Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 189352737

GI number: 189352737

Start: 842828

End: 844147

Strand: Reverse

Name: shiA [H]

Synonym: BMULJ_03969

Alternate gene names: 189352737

Gene position: 844147-842828 (Counterclockwise)

Preceding gene: 189352739

Following gene: 189352736

Centisome position: 34.13

GC content: 68.86

Gene sequence:

>1320_bases
ATGCAGACGCGATCCACGCTCGACGAGCATGCAACCGCCGCGCTGCCGGCCCCCGCGCGCACCGCGCGGCATCACCTGCT
GGCCGGCTGGGCCAGCATGGCCGGCACGACGATCGAGTGGTACGACTTCTTCCTCTACGGCACCGCCGCCGCGCTCGTGT
TCAACCGCATCTTCTTTCCGTCGCTCGACCCGGTCGTCGGCACGCTCGCCGCGTTCGGCACGTTCGCGGTCGGCTTCATC
GGGCGGCCGATGGGCGGCATCGTGTTCGGCCACTTCGGCGACCGCATCGGCCGCAAGTCGATGCTGATGATCACGCTGCT
GCTGATGGGCGTGCCGAGCATGATCATCGGGCTGATCCCGTCGTACGACCGCATCGGCTACTGGGCCGCCGCGCTGCTGA
TCGCGATGCGCTTCCTGCAGGGGATGGCGGTCGGCGGTGAATGGGGCGGCGCAGTGCTGATGGCCGTCGAGCATGCGCCG
AAGGGCCGCAAGGGGTTGTTCGGCAGCCTGCCGCAGACGGGCGTCGGGCTCGGCTTGATCCTCTCGTCGCTCGCGATGGC
GGCGGTCGCCGCGCTGCCGGAAGCCGACATGCTGTCGTGGGGCTGGCGCGTGCCGTTCCTCGCGAGCATCGCGCTGGTCG
GCCTCGGCTGGTTCATCCGCGCGAAGGTGCCGGAGTCGCCCGACTTCGAGAAGATGCAGCGCCAGGGCAAGGCCGAGAAG
TCGCCGGTGACGGCCGCGCTGCGCCGCCATCCGCGTGAAGTGCTGACGATCGTCGGCGCGCGTGCGGCCGAGAACACCTG
GTTCTACATGGTCGTCACGTTCGCGCTGTCGTATGCGACGCAGCAGCTCCATCTGCCGAAGGCCGAGATGCTGCACGCGA
TCACGGCCGGCGCGCTGCTGTCGCTCGTCACGATGCCGCTGTGCGGCCATCTGAGCGACCTGATCGGCCAGCGCCGGATG
TTCGCGATCGGGCTCGTGCTGATGTGCGTGTTCGCCGCACCGTTCTTCATGATGCTCGGCACGCAGCAGACCGCGCTCGC
ATGGTGGGCGATCGTGCTCGGCCTCGGCGTCGTGTTTCCGATTCTGTACGCACCCGAATCGCTGCTGTTCGCGCAGCAGT
TCCCCGCCGAAATCCGCTACAGCGGCATCTCGCTGTCGGTCCAGCTCGCCGGCGTGATCGGCGGCGGCTTCGCGCCGATG
ATCGCGACCTCGCTGCTCAAGGCCGGCGGCGGTCAGCCGCACTACGTCATCGCGTATCTCGTCGGCTTCGGCGTGTTCGC
GCTCGTGTGCACGGCGCTGATGCGTCCCGCACGCCACTGA

Upstream 100 bases:

>100_bases
ACCGGTTTCTTTGTCGGTACATCGCGAATCTGCGCATAACTATACTCGACCGTGACCGGCGGACCATGAGCCGCCGGCAC
ACACCAAATATGGAGACACC

Downstream 100 bases:

>100_bases
CACGCCGGCCGCGCCGGCTTCCCTCCACGTTCCGCGTCCGCGCGCCACACTGCGCGCGGCGGACGCGGCCGCCCTGTTTC
TTCATCACCCCGTTCTTCCG

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MQTRSTLDEHATAALPAPARTARHHLLAGWASMAGTTIEWYDFFLYGTAAALVFNRIFFPSLDPVVGTLAAFGTFAVGFI
GRPMGGIVFGHFGDRIGRKSMLMITLLLMGVPSMIIGLIPSYDRIGYWAAALLIAMRFLQGMAVGGEWGGAVLMAVEHAP
KGRKGLFGSLPQTGVGLGLILSSLAMAAVAALPEADMLSWGWRVPFLASIALVGLGWFIRAKVPESPDFEKMQRQGKAEK
SPVTAALRRHPREVLTIVGARAAENTWFYMVVTFALSYATQQLHLPKAEMLHAITAGALLSLVTMPLCGHLSDLIGQRRM
FAIGLVLMCVFAAPFFMMLGTQQTALAWWAIVLGLGVVFPILYAPESLLFAQQFPAEIRYSGISLSVQLAGVIGGGFAPM
IATSLLKAGGGQPHYVIAYLVGFGVFALVCTALMRPARH

