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The map label for this gene is maiA [H]

Identifier: 189352835

GI number: 189352835

Start: 958086

End: 958727

Strand: Direct

Name: maiA [H]

Synonym: BMULJ_04069

Alternate gene names: 189352835

Gene position: 958086-958727 (Clockwise)

Preceding gene: 189352834

Following gene: 189352836

Centisome position: 38.74

GC content: 67.76

Gene sequence:

>642_bases
GTGCAACTGCATAGCTTCTTCAACAGTTCGACCTCCTATCGGGTGCGTATCGCGCTTGCGCTGAAAGGGCTGCCGTACGA
CACGCTGCCGGTGAACATCCGCATCGGTGCGCATCGCGACGCGCACTACGTCGACGACGTGAATCCTTCCGCGTCGGTGC
CCGCGCTCGTCGACGGCGATTTCCGCCTCGGTCAGTCGCTCGCGATCATCGACTATCTCGACGCGACGCATCCGCAGCCG
CGGCTGATTCCGGTCGATCCGCGACAGCGTGCGCGCGTGCTCGAGCTCGCGCTGCTGATCGCCTGCGACATCCATCCGGT
GAACAACCTGCGCGTGCTGCGCTATCTCGACGGCGAGCTCAACGTGACGCCGCAGCAGAAGACGGCGTGGTACCGGCACT
GGATCGCCGAAGGGATGGCGGGCGTCGAGCGCCTGCTCGCGCGCGCCGACACGGGGCCGTGGTGCTTCGGCGATGCACCG
ACGCTGGCCGACGTGTGTCTCGTCCCGCAGGTCGCGAACGCGCTGCGGATGGACTGCGATCTGCGCGCGTTTCCGCGCAG
CCTCGCGGTGTACGAACACGCGCAGCGCGAGCCGGCCTTCGTGGCCGCGCAGCCGCAGCGGCAACCGGATTACGTCGCCT
GA

Upstream 100 bases:

>100_bases
CGGCGCGGTCGTGCGCGGCGACGAGATGAAGGTCGGGGTCGAGCGGCTCGGCGAACTCACCGTGCGCGTCGTCTGACGCC
CGTTTTCGAAAGGTCCCATC

Downstream 100 bases:

>100_bases
GGCGATGGGGGAGACAGACATGAATCAGGCAGGAGACAAGGGCCGCCGCGTGCTCGTGATCGGCGGCGGCATCGGCGGGC
TCGCGACGGCGCTCGCGCTC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQLHSFFNSSTSYRVRIALALKGLPYDTLPVNIRIGAHRDAHYVDDVNPSASVPALVDGDFRLGQSLAIIDYLDATHPQP
RLIPVDPRQRARVLELALLIACDIHPVNNLRVLRYLDGELNVTPQQKTAWYRHWIAEGMAGVERLLARADTGPWCFGDAP
TLADVCLVPQVANALRMDCDLRAFPRSLAVYEHAQREPAFVAAQPQRQPDYVA

Sequences:

>Translated_213_residues
MQLHSFFNSSTSYRVRIALALKGLPYDTLPVNIRIGAHRDAHYVDDVNPSASVPALVDGDFRLGQSLAIIDYLDATHPQP
RLIPVDPRQRARVLELALLIACDIHPVNNLRVLRYLDGELNVTPQQKTAWYRHWIAEGMAGVERLLARADTGPWCFGDAP
TLADVCLVPQVANALRMDCDLRAFPRSLAVYEHAQREPAFVAAQPQRQPDYVA
>Mature_213_residues
MQLHSFFNSSTSYRVRIALALKGLPYDTLPVNIRIGAHRDAHYVDDVNPSASVPALVDGDFRLGQSLAIIDYLDATHPQP
RLIPVDPRQRARVLELALLIACDIHPVNNLRVLRYLDGELNVTPQQKTAWYRHWIAEGMAGVERLLARADTGPWCFGDAP
TLADVCLVPQVANALRMDCDLRAFPRSLAVYEHAQREPAFVAAQPQRQPDYVA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=209, Percent_Identity=42.1052631578947, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI22202622, Length=158, Percent_Identity=40.5063291139241, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=32.8571428571429, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=36.5384615384615, Blast_Score=129, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17510461, Length=208, Percent_Identity=31.7307692307692, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17556142, Length=198, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24645375, Length=209, Percent_Identity=41.6267942583732, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553325, Length=209, Percent_Identity=41.6267942583732, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21355859, Length=209, Percent_Identity=41.6267942583732, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI21355857, Length=210, Percent_Identity=38.5714285714286, Blast_Score=150, Evalue=7e-37,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23815; Mature: 23815

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLHSFFNSSTSYRVRIALALKGLPYDTLPVNIRIGAHRDAHYVDDVNPSASVPALVDGD
CCCHHHHCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEECCC
FRLGQSLAIIDYLDATHPQPRLIPVDPRQRARVLELALLIACDIHPVNNLRVLRYLDGEL
CCCCCEEEEEEHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCE
NVTPQQKTAWYRHWIAEGMAGVERLLARADTGPWCFGDAPTLADVCLVPQVANALRMDCD
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCC
LRAFPRSLAVYEHAQREPAFVAAQPQRQPDYVA
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MQLHSFFNSSTSYRVRIALALKGLPYDTLPVNIRIGAHRDAHYVDDVNPSASVPALVDGD
CCCHHHHCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEECCC
FRLGQSLAIIDYLDATHPQPRLIPVDPRQRARVLELALLIACDIHPVNNLRVLRYLDGEL
CCCCCEEEEEEHHCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCE
NVTPQQKTAWYRHWIAEGMAGVERLLARADTGPWCFGDAPTLADVCLVPQVANALRMDCD
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCC
LRAFPRSLAVYEHAQREPAFVAAQPQRQPDYVA
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]