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The map label for this gene is emrB [H]

Identifier: 189352813

GI number: 189352813

Start: 934038

End: 935564

Strand: Reverse

Name: emrB [H]

Synonym: BMULJ_04045

Alternate gene names: 189352813

Gene position: 935564-934038 (Counterclockwise)

Preceding gene: 189352815

Following gene: 189352811

Centisome position: 37.83

GC content: 66.86

Gene sequence:

>1527_bases
TTGAACCCCGCCGCAACCGCGTCGCCGCGCCCGCTGAACCGGCCGATGCTGACCCTGTCGATCATGCTCGCCACGCTGAT
CCAGACGCTCGACAGCACGATCGCCAACGTCGCGCTGCCGCACATGCAAGGCAGCCTCGCCGCCTCGCAGGACGAGATCA
CGTGGGTGCTGACCTCCTATATCGTCGCCGCGGCGATCGCGACGCCGCTGACCGGCTGGCTATCCGACCGCCTCAGCGTG
AAGCGGCTGCTGACGATCTCGATCGCGGGCTTCACGGTCGCGTCGGCGCTCTGCGGGCTATCGGAGTCGCTCGTGCAGAT
CGTCGGCTCGCGGCTGCTGCAGGGCGTGTTCGGCGCGGCACTGGTGCCGCTCTCGCAATCGATCCTGCTCGACATCAATC
CGCGCGAGAAACAGGGCCAGGCGATGGCGATCTGGGGCATGGGCGTGATGGTCGGTCCGATCCTCGGCCCGACGCTCGGC
GGCTGGCTTACCGACAGCTACAACTGGCGCTGGGTATTCTTCATCAACGTCCCGATCGGCGCGTTCGCGCTGGCCGGTGT
GCTGACCTTCTTGCCCGCACGCGCGCCGCGTCCGCACGTGAAATTCGACGCATTCGGCTTCGCGACGCTCGCGCTGGCGA
TCGGCGCGTTCCAGGCGATGCTCGACCGCGGCGAGCAGCTCGACTGGTTCGGCTCGACCGAGATCCGCATCGAGGCGCTG
GCCGCCGCGCTCAGCTTCGCGTTCTTCCTCGCGCATACGGCGACCGTCGGCAAGGCCTCGTTCTTCAAGTCGGAACTGCT
GCGCGACCCGAACTTCGCGACCGGCACGCTGTTCATCTTCGTCGTCGGCGCGGTTTTGTACGCGACGCGCGCGCTGCTGC
CGCCGATGCTGCAGAACCTGATGGGCTATCCGGTCGCGACGACGGGCCTCGTCACGGCGCCGAGCGGCGCCGGCACGATG
ATCGCGATGCTGTTCGTCGGGCGGCTGCTGAAATACGTCGATGCGCGGCTGCTATTGCTCGCGGGGTTGTCGATCTCCGC
GCTCGCGCTGTGGCAGATGATGCGCTATACGATCGTGCTGTCCGAGGCCGATATCGTGTGGCCGGGCGTGATCCAGGGTT
TCGGGCTCGGGCTCGTGTTCGTGCCGCTGAGCGCGATGTCGTTCTCGACGCTCGCGCCCGAGCTGCGTGCGGACGGCACC
GCGACTTACAGCCTGATGCGCAACATCGGCAGCAGCATCGGCATTTCGATCGTGCAGACGCTGATGACGCGCAACACGCA
GGTCGCGCACGCCGATCTCGCCACGCAGATCACGCCGTTCAACCCTGCCGTGCAAGCCATGGCCGGCAGCCACCTCGACA
TGGCGCTGCTCGACCGCACGATCAACCAGCAGGCCGCGATGATCGCGTATCTCAACGATTTCAAGCTGATGTTCGTCGCG
ACGCTGCTGATGATTCCGTTGCTGCTGCTGATCCGCCCGCCGCGCAAGGGCGCGGACGTCGACGTCGCGCACGCGGCGAT
GGACTAA

Upstream 100 bases:

>100_bases
CGCGCGCCGCAGCGACGATAAGGGGATATACAATAAGCGTGCTTATAATTTGACGCATACCGATCGCCGCCCACGGCGGC
CGCCCCACGGAGACACTTCC

Downstream 100 bases:

>100_bases
CGCCGCGTGCGCGCCGGTGCGGCGCGATCAGGCCTTCCGGCCGACCTCCGGTCAGACCTTCATCGTGCCGGTACGCACGA
AGCGCTCGTGCCACGACAGC

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNPAATASPRPLNRPMLTLSIMLATLIQTLDSTIANVALPHMQGSLAASQDEITWVLTSYIVAAAIATPLTGWLSDRLSV
KRLLTISIAGFTVASALCGLSESLVQIVGSRLLQGVFGAALVPLSQSILLDINPREKQGQAMAIWGMGVMVGPILGPTLG
GWLTDSYNWRWVFFINVPIGAFALAGVLTFLPARAPRPHVKFDAFGFATLALAIGAFQAMLDRGEQLDWFGSTEIRIEAL
AAALSFAFFLAHTATVGKASFFKSELLRDPNFATGTLFIFVVGAVLYATRALLPPMLQNLMGYPVATTGLVTAPSGAGTM
IAMLFVGRLLKYVDARLLLLAGLSISALALWQMMRYTIVLSEADIVWPGVIQGFGLGLVFVPLSAMSFSTLAPELRADGT
ATYSLMRNIGSSIGISIVQTLMTRNTQVAHADLATQITPFNPAVQAMAGSHLDMALLDRTINQQAAMIAYLNDFKLMFVA
TLLMIPLLLLIRPPRKGADVDVAHAAMD

