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The map label for this gene is rpoS [H]

Identifier: 189352818

GI number: 189352818

Start: 938312

End: 939889

Strand: Reverse

Name: rpoS [H]

Synonym: BMULJ_04050

Alternate gene names: 189352818

Gene position: 939889-938312 (Counterclockwise)

Preceding gene: 189352823

Following gene: 189352817

Centisome position: 38.0

GC content: 72.37

Gene sequence:

>1578_bases
ATGACTACGCCACGCCCCACGTCCCGGGCGGACACGCCCCTCGATCCGATCATCCAGCTGCTCGCGCTGGCCAGCGAGCA
GGGTTATCTGACGCACGCCGACCTCGTCGACGCGCTGCCGCCCGAAAGCGACAGCCCCGATGCGCTCGATGTCGTGCGCG
CCGCGCTCGCGGACATCGGGATCACCGTGCTCGACGAGCCCGCCGCGCCGCCGCCGTTCGCGGCCGCGCCCGCGGTCGAC
GTCGATCGCGACACGCTCGAAGAGGGTCGCGCGCTGCTCGGCGATCTCGCGCGCGGCGCGAGCGCGTCGACCGATCCGCT
CGCGCTGTACATGCGCCGCATGCACGCGGTGCCGCTGCTCACGCGCGAAGACGAGATCGCGCTTGCGCGCGAGCTCGAAA
CCGGGCGCCACCAGATGCTGCATGCGCTCGCCGGTTGTCCGCCGGCGGTCGACGCGCTGCTGGCGCGCATCGGCACGACG
ACGGCGGCGAGCGCGTCCGACCGCAGCGACGGCGACGCGGACGATGCGGACGACACGAACGACAGCGCCGACGCGCCGGC
AGCCGCCCGCTTCGACGCGGTACGCGATGCACTCGCCGACGTGCGGCGCGCGCTACATGCGCACGGCTGCCGATCGAGCG
CCTATCGCGACGCCCGCGATCGTCTCGCGGCCGAGCTCGGCGCGCTGACCTGGATTGCCTCGGCCGTCGACGACGCAAGC
CGCGTCGTACACGCGCTTGCGGCCGCGTCGCGCGACGACGCGGCGCGTGCGATCGCCGGCGCGGACGCCGCGTGCTTCGA
CGCCGTTGCGCGCCGCGCACTGGTCGCGGCGCTGAACGACGGTCAACAGAAGGTGCGCGACGCGACGCGCGCGATGCTCG
AGGCGAACCTGCGGCTCGTGCTGTCGATCGCGCGCAAGTACCTGAACCGCGGCGTCGATCTGTCCGACCTCGTGCAGGAC
GGCTGTATCGGGTTGATGCGCGCGATCGAGAAGTTCGAATACCGGCGCGGATTCAAGTTTTCGACGTATGCGACGTGGTG
GATCCGGCAGGCGATCGCGCGGGCAGTCGCGGACCGCGCGCGGACGATCCGCGTGCCCGTCCACGTCGGCGATCAGCATC
AGCGCGTACGGCGGCAGGCACTGCGCTTCCGTCAGCGCACGGGCCGTCAGCCGACGCCGGCCGAACTGGCCGCCGAAACC
GGCCTGGCGGAGGACAAGCTGCGTGCGATTCTCGCGCTGCCGGCCGAGCCGGTCTCGCTCGACACGCCGCTGCCGGACGC
CGACACCGGGCTCGTCGACCTGATCGAGGACGAAACCTCCGCGAGCCCGTTCGAGCAGCTGGCCGGCACGCGCATGCGCG
AATGCGTGCGCTCGCTGCTGCGTTCGGTCACGCCGACCGAGGCCGACGTGCTGCGCCGGCGCTTCGGTCTCGGCGGCACC
GAGCCGGACACCTACGACGCGATCGCGAAGGATGCCGGGATGTCGCGCGAGCGCGTGCGCCAGATCGAGAAGCGTGCACT
CGCCGCGCTGCGCACGGCCGCCGAAGCCGAAGGCGCACACGCGTTTCTGGACGCATAA

Upstream 100 bases:

>100_bases
CCGGCCGGCGTGAACGAACAGGCGTTCGCGAACACCCGGCGGCCCCAAACCGGCGGGCTTCGGTCCGCCTCACAGGAACG
CGGCCCGCGCCGCCATCGAC

Downstream 100 bases:

>100_bases
TCGTCGTACGCGGCGCGCGTCCGGCAACGTGCAACGTTTGCCATTGATGCACGTCAATCGCCGTTCGACACGCATCGCGA
CAATCGTCGAAGGACCGGGC

Product: RNA polymerase sigma 38 subunit

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTTPRPTSRADTPLDPIIQLLALASEQGYLTHADLVDALPPESDSPDALDVVRAALADIGITVLDEPAAPPPFAAAPAVD
VDRDTLEEGRALLGDLARGASASTDPLALYMRRMHAVPLLTREDEIALARELETGRHQMLHALAGCPPAVDALLARIGTT
TAASASDRSDGDADDADDTNDSADAPAAARFDAVRDALADVRRALHAHGCRSSAYRDARDRLAAELGALTWIASAVDDAS
RVVHALAAASRDDAARAIAGADAACFDAVARRALVAALNDGQQKVRDATRAMLEANLRLVLSIARKYLNRGVDLSDLVQD
GCIGLMRAIEKFEYRRGFKFSTYATWWIRQAIARAVADRARTIRVPVHVGDQHQRVRRQALRFRQRTGRQPTPAELAAET
GLAEDKLRAILALPAEPVSLDTPLPDADTGLVDLIEDETSASPFEQLAGTRMRECVRSLLRSVTPTEADVLRRRFGLGGT
EPDTYDAIAKDAGMSRERVRQIEKRALAALRTAAEAEGAHAFLDA

