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The map label for this gene is phrB [H]

Identifier: 189352592

GI number: 189352592

Start: 673342

End: 674772

Strand: Reverse

Name: phrB [H]

Synonym: BMULJ_03823

Alternate gene names: 189352592

Gene position: 674772-673342 (Counterclockwise)

Preceding gene: 189352593

Following gene: 189352591

Centisome position: 27.28

GC content: 68.48

Gene sequence:

>1431_bases
ATGTCTGTCGCCATCGTGCTGTTTCGCCGCGACCTGCGGCTTTCCGACCATCCCGCGCTGTCGGCGGCATGCGCGCGGCA
CGATGCCGTGCTGCCGGTCTACGTACACACGCCGGATGAAGACGCGATCTGGTCGCCGGGTGCGGCGAGCCGCTGGTGGC
TGCACCACAGCCTGACGTCGCTCGATCGGGCACTGCGGGAGCAAGGCGGGCGATTGCTCGCGATGCAGAGCGACCCGACG
ATCGCCGTGCCGGCGCTCGCACGCGCGGTCGACGCACAGGGCGTGTACTGGACGCGCCGCTACGAACCGGCTGCCATCGC
GCGCGATGCGGCGCTGAAAAGCACGTTGCGCGCGCAAGGCATCGCCGCGCATAGCTACCCCGGCGCGCTCTGGCAGGAAC
CCTGGACGCTCGCGACCGACGCCGGGCAGCCCTATCGCGTCTTCACGCCTTTCTGGCGCAAGCTGCGCACGAGCTTGTCC
GACTGCCATCCGCTCCCGGCCGCACGACCTCGCCGATGGATGACGACGGACGATTCGACTGCCGTCGAGGCGCTCGGCCT
GTTGCCGCGCATTCGATGGGACGCGGGACTGGCCGACGCATGGCGCCCCGGAGAAGGAGGCGCGTGGGAACGCCTGGAAC
AGTTCGTCGACGAAGCGCTGGCCGAATACGGCGACGCGCGCGACGCGCCGGCTCGCCTGGGTACCTCGCGGCTTTCTGCG
CACTTGCATTACGGCGAGATCACGCCGACGCAGATTCTACGAACGCTGCAGGAACGCGTCGCGCGGACGAGCGGCAGCGT
CCGGCCCGACCTCGAGCCGTTTCTGCGGGAGCTCGGCTGGCGCGAGTTCGCGCATCATCTGCTCTACCACTTCCCGCACA
CGACCGACGCCAACTTCGATGCGCGCTTCGACGCCTTCGCGTGGGCGCCGGACGACGGCGAGCAGTTGGCGCGTTGGCAG
CAGGGGCGTACCGGCATTCCGCTCGTCGATGCCGGCATGCGGCAACTTTGGCAGACCGGCTGGATGCACAACCGCGTGAG
AATGGTGGTCGCGAGCTTTCTGACCAAGAACCTGCGTCAGCATTGGCGGCACGGCGCGCGATGGTTCTGGGACACGCTCG
TCGATGCGGACCTGGCGAACAACACGCTGGGCTGGCAATGGGTCGCCGGTTGCGGGGCCGATGCCGCGCCCTACTTCCGC
GTGTTCAATCCGTATCTGCAAGCCGCCAAGTTCGATCCCGACGCCGCGTATCTCAAACGCTGGCTCCCGGAACTGTCGCG
GCTGCCGGCCGACGTGCTGCATCGGCCATGGAAGCATTCGGCTGCGCTCGCAGCGTGCGGCTACCCTACGCCCATGGTCG
ACCTTGCGGCGTCGCGAGAAGCTGCGCTGGCGGCGTACGCGCAGCTTCCGAGGTCACATGGCGGCGCATGA

Upstream 100 bases:

>100_bases
TCCGATCAGCGCCTGCACCGAACGGGAAAGACGGCATCGTCAGCGAACCGCCGCCGCGCGACAACGCCTCGATGGCAGGC
ATGATGCGGGGCTGATCCGA

Downstream 100 bases:

>100_bases
CGACGCCCTCGCGGCCATCGCGCGGGCACTGCACGGGCAAACCCCGAGCGCGAAATCGCCGCCGGCCCGCGCGGCCGCTG
GTAGACTTTGGCAGCCTCCG

Product: deoxyribodipyrimidine photo lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSVAIVLFRRDLRLSDHPALSAACARHDAVLPVYVHTPDEDAIWSPGAASRWWLHHSLTSLDRALREQGGRLLAMQSDPT
IAVPALARAVDAQGVYWTRRYEPAAIARDAALKSTLRAQGIAAHSYPGALWQEPWTLATDAGQPYRVFTPFWRKLRTSLS
DCHPLPAARPRRWMTTDDSTAVEALGLLPRIRWDAGLADAWRPGEGGAWERLEQFVDEALAEYGDARDAPARLGTSRLSA
HLHYGEITPTQILRTLQERVARTSGSVRPDLEPFLRELGWREFAHHLLYHFPHTTDANFDARFDAFAWAPDDGEQLARWQ
QGRTGIPLVDAGMRQLWQTGWMHNRVRMVVASFLTKNLRQHWRHGARWFWDTLVDADLANNTLGWQWVAGCGADAAPYFR
VFNPYLQAAKFDPDAAYLKRWLPELSRLPADVLHRPWKHSAALAACGYPTPMVDLAASREAALAAYAQLPRSHGGA

Sequences:

>Translated_476_residues
MSVAIVLFRRDLRLSDHPALSAACARHDAVLPVYVHTPDEDAIWSPGAASRWWLHHSLTSLDRALREQGGRLLAMQSDPT
IAVPALARAVDAQGVYWTRRYEPAAIARDAALKSTLRAQGIAAHSYPGALWQEPWTLATDAGQPYRVFTPFWRKLRTSLS
DCHPLPAARPRRWMTTDDSTAVEALGLLPRIRWDAGLADAWRPGEGGAWERLEQFVDEALAEYGDARDAPARLGTSRLSA
HLHYGEITPTQILRTLQERVARTSGSVRPDLEPFLRELGWREFAHHLLYHFPHTTDANFDARFDAFAWAPDDGEQLARWQ
QGRTGIPLVDAGMRQLWQTGWMHNRVRMVVASFLTKNLRQHWRHGARWFWDTLVDADLANNTLGWQWVAGCGADAAPYFR
VFNPYLQAAKFDPDAAYLKRWLPELSRLPADVLHRPWKHSAALAACGYPTPMVDLAASREAALAAYAQLPRSHGGA
>Mature_475_residues
SVAIVLFRRDLRLSDHPALSAACARHDAVLPVYVHTPDEDAIWSPGAASRWWLHHSLTSLDRALREQGGRLLAMQSDPTI
AVPALARAVDAQGVYWTRRYEPAAIARDAALKSTLRAQGIAAHSYPGALWQEPWTLATDAGQPYRVFTPFWRKLRTSLSD
CHPLPAARPRRWMTTDDSTAVEALGLLPRIRWDAGLADAWRPGEGGAWERLEQFVDEALAEYGDARDAPARLGTSRLSAH
LHYGEITPTQILRTLQERVARTSGSVRPDLEPFLRELGWREFAHHLLYHFPHTTDANFDARFDAFAWAPDDGEQLARWQQ
GRTGIPLVDAGMRQLWQTGWMHNRVRMVVASFLTKNLRQHWRHGARWFWDTLVDADLANNTLGWQWVAGCGADAAPYFRV
FNPYLQAAKFDPDAAYLKRWLPELSRLPADVLHRPWKHSAALAACGYPTPMVDLAASREAALAAYAQLPRSHGGA

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=504, Percent_Identity=28.968253968254, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI188536100, Length=501, Percent_Identity=28.3433133732535, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI188536103, Length=462, Percent_Identity=27.0562770562771, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1786926, Length=481, Percent_Identity=37.6299376299376, Blast_Score=276, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6324962, Length=513, Percent_Identity=28.6549707602339, Blast_Score=192, Evalue=8e-50,
Organism=Drosophila melanogaster, GI17137248, Length=460, Percent_Identity=28.695652173913, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24585455, Length=460, Percent_Identity=28.695652173913, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24648152, Length=523, Percent_Identity=26.7686424474187, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53523; Mature: 53392

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAIVLFRRDLRLSDHPALSAACARHDAVLPVYVHTPDEDAIWSPGAASRWWLHHSLTS
CCEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
LDRALREQGGRLLAMQSDPTIAVPALARAVDAQGVYWTRRYEPAAIARDAALKSTLRAQG
HHHHHHHCCCEEEEECCCCCEEHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCC
IAAHSYPGALWQEPWTLATDAGQPYRVFTPFWRKLRTSLSDCHPLPAARPRRWMTTDDST
CCCCCCCCCHHCCCCEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
AVEALGLLPRIRWDAGLADAWRPGEGGAWERLEQFVDEALAEYGDARDAPARLGTSRLSA
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHH
HLHYGEITPTQILRTLQERVARTSGSVRPDLEPFLRELGWREFAHHLLYHFPHTTDANFD
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC
ARFDAFAWAPDDGEQLARWQQGRTGIPLVDAGMRQLWQTGWMHNRVRMVVASFLTKNLRQ
CCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
HWRHGARWFWDTLVDADLANNTLGWQWVAGCGADAAPYFRVFNPYLQAAKFDPDAAYLKR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
WLPELSRLPADVLHRPWKHSAALAACGYPTPMVDLAASREAALAAYAQLPRSHGGA
HHHHHHCCCHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHHHHHCHHHCCCC
>Mature Secondary Structure 
SVAIVLFRRDLRLSDHPALSAACARHDAVLPVYVHTPDEDAIWSPGAASRWWLHHSLTS
CEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
LDRALREQGGRLLAMQSDPTIAVPALARAVDAQGVYWTRRYEPAAIARDAALKSTLRAQG
HHHHHHHCCCEEEEECCCCCEEHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCC
IAAHSYPGALWQEPWTLATDAGQPYRVFTPFWRKLRTSLSDCHPLPAARPRRWMTTDDST
CCCCCCCCCHHCCCCEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
AVEALGLLPRIRWDAGLADAWRPGEGGAWERLEQFVDEALAEYGDARDAPARLGTSRLSA
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHH
HLHYGEITPTQILRTLQERVARTSGSVRPDLEPFLRELGWREFAHHLLYHFPHTTDANFD
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCC
ARFDAFAWAPDDGEQLARWQQGRTGIPLVDAGMRQLWQTGWMHNRVRMVVASFLTKNLRQ
CCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
HWRHGARWFWDTLVDADLANNTLGWQWVAGCGADAAPYFRVFNPYLQAAKFDPDAAYLKR
HHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
WLPELSRLPADVLHRPWKHSAALAACGYPTPMVDLAASREAALAAYAQLPRSHGGA
HHHHHHCCCHHHHCCCCCCCHHHEECCCCCCHHHHHHHHHHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]