Sequences:

>Translated_508_residues
MNPAATASPRPLNRPMLTLSIMLATLIQTLDSTIANVALPHMQGSLAASQDEITWVLTSYIVAAAIATPLTGWLSDRLSV
KRLLTISIAGFTVASALCGLSESLVQIVGSRLLQGVFGAALVPLSQSILLDINPREKQGQAMAIWGMGVMVGPILGPTLG
GWLTDSYNWRWVFFINVPIGAFALAGVLTFLPARAPRPHVKFDAFGFATLALAIGAFQAMLDRGEQLDWFGSTEIRIEAL
AAALSFAFFLAHTATVGKASFFKSELLRDPNFATGTLFIFVVGAVLYATRALLPPMLQNLMGYPVATTGLVTAPSGAGTM
IAMLFVGRLLKYVDARLLLLAGLSISALALWQMMRYTIVLSEADIVWPGVIQGFGLGLVFVPLSAMSFSTLAPELRADGT
ATYSLMRNIGSSIGISIVQTLMTRNTQVAHADLATQITPFNPAVQAMAGSHLDMALLDRTINQQAAMIAYLNDFKLMFVA
TLLMIPLLLLIRPPRKGADVDVAHAAMD
>Mature_508_residues
MNPAATASPRPLNRPMLTLSIMLATLIQTLDSTIANVALPHMQGSLAASQDEITWVLTSYIVAAAIATPLTGWLSDRLSV
KRLLTISIAGFTVASALCGLSESLVQIVGSRLLQGVFGAALVPLSQSILLDINPREKQGQAMAIWGMGVMVGPILGPTLG
GWLTDSYNWRWVFFINVPIGAFALAGVLTFLPARAPRPHVKFDAFGFATLALAIGAFQAMLDRGEQLDWFGSTEIRIEAL
AAALSFAFFLAHTATVGKASFFKSELLRDPNFATGTLFIFVVGAVLYATRALLPPMLQNLMGYPVATTGLVTAPSGAGTM
IAMLFVGRLLKYVDARLLLLAGLSISALALWQMMRYTIVLSEADIVWPGVIQGFGLGLVFVPLSAMSFSTLAPELRADGT
ATYSLMRNIGSSIGISIVQTLMTRNTQVAHADLATQITPFNPAVQAMAGSHLDMALLDRTINQQAAMIAYLNDFKLMFVA
TLLMIPLLLLIRPPRKGADVDVAHAAMD

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=438, Percent_Identity=38.8127853881278, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1788710, Length=512, Percent_Identity=33.984375, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790195, Length=417, Percent_Identity=29.2565947242206, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=28.7037037037037, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788392, Length=424, Percent_Identity=29.4811320754717, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI87082320, Length=172, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=150, Percent_Identity=30, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=136, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=388, Percent_Identity=25.2577319587629, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=185, Percent_Identity=25.9459459459459, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54282; Mature: 54282

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAATASPRPLNRPMLTLSIMLATLIQTLDSTIANVALPHMQGSLAASQDEITWVLTSY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
IVAAAIATPLTGWLSDRLSVKRLLTISIAGFTVASALCGLSESLVQIVGSRLLQGVFGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LVPLSQSILLDINPREKQGQAMAIWGMGVMVGPILGPTLGGWLTDSYNWRWVFFINVPIG
HHCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCHH
AFALAGVLTFLPARAPRPHVKFDAFGFATLALAIGAFQAMLDRGEQLDWFGSTEIRIEAL
HHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AAALSFAFFLAHTATVGKASFFKSELLRDPNFATGTLFIFVVGAVLYATRALLPPMLQNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGYPVATTGLVTAPSGAGTMIAMLFVGRLLKYVDARLLLLAGLSISALALWQMMRYTIVL
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEE
SEADIVWPGVIQGFGLGLVFVPLSAMSFSTLAPELRADGTATYSLMRNIGSSIGISIVQT
ECCCEECCHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCCHHHHHHHHH
LMTRNTQVAHADLATQITPFNPAVQAMAGSHLDMALLDRTINQQAAMIAYLNDFKLMFVA
HHHCCCCEEHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLLMIPLLLLIRPPRKGADVDVAHAAMD
HHHHHHHHHHCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MNPAATASPRPLNRPMLTLSIMLATLIQTLDSTIANVALPHMQGSLAASQDEITWVLTSY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
IVAAAIATPLTGWLSDRLSVKRLLTISIAGFTVASALCGLSESLVQIVGSRLLQGVFGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LVPLSQSILLDINPREKQGQAMAIWGMGVMVGPILGPTLGGWLTDSYNWRWVFFINVPIG
HHCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCHH
AFALAGVLTFLPARAPRPHVKFDAFGFATLALAIGAFQAMLDRGEQLDWFGSTEIRIEAL
HHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AAALSFAFFLAHTATVGKASFFKSELLRDPNFATGTLFIFVVGAVLYATRALLPPMLQNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGYPVATTGLVTAPSGAGTMIAMLFVGRLLKYVDARLLLLAGLSISALALWQMMRYTIVL
CCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEE
SEADIVWPGVIQGFGLGLVFVPLSAMSFSTLAPELRADGTATYSLMRNIGSSIGISIVQT
ECCCEECCHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCCHHHHHHHHH
LMTRNTQVAHADLATQITPFNPAVQAMAGSHLDMALLDRTINQQAAMIAYLNDFKLMFVA
HHHCCCCEEHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLLMIPLLLLIRPPRKGADVDVAHAAMD
HHHHHHHHHHCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]