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The map label for this gene is livK [H]

Identifier: 189352531

GI number: 189352531

Start: 594773

End: 595921

Strand: Direct

Name: livK [H]

Synonym: BMULJ_03759

Alternate gene names: 189352531

Gene position: 594773-595921 (Clockwise)

Preceding gene: 189352530

Following gene: 189352532

Centisome position: 24.05

GC content: 56.83

Gene sequence:

>1149_bases
ATGAAATCCATCGAACTGAAGCGCACCTTGATCGGGGCTACAGCGTTGCTCGCGACATTCACCGTGTCAGTCGCGCATGC
GGACGACGTGATCGTCAAAATTGGCCATGTTGCACCGCTGACCGGGAGTGCTGCCTACCAAGGCAAGGATAGCGAAAATG
GAGCGCAGCTTGCGATCGACGAGATCAATCAGAAGGGACTCGTAATTGCTGGAAAGCACGTTCACCTGGTGCTCGACCCG
CAGGACGACGCGGGCGATCCGCGTCAAGCCACCCAAGTTGCACAGAAGCTTATTGATGACGGCGTAGTGGCGGTGGTCGG
GCATATGAATTCTGGCACGTCCATTCCAGCCGCAGCGGTCTATAGCGCGGCAGGCGTGCCTCAAATTTCGCCTGCGGCAA
CCAACCCCGATTACACCAGGCAGGGGTTCAAGACGACATTCCGACTTGTCGCTACTGACGCGAAGCAGGGGCCGGCACTG
GCTTCTTATGCGGTGAACAGTTTGCATGCGAAACGCTTTGCGATTGCAGACGATGCGACGGCATATGGCCAGGGGCTCGC
GACCGAGTTTGAGAAAACGGTAAAGGCGGCCGGCGGTGTGGTAGTGGCAAGGGAAGCGAGCAACGACAAAGCTATCGACT
TCAAGGCCATTCTTACGCGCATAAAGAGCGAGCGACCCGATGTCATCATGTACGGCGGCATGTACGCTACCGGTGGTCCG
CTGCTGAAGCAGGCGTCGGCCTTGGGCATCAAGAATCCGGTACTTGGTGGCGACGGGGTCTGCACAGAGCAGGTCGTTGA
GCTCGCTGGCGATGCGGTCAAGCAGTTGGTCTGCTCAGAGCCGGGATTGGCACTATCAAAAATGCCGAAGGGCGCGGTAT
TCGCGCAGCGCTACGAAGCTCGATTTCATGGGTCCGTGCAGCAACACGCACCATTCTCGTACGACGCTGTTTACGTGATC
GTAGACGCAATGAAGCGTGCAAATTCCGTCGAGGCTGCAAAGCTTCTCGCCGCGCTTCCTGCCACATCTTATGACGGTGT
GACGGGTCACATCGCATTTGATTCTCATGGGGACCTTCGTCAATCGAGTATCACCCTGTACGACTACAGAAGCAACAAGA
AGAGCGTACTTGACGCAGTTCAGATGTAA

Upstream 100 bases:

>100_bases
GGTCCGGACTTTTTCTATGCAACGAAGCGGCGATGGCCGTCCAGATCGCGGACTAAGCGAGGGGCAGCTGCGTCGGTAAC
AATCCCAAAGGAGACAACTA

Downstream 100 bases:

>100_bases
GTTTCCTGATCGCGAAACGCGCGCCTATCGTCGGCGCGCGCGCAAGAATCTCACAAAAAAGGCCATCGCGATTGCGCCGG
CCGTATCCACGCCAAGAGAT

Product: branched-chain amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKSIELKRTLIGATALLATFTVSVAHADDVIVKIGHVAPLTGSAAYQGKDSENGAQLAIDEINQKGLVIAGKHVHLVLDP
QDDAGDPRQATQVAQKLIDDGVVAVVGHMNSGTSIPAAAVYSAAGVPQISPAATNPDYTRQGFKTTFRLVATDAKQGPAL
ASYAVNSLHAKRFAIADDATAYGQGLATEFEKTVKAAGGVVVAREASNDKAIDFKAILTRIKSERPDVIMYGGMYATGGP
LLKQASALGIKNPVLGGDGVCTEQVVELAGDAVKQLVCSEPGLALSKMPKGAVFAQRYEARFHGSVQQHAPFSYDAVYVI
VDAMKRANSVEAAKLLAALPATSYDGVTGHIAFDSHGDLRQSSITLYDYRSNKKSVLDAVQM

Sequences:

>Translated_382_residues
MKSIELKRTLIGATALLATFTVSVAHADDVIVKIGHVAPLTGSAAYQGKDSENGAQLAIDEINQKGLVIAGKHVHLVLDP
QDDAGDPRQATQVAQKLIDDGVVAVVGHMNSGTSIPAAAVYSAAGVPQISPAATNPDYTRQGFKTTFRLVATDAKQGPAL
ASYAVNSLHAKRFAIADDATAYGQGLATEFEKTVKAAGGVVVAREASNDKAIDFKAILTRIKSERPDVIMYGGMYATGGP
LLKQASALGIKNPVLGGDGVCTEQVVELAGDAVKQLVCSEPGLALSKMPKGAVFAQRYEARFHGSVQQHAPFSYDAVYVI
VDAMKRANSVEAAKLLAALPATSYDGVTGHIAFDSHGDLRQSSITLYDYRSNKKSVLDAVQM
>Mature_382_residues
MKSIELKRTLIGATALLATFTVSVAHADDVIVKIGHVAPLTGSAAYQGKDSENGAQLAIDEINQKGLVIAGKHVHLVLDP
QDDAGDPRQATQVAQKLIDDGVVAVVGHMNSGTSIPAAAVYSAAGVPQISPAATNPDYTRQGFKTTFRLVATDAKQGPAL
ASYAVNSLHAKRFAIADDATAYGQGLATEFEKTVKAAGGVVVAREASNDKAIDFKAILTRIKSERPDVIMYGGMYATGGP
LLKQASALGIKNPVLGGDGVCTEQVVELAGDAVKQLVCSEPGLALSKMPKGAVFAQRYEARFHGSVQQHAPFSYDAVYVI
VDAMKRANSVEAAKLLAALPATSYDGVTGHIAFDSHGDLRQSSITLYDYRSNKKSVLDAVQM

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=369, Percent_Identity=32.520325203252, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI48994941, Length=370, Percent_Identity=30.2702702702703, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40063; Mature: 40063

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIELKRTLIGATALLATFTVSVAHADDVIVKIGHVAPLTGSAAYQGKDSENGAQLAID
CCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
EINQKGLVIAGKHVHLVLDPQDDAGDPRQATQVAQKLIDDGVVAVVGHMNSGTSIPAAAV
CCCCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHH
YSAAGVPQISPAATNPDYTRQGFKTTFRLVATDAKQGPALASYAVNSLHAKRFAIADDAT
HHHCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEEECCCCH
AYGQGLATEFEKTVKAAGGVVVAREASNDKAIDFKAILTRIKSERPDVIMYGGMYATGGP
HHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCEEECCCH
LLKQASALGIKNPVLGGDGVCTEQVVELAGDAVKQLVCSEPGLALSKMPKGAVFAQRYEA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHH
RFHGSVQQHAPFSYDAVYVIVDAMKRANSVEAAKLLAALPATSYDGVTGHIAFDSHGDLR
HHCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
QSSITLYDYRSNKKSVLDAVQM
CCEEEEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MKSIELKRTLIGATALLATFTVSVAHADDVIVKIGHVAPLTGSAAYQGKDSENGAQLAID
CCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
EINQKGLVIAGKHVHLVLDPQDDAGDPRQATQVAQKLIDDGVVAVVGHMNSGTSIPAAAV
CCCCCCEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHH
YSAAGVPQISPAATNPDYTRQGFKTTFRLVATDAKQGPALASYAVNSLHAKRFAIADDAT
HHHCCCCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEEECCCCH
AYGQGLATEFEKTVKAAGGVVVAREASNDKAIDFKAILTRIKSERPDVIMYGGMYATGGP
HHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCEEECCCH
LLKQASALGIKNPVLGGDGVCTEQVVELAGDAVKQLVCSEPGLALSKMPKGAVFAQRYEA
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHH
RFHGSVQQHAPFSYDAVYVIVDAMKRANSVEAAKLLAALPATSYDGVTGHIAFDSHGDLR
HHCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
QSSITLYDYRSNKKSVLDAVQM
CCEEEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]