Sequences:

>Translated_439_residues
MQTRSTLDEHATAALPAPARTARHHLLAGWASMAGTTIEWYDFFLYGTAAALVFNRIFFPSLDPVVGTLAAFGTFAVGFI
GRPMGGIVFGHFGDRIGRKSMLMITLLLMGVPSMIIGLIPSYDRIGYWAAALLIAMRFLQGMAVGGEWGGAVLMAVEHAP
KGRKGLFGSLPQTGVGLGLILSSLAMAAVAALPEADMLSWGWRVPFLASIALVGLGWFIRAKVPESPDFEKMQRQGKAEK
SPVTAALRRHPREVLTIVGARAAENTWFYMVVTFALSYATQQLHLPKAEMLHAITAGALLSLVTMPLCGHLSDLIGQRRM
FAIGLVLMCVFAAPFFMMLGTQQTALAWWAIVLGLGVVFPILYAPESLLFAQQFPAEIRYSGISLSVQLAGVIGGGFAPM
IATSLLKAGGGQPHYVIAYLVGFGVFALVCTALMRPARH
>Mature_439_residues
MQTRSTLDEHATAALPAPARTARHHLLAGWASMAGTTIEWYDFFLYGTAAALVFNRIFFPSLDPVVGTLAAFGTFAVGFI
GRPMGGIVFGHFGDRIGRKSMLMITLLLMGVPSMIIGLIPSYDRIGYWAAALLIAMRFLQGMAVGGEWGGAVLMAVEHAP
KGRKGLFGSLPQTGVGLGLILSSLAMAAVAALPEADMLSWGWRVPFLASIALVGLGWFIRAKVPESPDFEKMQRQGKAEK
SPVTAALRRHPREVLTIVGARAAENTWFYMVVTFALSYATQQLHLPKAEMLHAITAGALLSLVTMPLCGHLSDLIGQRRM
FAIGLVLMCVFAAPFFMMLGTQQTALAWWAIVLGLGVVFPILYAPESLLFAQQFPAEIRYSGISLSVQLAGVIGGGFAPM
IATSLLKAGGGQPHYVIAYLVGFGVFALVCTALMRPARH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=439, Percent_Identity=41.4578587699317, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1789941, Length=444, Percent_Identity=37.1621621621622, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790550, Length=417, Percent_Identity=32.6139088729017, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788942, Length=406, Percent_Identity=30.5418719211823, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082404, Length=345, Percent_Identity=24.9275362318841, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082231, Length=202, Percent_Identity=26.2376237623762, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47099; Mature: 47099

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRSTLDEHATAALPAPARTARHHLLAGWASMAGTTIEWYDFFLYGTAAALVFNRIFFP
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
SLDPVVGTLAAFGTFAVGFIGRPMGGIVFGHFGDRIGRKSMLMITLLLMGVPSMIIGLIP
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
SYDRIGYWAAALLIAMRFLQGMAVGGEWGGAVLMAVEHAPKGRKGLFGSLPQTGVGLGLI
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHH
LSSLAMAAVAALPEADMLSWGWRVPFLASIALVGLGWFIRAKVPESPDFEKMQRQGKAEK
HHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCCC
SPVTAALRRHPREVLTIVGARAAENTWFYMVVTFALSYATQQLHLPKAEMLHAITAGALL
CHHHHHHHHCHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SLVTMPLCGHLSDLIGQRRMFAIGLVLMCVFAAPFFMMLGTQQTALAWWAIVLGLGVVFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILYAPESLLFAQQFPAEIRYSGISLSVQLAGVIGGGFAPMIATSLLKAGGGQPHYVIAYL
HHHCCHHHHHHHHCCHHHEECCEEEEEEEEEHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
VGFGVFALVCTALMRPARH
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQTRSTLDEHATAALPAPARTARHHLLAGWASMAGTTIEWYDFFLYGTAAALVFNRIFFP
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
SLDPVVGTLAAFGTFAVGFIGRPMGGIVFGHFGDRIGRKSMLMITLLLMGVPSMIIGLIP
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHCC
SYDRIGYWAAALLIAMRFLQGMAVGGEWGGAVLMAVEHAPKGRKGLFGSLPQTGVGLGLI
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHH
LSSLAMAAVAALPEADMLSWGWRVPFLASIALVGLGWFIRAKVPESPDFEKMQRQGKAEK
HHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCCCCCC
SPVTAALRRHPREVLTIVGARAAENTWFYMVVTFALSYATQQLHLPKAEMLHAITAGALL
CHHHHHHHHCHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SLVTMPLCGHLSDLIGQRRMFAIGLVLMCVFAAPFFMMLGTQQTALAWWAIVLGLGVVFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ILYAPESLLFAQQFPAEIRYSGISLSVQLAGVIGGGFAPMIATSLLKAGGGQPHYVIAYL
HHHCCHHHHHHHHCCHHHEECCEEEEEEEEEHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
VGFGVFALVCTALMRPARH
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]