCGCGA
CGCTGAATCCGGCGACGGGC

Product: 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKTARVIYNGALHAAEPAGDGAIRLDTGRVLGEEAVAWLPPVVPRTTFALGLNYADHAKELAFSAPSEPLIFLKGPNTFI
GHRSQTVRPADATHMHYECELAVVIGRPARNVSRAQAIDYVAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPLGPWFV
SRDEVGDAGDLALRTTVNGRETQRGSTRDMIFDVPALIEYISGFMTLAPGDLILTGTPQGLADTQPGDVVVTEIERIGRL
VNTIVGEADYYRAG

Sequences:

>Translated_254_residues
MKTARVIYNGALHAAEPAGDGAIRLDTGRVLGEEAVAWLPPVVPRTTFALGLNYADHAKELAFSAPSEPLIFLKGPNTFI
GHRSQTVRPADATHMHYECELAVVIGRPARNVSRAQAIDYVAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPLGPWFV
SRDEVGDAGDLALRTTVNGRETQRGSTRDMIFDVPALIEYISGFMTLAPGDLILTGTPQGLADTQPGDVVVTEIERIGRL
VNTIVGEADYYRAG
>Mature_254_residues
MKTARVIYNGALHAAEPAGDGAIRLDTGRVLGEEAVAWLPPVVPRTTFALGLNYADHAKELAFSAPSEPLIFLKGPNTFI
GHRSQTVRPADATHMHYECELAVVIGRPARNVSRAQAIDYVAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPLGPWFV
SRDEVGDAGDLALRTTVNGRETQRGSTRDMIFDVPALIEYISGFMTLAPGDLILTGTPQGLADTQPGDVVVTEIERIGRL
VNTIVGEADYYRAG

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=225, Percent_Identity=38.2222222222222, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI40786394, Length=225, Percent_Identity=38.2222222222222, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI215422413, Length=189, Percent_Identity=35.978835978836, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI13654274, Length=189, Percent_Identity=35.978835978836, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI66348062, Length=189, Percent_Identity=35.978835978836, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1787428, Length=183, Percent_Identity=36.6120218579235, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17557057, Length=185, Percent_Identity=38.9189189189189, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324161, Length=225, Percent_Identity=29.3333333333333, Blast_Score=96, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28572127, Length=219, Percent_Identity=42.0091324200913, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24663695, Length=216, Percent_Identity=36.5740740740741, Blast_Score=143, Evalue=9e-35,
Organism=Drosophila melanogaster, GI28571789, Length=206, Percent_Identity=38.3495145631068, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 27736; Mature: 27736

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTARVIYNGALHAAEPAGDGAIRLDTGRVLGEEAVAWLPPVVPRTTFALGLNYADHAKE
CCCCEEEECCCEECCCCCCCCEEEEECCCHHCHHHHHHCCCCCCCCEEEEECCHHHHHHH
LAFSAPSEPLIFLKGPNTFIGHRSQTVRPADATHMHYECELAVVIGRPARNVSRAQAIDY
HHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHH
VAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPLGPWFVSRDEVGDAGDLALRTTVNGR
HHCEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCC
ETQRGSTRDMIFDVPALIEYISGFMTLAPGDLILTGTPQGLADTQPGDVVVTEIERIGRL
CCCCCCCCCCEEEHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCEEHHHHHHHHHH
VNTIVGEADYYRAG
HHHHHCCCCCCCCC
>Mature Secondary Structure
MKTARVIYNGALHAAEPAGDGAIRLDTGRVLGEEAVAWLPPVVPRTTFALGLNYADHAKE
CCCCEEEECCCEECCCCCCCCEEEEECCCHHCHHHHHHCCCCCCCCEEEEECCHHHHHHH
LAFSAPSEPLIFLKGPNTFIGHRSQTVRPADATHMHYECELAVVIGRPARNVSRAQAIDY
HHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHH
VAGYTVANDYAIRDYLENYYRPNLRVKNRDTCTPLGPWFVSRDEVGDAGDLALRTTVNGR
HHCEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCC
ETQRGSTRDMIFDVPALIEYISGFMTLAPGDLILTGTPQGLADTQPGDVVVTEIERIGRL
CCCCCCCCCCEEEHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCEEHHHHHHHHHH
VNTIVGEADYYRAG
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA