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The map label for this gene is gcvA [H]

Identifier: 189352785

GI number: 189352785

Start: 898447

End: 899349

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_04017

Alternate gene names: 189352785

Gene position: 899349-898447 (Counterclockwise)

Preceding gene: 189352789

Following gene: 189352784

Centisome position: 36.36

GC content: 68.44

Gene sequence:

>903_bases
ATGAAAAATATCGAACAGTTGCACGACGATCCACCGTTGCGCGCCGTGCGCGCGTTCGAAGCGTTCGCGCGCCTCGGCTC
GGTCACGGCGGCGGCGGCCGAACTCGACATCACGCCGTCCGCGGTCAGCCATCAGCTGCAACTGCTGGAGGCGTTCATCC
AGACGCCGCTGACCGTACGCGACGGCCGGCTGCTCGCGCTGACCGACGAAGGACGCGACTACTACCGGTCGATCAGCGCG
GCGTTCTCGGTACTGCGCAGCGCGACGCGCTTCGTGCGCGACCGTTCGTCGCTGCGGCAGATCACGATCAGCCTGATACC
GCTGTTCGGCATCGGCTGGTTCATTCCGCGGCTGCACGCGTTTCTCGCGGAGAACACGGACGTCGACGTCACCGTCCTCT
ACGCGCACCACCGCAACTACCGCAGCGATGCGTCCGATCTGTCGATCCGCTTCGGCACGGGCGACTGGCCCGGCTATCGC
TGCGAACGGCTGGTGCCGGGCGCGATGGTGCCGATGTGCAGCCCGTCGTTCCTGAAGCGCCACGGGCCGTTTGCCACGCC
GGCCGATCTCGCGCATGCGCCGCTCGTGCACGACGAGGATCGCAGCGCGTGGGTGAACTGGCTGCAAGGCGCGGGCGTGC
GCAACGTGTCGCACGCGGTCGGCCCGATGTTCGAGGACGGGCAGCTCACGTTGAGCGCAGCACGCGCGGATCTCGGCGTC
GCGCTGCTGCGCGCGCCGCTCGTCGAGCGCGAACTCGCGAGCGGCGAACTCGTGCAGCTGTTCGATCACGCGCTCGAGGA
CGGCCGCGACTACTACCTGTGCACGCGCGAGGACGCGGACTTGCCGGACGGCGCGAGGCGGCTCGCGGAATGGTTGAGGG
AGATGGCTGGAGTGCACGGTTGA

Upstream 100 bases:

>100_bases
TGGATGCTTATGCCGTTCCGCACACGATACGCCAACACCATGAAAACATGGTTATTTTTCTTGTTGTCGCTTACCCTGAT
TTTTCTATAAAATTCGATAC

Downstream 100 bases:

>100_bases
CATAGCCGTTCGCATTGCCGCAGCATGCCCGTTCACAGCAACGGGCAGCACGCCCTTTGGCGCTCTACGAGCGTCCTACA
GTCGGAGGGTGACAATGCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKNIEQLHDDPPLRAVRAFEAFARLGSVTAAAAELDITPSAVSHQLQLLEAFIQTPLTVRDGRLLALTDEGRDYYRSISA
AFSVLRSATRFVRDRSSLRQITISLIPLFGIGWFIPRLHAFLAENTDVDVTVLYAHHRNYRSDASDLSIRFGTGDWPGYR
CERLVPGAMVPMCSPSFLKRHGPFATPADLAHAPLVHDEDRSAWVNWLQGAGVRNVSHAVGPMFEDGQLTLSAARADLGV
ALLRAPLVERELASGELVQLFDHALEDGRDYYLCTREDADLPDGARRLAEWLREMAGVHG

Sequences:

>Translated_300_residues
MKNIEQLHDDPPLRAVRAFEAFARLGSVTAAAAELDITPSAVSHQLQLLEAFIQTPLTVRDGRLLALTDEGRDYYRSISA
AFSVLRSATRFVRDRSSLRQITISLIPLFGIGWFIPRLHAFLAENTDVDVTVLYAHHRNYRSDASDLSIRFGTGDWPGYR
CERLVPGAMVPMCSPSFLKRHGPFATPADLAHAPLVHDEDRSAWVNWLQGAGVRNVSHAVGPMFEDGQLTLSAARADLGV
ALLRAPLVERELASGELVQLFDHALEDGRDYYLCTREDADLPDGARRLAEWLREMAGVHG
>Mature_300_residues
MKNIEQLHDDPPLRAVRAFEAFARLGSVTAAAAELDITPSAVSHQLQLLEAFIQTPLTVRDGRLLALTDEGRDYYRSISA
AFSVLRSATRFVRDRSSLRQITISLIPLFGIGWFIPRLHAFLAENTDVDVTVLYAHHRNYRSDASDLSIRFGTGDWPGYR
CERLVPGAMVPMCSPSFLKRHGPFATPADLAHAPLVHDEDRSAWVNWLQGAGVRNVSHAVGPMFEDGQLTLSAARADLGV
ALLRAPLVERELASGELVQLFDHALEDGRDYYLCTREDADLPDGARRLAEWLREMAGVHG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=273, Percent_Identity=38.0952380952381, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=31.0580204778157, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=32.1285140562249, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI87081978, Length=274, Percent_Identity=25.1824817518248, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=26.158940397351, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33281; Mature: 33281

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIEQLHDDPPLRAVRAFEAFARLGSVTAAAAELDITPSAVSHQLQLLEAFIQTPLTVR
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
DGRLLALTDEGRDYYRSISAAFSVLRSATRFVRDRSSLRQITISLIPLFGIGWFIPRLHA
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAENTDVDVTVLYAHHRNYRSDASDLSIRFGTGDWPGYRCERLVPGAMVPMCSPSFLKR
HHHCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCHHHHHH
HGPFATPADLAHAPLVHDEDRSAWVNWLQGAGVRNVSHAVGPMFEDGQLTLSAARADLGV
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEHHHHHHHH
ALLRAPLVERELASGELVQLFDHALEDGRDYYLCTREDADLPDGARRLAEWLREMAGVHG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNIEQLHDDPPLRAVRAFEAFARLGSVTAAAAELDITPSAVSHQLQLLEAFIQTPLTVR
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
DGRLLALTDEGRDYYRSISAAFSVLRSATRFVRDRSSLRQITISLIPLFGIGWFIPRLHA
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLAENTDVDVTVLYAHHRNYRSDASDLSIRFGTGDWPGYRCERLVPGAMVPMCSPSFLKR
HHHCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCHHHHHH
HGPFATPADLAHAPLVHDEDRSAWVNWLQGAGVRNVSHAVGPMFEDGQLTLSAARADLGV
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEHHHHHHHH
ALLRAPLVERELASGELVQLFDHALEDGRDYYLCTREDADLPDGARRLAEWLREMAGVHG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]