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The map label for this gene is gatA [H]

Identifier: 189352537

GI number: 189352537

Start: 600963

End: 602336

Strand: Direct

Name: gatA [H]

Synonym: BMULJ_03765

Alternate gene names: 189352537

Gene position: 600963-602336 (Clockwise)

Preceding gene: 189352536

Following gene: 189352538

Centisome position: 24.3

GC content: 63.32

Gene sequence:

>1374_bases
ATGCGATCAATAAATACAGTAATCGGTCAGCTGCGTAGCGGCCGACTTCGATACGAAACGCTCCTGCAAGAAACACTGGA
TAAGGCTGCGACCGCCAAAAGCGCAGGCGACTGTTCATATGTCGAACTATTCGCTGCTCCAAAGCTACTCGAAGAAGCAC
GCGCATGGCAGGCGCGCGCTGACGCCGACCGGTCGCTTCCCGCCCTCGCAGGGCTTCCCGTGTCGGTGAAGGACCTGTTC
GACGTCGAGGGCAGCGTAACCGCGGCCGGCTCTGCACTGCTGCGCAGTGCGCCGCCCGCGAATGGCGATGCAGCCGCGGT
GCAGCGACTTCGCGAAGCCGGTGCGATTATCGTGGGCCGTACTAACATGAGCGAGTTCGCGTATTCTGGACTTGGCGTAA
ATCCGCACTACGGCACGCCGACGAATCCGGTCGGCGCTGGGCGCATCGCCGGCGGCTCCTCGTCTGGCGCAGCAGTCGCG
GTGGCGCGCGGCCTCTGCGCTGCGTCGCTCGCCACCGACACGGGCGGTTCAATACGCATTCCAGCGGCGCTGTGCGGGAT
CGCGGGGTTTAAGCCGACGGCGCAAGCGGTATCTCGTGATGGCGTGCTGCCCCTGTCGCGTTCACTGGACTCCGTCGGAC
CTATTGCCTCGACGGCCGAATGCTGCTCGATCGTCCACCAGGTGCTGACCGGCGGGGAGCCGGATACATCAACGCTTCGG
CTCGACACTTTGCGGCTCGCGGTCGTGACCGACTATCTGCTGGACGAACTCGATCCAATGGTCTCACGCGCGTTCGATCG
CGCGCTCGCCCTGCTGACTGCGGCTGGCGCCAGCGTCGAAACCGTAGCCTTTCCTGAACTCAATGCAATCCGCGCAGGCG
GTTACAACGCGAAGATCGTGGCCGCCGAAGCGTTCGCGTTTCATCGGAGCTGGCTCGCCTCGCACGCACATCTGTACGAC
CCCCGCGTGCATACGCGGCTGTTAGCGGGCAAGGATTTGCCGGCGGCGGACTATCTCGACGCGCTCGATGCCAGGGCAGA
ACTGATCAATGCGGCGCGGCGGCGTCTGGCGGCGTTCGATGCGTGGCTGATGCCGACAGTCGCCATAGTCGCGCCGAAAA
TCGGCGACCTAGACGATGACAACGCCTTTTTCCGCGCCAACGCTGCGATGTTGCGTAACCCTAGCGTTGTGAATTTGCTC
GATGGCTGTGCGCTCACATTGCCCATTCCTAGCGACAATCCGCTTCCACAGGGGTTGTCGGTATGCGGCCTTGGAGGCGC
GGATGCCGACGTCCTGGCTATAGGTCGCAGCATCGAAGCGGACTGGCGGCGAAATTCCCGCGGAACGGATCTTTGCGATC
CGATCGTCGCCTAG

Upstream 100 bases:

>100_bases
CGATCAAAATTTCTGCCTGGACTGGGGCCGCGGCGTCCGAGAATATGTTTCCAACGGTACTTGGTTATGGGCGGCCGCAG
CGCCGCTCTCTGGAATTAAC

Downstream 100 bases:

>100_bases
AAATCTACCTGAAATGAAAGGAGAAAGATCATGACACTGAGTTCTACCGTCACGGCCGTGAATAAGCCGGCCCAACTGCT
TGTGTGGACGGACATCGCTT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRSINTVIGQLRSGRLRYETLLQETLDKAATAKSAGDCSYVELFAAPKLLEEARAWQARADADRSLPALAGLPVSVKDLF
DVEGSVTAAGSALLRSAPPANGDAAAVQRLREAGAIIVGRTNMSEFAYSGLGVNPHYGTPTNPVGAGRIAGGSSSGAAVA
VARGLCAASLATDTGGSIRIPAALCGIAGFKPTAQAVSRDGVLPLSRSLDSVGPIASTAECCSIVHQVLTGGEPDTSTLR
LDTLRLAVVTDYLLDELDPMVSRAFDRALALLTAAGASVETVAFPELNAIRAGGYNAKIVAAEAFAFHRSWLASHAHLYD
PRVHTRLLAGKDLPAADYLDALDARAELINAARRRLAAFDAWLMPTVAIVAPKIGDLDDDNAFFRANAAMLRNPSVVNLL
DGCALTLPIPSDNPLPQGLSVCGLGGADADVLAIGRSIEADWRRNSRGTDLCDPIVA

Sequences:

>Translated_457_residues
MRSINTVIGQLRSGRLRYETLLQETLDKAATAKSAGDCSYVELFAAPKLLEEARAWQARADADRSLPALAGLPVSVKDLF
DVEGSVTAAGSALLRSAPPANGDAAAVQRLREAGAIIVGRTNMSEFAYSGLGVNPHYGTPTNPVGAGRIAGGSSSGAAVA
VARGLCAASLATDTGGSIRIPAALCGIAGFKPTAQAVSRDGVLPLSRSLDSVGPIASTAECCSIVHQVLTGGEPDTSTLR
LDTLRLAVVTDYLLDELDPMVSRAFDRALALLTAAGASVETVAFPELNAIRAGGYNAKIVAAEAFAFHRSWLASHAHLYD
PRVHTRLLAGKDLPAADYLDALDARAELINAARRRLAAFDAWLMPTVAIVAPKIGDLDDDNAFFRANAAMLRNPSVVNLL
DGCALTLPIPSDNPLPQGLSVCGLGGADADVLAIGRSIEADWRRNSRGTDLCDPIVA
>Mature_457_residues
MRSINTVIGQLRSGRLRYETLLQETLDKAATAKSAGDCSYVELFAAPKLLEEARAWQARADADRSLPALAGLPVSVKDLF
DVEGSVTAAGSALLRSAPPANGDAAAVQRLREAGAIIVGRTNMSEFAYSGLGVNPHYGTPTNPVGAGRIAGGSSSGAAVA
VARGLCAASLATDTGGSIRIPAALCGIAGFKPTAQAVSRDGVLPLSRSLDSVGPIASTAECCSIVHQVLTGGEPDTSTLR
LDTLRLAVVTDYLLDELDPMVSRAFDRALALLTAAGASVETVAFPELNAIRAGGYNAKIVAAEAFAFHRSWLASHAHLYD
PRVHTRLLAGKDLPAADYLDALDARAELINAARRRLAAFDAWLMPTVAIVAPKIGDLDDDNAFFRANAAMLRNPSVVNLL
DGCALTLPIPSDNPLPQGLSVCGLGGADADVLAIGRSIEADWRRNSRGTDLCDPIVA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=27.2, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI195972892, Length=251, Percent_Identity=30.6772908366534, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI166795287, Length=156, Percent_Identity=35.2564102564103, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=387, Percent_Identity=29.4573643410853, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71990152, Length=198, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=221, Percent_Identity=28.9592760180996, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=135, Percent_Identity=34.8148148148148, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=160, Percent_Identity=35, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=125, Percent_Identity=40.8, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=125, Percent_Identity=40.8, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=183, Percent_Identity=32.7868852459016, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=434, Percent_Identity=29.7235023041475, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323950, Length=429, Percent_Identity=25.6410256410256, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648113, Length=457, Percent_Identity=29.1028446389497, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21356731, Length=241, Percent_Identity=33.195020746888, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550774, Length=203, Percent_Identity=30.0492610837438, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648435, Length=203, Percent_Identity=30.0492610837438, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648437, Length=203, Percent_Identity=30.0492610837438, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648441, Length=147, Percent_Identity=36.0544217687075, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648439, Length=147, Percent_Identity=36.0544217687075, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI161078093, Length=238, Percent_Identity=33.6134453781513, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652985, Length=242, Percent_Identity=29.3388429752066, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922090, Length=242, Percent_Identity=29.3388429752066, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652981, Length=242, Percent_Identity=29.3388429752066, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652983, Length=242, Percent_Identity=29.3388429752066, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644968, Length=244, Percent_Identity=27.0491803278689, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47526; Mature: 47526

