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The map label for this gene is ahpF [H]

Identifier: 189352782

GI number: 189352782

Start: 895654

End: 897252

Strand: Reverse

Name: ahpF [H]

Synonym: BMULJ_04014

Alternate gene names: 189352782

Gene position: 897252-895654 (Counterclockwise)

Preceding gene: 189352783

Following gene: 189352776

Centisome position: 36.28

GC content: 66.54

Gene sequence:

>1599_bases
ATGCTCGACGCCAATCTCAAGACCCAACTCAAAGCGTACCTCGAAAAAATCACGCGCCCGATCGAACTCGTCGCATCGCT
CGACGACAGCGCGAAGTCGCAGGAACTGCGTGCGCTGCTCGACGAGATTGCATCGCTGACGACGCGCGTCACCGTGATCG
AACGCCGCGACGACGAGGCGCGCACGCCGTCGTTCTCGATCGGCGAACCGGGCCAGCCCGCGCGGATCCGCTTCGCGGGC
ATTCCGATGGGCCATGAATTCACGTCGCTCGTGCTCGCGCTGCTGCAGACGGGCGGCCATCCGGTCAAGCTCGACGACGA
CGTGATCGAACAGATCCGCGCGCTCGACGGCGACTATGCGTTCGAGACGTACTTCTCGCTGTCGTGCCAGAACTGCCCGG
AAGTCGTGCAGGCGCTGAACGTGATGTCGCTGATCAACCCGCGCATCCGTCACGTCGCGATCGACGGCGCGCTGTTCCAG
GACGAAGTCGAAGCACGCCAGATCATGGCGGTGCCGACGATGTTCCTGAACGGCGAATCGTTCGGCCAGGGCCGCAGCAG
CGTGAAGGAAATCCTCGCGAAGCTCGATACGGGCGCCGGCGCACGCGCGGCGAAGTCGCTCGAGAACAAGCCGGTGTTCG
ACACGCTGATCGTCGGCGGCGGCCCGGCCGGTGCGGCGGCCGCGATCTACTCGGCGCGCAAGGGCATCGCGACCGGCGTG
GTCGCCGAACGTTTCGGCGGCCAGGTGCTCGACACGATGGCGATCGAGAACTTCGTGTCGGTGCAGGAAACCGAAGGACC
GAAGTTCGCGACTGCGCTCGAACAACACGTGAAGCAGTACGACGTCGACATCATGGACGTGCAGCGCGCCGAAGCGCTGA
TTCCCGGCGACGTGCATCAGATCCGCCTCGCGAACGGCGCCGTGCTGAAGGCCAGGACGGTCGTGCTCGCGACGGGCGCG
CGCTGGCGCGAGATCAACGTGCCCGGCGAACGCGAATACCGCAACCGCGGCGTCGCGTACTGCCCGCACTGCGACGGCCC
GCTGTTCAAGGGCAAGCGCGTCGCGGTCGTCGGCGGCGGCAACTCGGGCGTCGAGGCCGCGATCGATCTCGCGGGCATCG
TGAAGGCAGTCACGCTGATCGAATACGGCGCGCAGCTGCGCGCGGACGAAGTGCTGCAACGCAAGCTGCGCAGCCTGCCG
AACGTGACCGTCATCACCGAAGCGCAGACGACCGAGCTGACCGGCGACGGCAGCAAGCTGAACGGGCTCGTGTATACGGA
CCGTCGCTCGGGCGAGGTGAAGCGGATCGAACTCGAAGGCGTGTTCGTGCAGATCGGCCTCGTGCCGAACACGGAATGGC
TGAAGGGCACGGTCGAGCTGTCGAAGCACGGCGAGATCGTCGTCGATGCACGCGGCGCGACGTCGGTGCCCGGCGTGTTC
GCGGCCGGCGACGTGACGACGGTGCCGTTCAAGCAGATCGTGATTGCGGTCGGCGAAGGCGCCAAGGCGTCGCTCGGTGC
ATTCGATCATCTGATCCGCCAGGACGCGACGCCGGCTGTCGACACGCAACCGCAAGCCGACGAAGCGGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CACAGGCGCGCGACGGCGGGCCGCCGCATCGCGCGGCCCGCATCCCCTTCTCCGGCATGCAGTGCCCGGCCGGAACCTTC
GCTACGGAATCCGAAACGCC

Downstream 100 bases:

>100_bases
CACTCGACACAACGCATCTCTCTTCGCCCCGCATGCGCTGGTCAGCGTGCGGGGCGCTTTTTTATTTCGCGGGGGCGTTG
TCGCGCGGCGGCGGCGCGCT

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MLDANLKTQLKAYLEKITRPIELVASLDDSAKSQELRALLDEIASLTTRVTVIERRDDEARTPSFSIGEPGQPARIRFAG
IPMGHEFTSLVLALLQTGGHPVKLDDDVIEQIRALDGDYAFETYFSLSCQNCPEVVQALNVMSLINPRIRHVAIDGALFQ
DEVEARQIMAVPTMFLNGESFGQGRSSVKEILAKLDTGAGARAAKSLENKPVFDTLIVGGGPAGAAAAIYSARKGIATGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDIMDVQRAEALIPGDVHQIRLANGAVLKARTVVLATGA
RWREINVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVKAVTLIEYGAQLRADEVLQRKLRSLP
NVTVITEAQTTELTGDGSKLNGLVYTDRRSGEVKRIELEGVFVQIGLVPNTEWLKGTVELSKHGEIVVDARGATSVPGVF
AAGDVTTVPFKQIVIAVGEGAKASLGAFDHLIRQDATPAVDTQPQADEAVAA

Sequences:

>Translated_532_residues
MLDANLKTQLKAYLEKITRPIELVASLDDSAKSQELRALLDEIASLTTRVTVIERRDDEARTPSFSIGEPGQPARIRFAG
IPMGHEFTSLVLALLQTGGHPVKLDDDVIEQIRALDGDYAFETYFSLSCQNCPEVVQALNVMSLINPRIRHVAIDGALFQ
DEVEARQIMAVPTMFLNGESFGQGRSSVKEILAKLDTGAGARAAKSLENKPVFDTLIVGGGPAGAAAAIYSARKGIATGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDIMDVQRAEALIPGDVHQIRLANGAVLKARTVVLATGA
RWREINVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVKAVTLIEYGAQLRADEVLQRKLRSLP
NVTVITEAQTTELTGDGSKLNGLVYTDRRSGEVKRIELEGVFVQIGLVPNTEWLKGTVELSKHGEIVVDARGATSVPGVF
AAGDVTTVPFKQIVIAVGEGAKASLGAFDHLIRQDATPAVDTQPQADEAVAA
>Mature_532_residues
MLDANLKTQLKAYLEKITRPIELVASLDDSAKSQELRALLDEIASLTTRVTVIERRDDEARTPSFSIGEPGQPARIRFAG
IPMGHEFTSLVLALLQTGGHPVKLDDDVIEQIRALDGDYAFETYFSLSCQNCPEVVQALNVMSLINPRIRHVAIDGALFQ
DEVEARQIMAVPTMFLNGESFGQGRSSVKEILAKLDTGAGARAAKSLENKPVFDTLIVGGGPAGAAAAIYSARKGIATGV
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDIMDVQRAEALIPGDVHQIRLANGAVLKARTVVLATGA
RWREINVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVKAVTLIEYGAQLRADEVLQRKLRSLP
NVTVITEAQTTELTGDGSKLNGLVYTDRRSGEVKRIELEGVFVQIGLVPNTEWLKGTVELSKHGEIVVDARGATSVPGVF
AAGDVTTVPFKQIVIAVGEGAKASLGAFDHLIRQDATPAVDTQPQADEAVAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=64.6034816247582, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=32.8025477707006, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321898, Length=315, Percent_Identity=31.7460317460317, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320560, Length=325, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56980; Mature: 56980

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKTQLKAYLEKITRPIELVASLDDSAKSQELRALLDEIASLTTRVTVIERRDDEA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCC
RTPSFSIGEPGQPARIRFAGIPMGHEFTSLVLALLQTGGHPVKLDDDVIEQIRALDGDYA
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCEE
FETYFSLSCQNCPEVVQALNVMSLINPRIRHVAIDGALFQDEVEARQIMAVPTMFLNGES
EEHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHECCEEEECCCC
FGQGRSSVKEILAKLDTGAGARAAKSLENKPVFDTLIVGGGPAGAAAAIYSARKGIATGV
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHH
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDIMDVQRAEALIPGDVHQ
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCEE
IRLANGAVLKARTVVLATGARWREINVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEEEEEEECCCCEEEECCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGIVKAVTLIEYGAQLRADEVLQRKLRSLPNVTVITEAQTTELTGDGSKL
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCC
NGLVYTDRRSGEVKRIELEGVFVQIGLVPNTEWLKGTVELSKHGEIVVDARGATSVPGVF
CCEEEECCCCCCEEEEEEEEEEEEEEECCCHHHHEEEEEECCCCCEEEECCCCCCCCCEE
AAGDVTTVPFKQIVIAVGEGAKASLGAFDHLIRQDATPAVDTQPQADEAVAA
ECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MLDANLKTQLKAYLEKITRPIELVASLDDSAKSQELRALLDEIASLTTRVTVIERRDDEA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCC
RTPSFSIGEPGQPARIRFAGIPMGHEFTSLVLALLQTGGHPVKLDDDVIEQIRALDGDYA
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCEE
FETYFSLSCQNCPEVVQALNVMSLINPRIRHVAIDGALFQDEVEARQIMAVPTMFLNGES
EEHEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHECCEEEECCCC
FGQGRSSVKEILAKLDTGAGARAAKSLENKPVFDTLIVGGGPAGAAAAIYSARKGIATGV
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHH
VAERFGGQVLDTMAIENFVSVQETEGPKFATALEQHVKQYDVDIMDVQRAEALIPGDVHQ
HHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCEE
IRLANGAVLKARTVVLATGARWREINVPGEREYRNRGVAYCPHCDGPLFKGKRVAVVGGG
EEECCCCEEEEEEEEEECCCCEEEECCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGIVKAVTLIEYGAQLRADEVLQRKLRSLPNVTVITEAQTTELTGDGSKL
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCC
NGLVYTDRRSGEVKRIELEGVFVQIGLVPNTEWLKGTVELSKHGEIVVDARGATSVPGVF
CCEEEECCCCCCEEEEEEEEEEEEEEECCCHHHHEEEEEECCCCCEEEECCCCCCCCCEE
AAGDVTTVPFKQIVIAVGEGAKASLGAFDHLIRQDATPAVDTQPQADEAVAA
ECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]