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The map label for this gene is folC [H]

Identifier: 189352662

GI number: 189352662

Start: 751550

End: 752860

Strand: Direct

Name: folC [H]

Synonym: BMULJ_03893

Alternate gene names: 189352662

Gene position: 751550-752860 (Clockwise)

Preceding gene: 189352661

Following gene: 189352663

Centisome position: 30.39

GC content: 66.51

Gene sequence:

>1311_bases
ATGAGCACTTTTCCCACCCTCGACGCGTGGCTCTCGCACCTCGAGCGCGCACACCCGGTCGGCATCGACATGGGCCTCAC
CCGCATCGGGCAGGTCAAGGCGGCGCTGCAACTCGAATTCGCGTGTCCGGTGATCACGGTCGGCGGCACGAACGGCAAGG
GCTCGACCTGCGCGTTCCTCGAGACGATCCTCGTGCGCGCGGGCTACAAGGTCGGCTGCCATACGTCGCCGCATCTGCTC
GAATTCAACGAACGCGCACGCGTGAACGGCGAGGTCGTCAGCGACGCGGAGTTGTTGCCGCATTTCGAGGCTGTCGAAGC
CGCGCGCACGTCGCTGCCCGAGCCGGTCTCGCTCACGTATTTCGAATTCACGACGCTCGCGATCCTGCATCTGTTCGCGT
CGCGCGGGCTCGACGCGGTGATCCTCGAAGTCGGCCTCGGCGGCCGGCTCGATGCGGTCAACATCATCGACACCGACTGC
GCGATCGTCACGAGCATCGACATCGACCACACCGAATATCTCGGCGACACGCGCGAGAAGATCGCGTTCGAGAAGGCCGG
AATTTTCCGTCCGGGCAAGCCGGCGATCTGCGGCGATCCCGCTGCGCCACAGACGCTCGTCGATCATGCGGCCGCGATCG
GCGCCGATCTGTGGCTCGTCGGCCGCGATTTCCGCTACGAGGCGCAGCCGGGCGCGGAGCGCCAGCAGTGGAGCTACATC
GGGCGCGACAAGCGCTATCCGGCGCTCGCGTATCCGGCGCTGCGCGGCGCGAATCAGCTGATCAATGCGTCCGCGGCGCT
GGCCGCGCTCGAGGCGTTGCGTCCGCAACTGCCGGTGTCCGCGCAGGACATCCGGCTCGGCCTCGCGAACGTCGAGCTGC
CCGGCCGCTTCCAGGTGCTGCCGGGCAAGCCGGCGATCGTGCTCGACGTCGCGCACAACCCGCATGCGGCGGCCGTGCTC
GAGCAGAACCTCGGCAACATGGGCTTCTTTCCGTACACGTACGCGGTGTTCGGCGCCATGCACGACAAGGACATCGACGG
CGTGCTGCGTCACCTGAAGGGCGAGATCGACCACTGGTGCGTGACCGATCTGCCGCTGCCGCGCGCGGCGAGTGCCGAGC
AGCTCGAAGCGGCGCTGCGCAAGGCCGGCGTGCAGGACGGGTCCGATTCGAGCGTCACGCGCTACGCGTCGCCGGCCGAT
GCGTTTAGCGATGCGCTAAAAAGAGCATCCGAGAATGATAGAATCGTGGTTTTCGGCAGTTTCCATACGGTTGCCGGCGT
GATGGCCTACCGTAAATCGCAGCAACACTGA

Upstream 100 bases:

>100_bases
CGCAGCGCCCGGCATGAACCGCGCGCGTCGCCCGACCGGTCCGGCGGGCGGCGCGCTTCGTTTTTCGGCGTAGTGACGGT
ACCGATCCAGATTTGATCCG

Downstream 100 bases:

>100_bases
CTGACGGGCAGTTTCGGACTCAGCCATTCATGGGAATTTTCTCGTTCGGCAAGAAAGACGACGACGCGCCCACCCGGCGC
GGCGGTCGTACCGGGGCCTC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTFPTLDAWLSHLERAHPVGIDMGLTRIGQVKAALQLEFACPVITVGGTNGKGSTCAFLETILVRAGYKVGCHTSPHLL
EFNERARVNGEVVSDAELLPHFEAVEAARTSLPEPVSLTYFEFTTLAILHLFASRGLDAVILEVGLGGRLDAVNIIDTDC
AIVTSIDIDHTEYLGDTREKIAFEKAGIFRPGKPAICGDPAAPQTLVDHAAAIGADLWLVGRDFRYEAQPGAERQQWSYI
GRDKRYPALAYPALRGANQLINASAALAALEALRPQLPVSAQDIRLGLANVELPGRFQVLPGKPAIVLDVAHNPHAAAVL
EQNLGNMGFFPYTYAVFGAMHDKDIDGVLRHLKGEIDHWCVTDLPLPRAASAEQLEAALRKAGVQDGSDSSVTRYASPAD
AFSDALKRASENDRIVVFGSFHTVAGVMAYRKSQQH

Sequences:

>Translated_436_residues
MSTFPTLDAWLSHLERAHPVGIDMGLTRIGQVKAALQLEFACPVITVGGTNGKGSTCAFLETILVRAGYKVGCHTSPHLL
EFNERARVNGEVVSDAELLPHFEAVEAARTSLPEPVSLTYFEFTTLAILHLFASRGLDAVILEVGLGGRLDAVNIIDTDC
AIVTSIDIDHTEYLGDTREKIAFEKAGIFRPGKPAICGDPAAPQTLVDHAAAIGADLWLVGRDFRYEAQPGAERQQWSYI
GRDKRYPALAYPALRGANQLINASAALAALEALRPQLPVSAQDIRLGLANVELPGRFQVLPGKPAIVLDVAHNPHAAAVL
EQNLGNMGFFPYTYAVFGAMHDKDIDGVLRHLKGEIDHWCVTDLPLPRAASAEQLEAALRKAGVQDGSDSSVTRYASPAD
AFSDALKRASENDRIVVFGSFHTVAGVMAYRKSQQH
>Mature_435_residues
STFPTLDAWLSHLERAHPVGIDMGLTRIGQVKAALQLEFACPVITVGGTNGKGSTCAFLETILVRAGYKVGCHTSPHLLE
FNERARVNGEVVSDAELLPHFEAVEAARTSLPEPVSLTYFEFTTLAILHLFASRGLDAVILEVGLGGRLDAVNIIDTDCA
IVTSIDIDHTEYLGDTREKIAFEKAGIFRPGKPAICGDPAAPQTLVDHAAAIGADLWLVGRDFRYEAQPGAERQQWSYIG
RDKRYPALAYPALRGANQLINASAALAALEALRPQLPVSAQDIRLGLANVELPGRFQVLPGKPAIVLDVAHNPHAAAVLE
QNLGNMGFFPYTYAVFGAMHDKDIDGVLRHLKGEIDHWCVTDLPLPRAASAEQLEAALRKAGVQDGSDSSVTRYASPADA
FSDALKRASENDRIVVFGSFHTVAGVMAYRKSQQH

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=310, Percent_Identity=36.4516129032258, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI66932990, Length=310, Percent_Identity=36.4516129032258, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788654, Length=423, Percent_Identity=43.9716312056738, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17553150, Length=294, Percent_Identity=31.9727891156463, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71984923, Length=294, Percent_Identity=31.9727891156463, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=295, Percent_Identity=32.2033898305085, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323760, Length=364, Percent_Identity=28.021978021978, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324815, Length=300, Percent_Identity=31, Blast_Score=116, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24641571, Length=162, Percent_Identity=40.1234567901235, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24581568, Length=168, Percent_Identity=36.3095238095238, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47061; Mature: 46930

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFPTLDAWLSHLERAHPVGIDMGLTRIGQVKAALQLEFACPVITVGGTNGKGSTCAFL
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEEECCEEEECCCCCCCCHHHHH
ETILVRAGYKVGCHTSPHLLEFNERARVNGEVVSDAELLPHFEAVEAARTSLPEPVSLTY
HHHHHHCCCEECCCCCCCEEECCCCCCCCCCEECCHHHCCCHHHHHHHHHCCCCCCEEEH
FEFTTLAILHLFASRGLDAVILEVGLGGRLDAVNIIDTDCAIVTSIDIDHTEYLGDTREK
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCEEEEEECCCHHHHHCCHHHH
IAFEKAGIFRPGKPAICGDPAAPQTLVDHAAAIGADLWLVGRDFRYEAQPGAERQQWSYI
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCEECCCCCCCHHHHHHC
GRDKRYPALAYPALRGANQLINASAALAALEALRPQLPVSAQDIRLGLANVELPGRFQVL
CCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCCCCCCEEEC
PGKPAIVLDVAHNPHAAAVLEQNLGNMGFFPYTYAVFGAMHDKDIDGVLRHLKGEIDHWC
CCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCEE
VTDLPLPRAASAEQLEAALRKAGVQDGSDSSVTRYASPADAFSDALKRASENDRIVVFGS
ECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCEEEEEEC
FHTVAGVMAYRKSQQH
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STFPTLDAWLSHLERAHPVGIDMGLTRIGQVKAALQLEFACPVITVGGTNGKGSTCAFL
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEEECCEEEECCCCCCCCHHHHH
ETILVRAGYKVGCHTSPHLLEFNERARVNGEVVSDAELLPHFEAVEAARTSLPEPVSLTY
HHHHHHCCCEECCCCCCCEEECCCCCCCCCCEECCHHHCCCHHHHHHHHHCCCCCCEEEH
FEFTTLAILHLFASRGLDAVILEVGLGGRLDAVNIIDTDCAIVTSIDIDHTEYLGDTREK
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCEEEEEECCCHHHHHCCHHHH
IAFEKAGIFRPGKPAICGDPAAPQTLVDHAAAIGADLWLVGRDFRYEAQPGAERQQWSYI
HHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCEECCCCCCCHHHHHHC
GRDKRYPALAYPALRGANQLINASAALAALEALRPQLPVSAQDIRLGLANVELPGRFQVL
CCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCCCCCCEEEC
PGKPAIVLDVAHNPHAAAVLEQNLGNMGFFPYTYAVFGAMHDKDIDGVLRHLKGEIDHWC
CCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCEE
VTDLPLPRAASAEQLEAALRKAGVQDGSDSSVTRYASPADAFSDALKRASENDRIVVFGS
ECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHCCCCCEEEEEEC
FHTVAGVMAYRKSQQH
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]