Sequences:

>Translated_525_residues
MTTPRPTSRADTPLDPIIQLLALASEQGYLTHADLVDALPPESDSPDALDVVRAALADIGITVLDEPAAPPPFAAAPAVD
VDRDTLEEGRALLGDLARGASASTDPLALYMRRMHAVPLLTREDEIALARELETGRHQMLHALAGCPPAVDALLARIGTT
TAASASDRSDGDADDADDTNDSADAPAAARFDAVRDALADVRRALHAHGCRSSAYRDARDRLAAELGALTWIASAVDDAS
RVVHALAAASRDDAARAIAGADAACFDAVARRALVAALNDGQQKVRDATRAMLEANLRLVLSIARKYLNRGVDLSDLVQD
GCIGLMRAIEKFEYRRGFKFSTYATWWIRQAIARAVADRARTIRVPVHVGDQHQRVRRQALRFRQRTGRQPTPAELAAET
GLAEDKLRAILALPAEPVSLDTPLPDADTGLVDLIEDETSASPFEQLAGTRMRECVRSLLRSVTPTEADVLRRRFGLGGT
EPDTYDAIAKDAGMSRERVRQIEKRALAALRTAAEAEGAHAFLDA
>Mature_524_residues
TTPRPTSRADTPLDPIIQLLALASEQGYLTHADLVDALPPESDSPDALDVVRAALADIGITVLDEPAAPPPFAAAPAVDV
DRDTLEEGRALLGDLARGASASTDPLALYMRRMHAVPLLTREDEIALARELETGRHQMLHALAGCPPAVDALLARIGTTT
AASASDRSDGDADDADDTNDSADAPAAARFDAVRDALADVRRALHAHGCRSSAYRDARDRLAAELGALTWIASAVDDASR
VVHALAAASRDDAARAIAGADAACFDAVARRALVAALNDGQQKVRDATRAMLEANLRLVLSIARKYLNRGVDLSDLVQDG
CIGLMRAIEKFEYRRGFKFSTYATWWIRQAIARAVADRARTIRVPVHVGDQHQRVRRQALRFRQRTGRQPTPAELAAETG
LAEDKLRAILALPAEPVSLDTPLPDADTGLVDLIEDETSASPFEQLAGTRMRECVRSLLRSVTPTEADVLRRRFGLGGTE
PDTYDAIAKDAGMSRERVRQIEKRALAALRTAAEAEGAHAFLDA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=48.1632653061224, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1789098, Length=232, Percent_Identity=39.6551724137931, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789871, Length=249, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 56402; Mature: 56271

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00715 SIGMA70_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPRPTSRADTPLDPIIQLLALASEQGYLTHADLVDALPPESDSPDALDVVRAALADIG
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
ITVLDEPAAPPPFAAAPAVDVDRDTLEEGRALLGDLARGASASTDPLALYMRRMHAVPLL
CEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
TREDEIALARELETGRHQMLHALAGCPPAVDALLARIGTTTAASASDRSDGDADDADDTN
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
DSADAPAAARFDAVRDALADVRRALHAHGCRSSAYRDARDRLAAELGALTWIASAVDDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVVHALAAASRDDAARAIAGADAACFDAVARRALVAALNDGQQKVRDATRAMLEANLRLV
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LSIARKYLNRGVDLSDLVQDGCIGLMRAIEKFEYRRGFKFSTYATWWIRQAIARAVADRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RTIRVPVHVGDQHQRVRRQALRFRQRTGRQPTPAELAAETGLAEDKLRAILALPAEPVSL
HEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCC
DTPLPDADTGLVDLIEDETSASPFEQLAGTRMRECVRSLLRSVTPTEADVLRRRFGLGGT
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
EPDTYDAIAKDAGMSRERVRQIEKRALAALRTAAEAEGAHAFLDA
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
TTPRPTSRADTPLDPIIQLLALASEQGYLTHADLVDALPPESDSPDALDVVRAALADIG
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
ITVLDEPAAPPPFAAAPAVDVDRDTLEEGRALLGDLARGASASTDPLALYMRRMHAVPLL
CEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
TREDEIALARELETGRHQMLHALAGCPPAVDALLARIGTTTAASASDRSDGDADDADDTN
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
DSADAPAAARFDAVRDALADVRRALHAHGCRSSAYRDARDRLAAELGALTWIASAVDDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVVHALAAASRDDAARAIAGADAACFDAVARRALVAALNDGQQKVRDATRAMLEANLRLV
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LSIARKYLNRGVDLSDLVQDGCIGLMRAIEKFEYRRGFKFSTYATWWIRQAIARAVADRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RTIRVPVHVGDQHQRVRRQALRFRQRTGRQPTPAELAAETGLAEDKLRAILALPAEPVSL
HEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCC
DTPLPDADTGLVDLIEDETSASPFEQLAGTRMRECVRSLLRSVTPTEADVLRRRFGLGGT
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
EPDTYDAIAKDAGMSRERVRQIEKRALAALRTAAEAEGAHAFLDA
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]