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSINTVIGQLRSGRLRYETLLQETLDKAATAKSAGDCSYVELFAAPKLLEEARAWQARA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DADRSLPALAGLPVSVKDLFDVEGSVTAAGSALLRSAPPANGDAAAVQRLREAGAIIVGR
CCCCCCCHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEC
TNMSEFAYSGLGVNPHYGTPTNPVGAGRIAGGSSSGAAVAVARGLCAASLATDTGGSIRI
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEC
PAALCGIAGFKPTAQAVSRDGVLPLSRSLDSVGPIASTAECCSIVHQVLTGGEPDTSTLR
HHHHHHHCCCCHHHHHHHCCCCCHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
LDTLRLAVVTDYLLDELDPMVSRAFDRALALLTAAGASVETVAFPELNAIRAGGYNAKIV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCEEEE
AAEAFAFHRSWLASHAHLYDPRVHTRLLAGKDLPAADYLDALDARAELINAARRRLAAFD
EHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLMPTVAIVAPKIGDLDDDNAFFRANAAMLRNPSVVNLLDGCALTLPIPSDNPLPQGLS
HHHHHHHHHHCCCCCCCCCCCCEEEECHHHHCCCCHHEECCCCEEEEECCCCCCCCCCCC
VCGLGGADADVLAIGRSIEADWRRNSRGTDLCDPIVA
EECCCCCCCHHEEECCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRSINTVIGQLRSGRLRYETLLQETLDKAATAKSAGDCSYVELFAAPKLLEEARAWQARA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DADRSLPALAGLPVSVKDLFDVEGSVTAAGSALLRSAPPANGDAAAVQRLREAGAIIVGR
CCCCCCCHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEC
TNMSEFAYSGLGVNPHYGTPTNPVGAGRIAGGSSSGAAVAVARGLCAASLATDTGGSIRI
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEC
PAALCGIAGFKPTAQAVSRDGVLPLSRSLDSVGPIASTAECCSIVHQVLTGGEPDTSTLR
HHHHHHHCCCCHHHHHHHCCCCCHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
LDTLRLAVVTDYLLDELDPMVSRAFDRALALLTAAGASVETVAFPELNAIRAGGYNAKIV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCEEEE
AAEAFAFHRSWLASHAHLYDPRVHTRLLAGKDLPAADYLDALDARAELINAARRRLAAFD
EHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLMPTVAIVAPKIGDLDDDNAFFRANAAMLRNPSVVNLLDGCALTLPIPSDNPLPQGLS
HHHHHHHHHHCCCCCCCCCCCCEEEECHHHHCCCCHHEECCCCEEEEECCCCCCCCCCCC
VCGLGGADADVLAIGRSIEADWRRNSRGTDLCDPIVA
EECCCCCCCHHEEECCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA