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The map label for this gene is ilvB [H]

Identifier: 189352513

GI number: 189352513

Start: 569609

End: 571423

Strand: Reverse

Name: ilvB [H]

Synonym: BMULJ_03736

Alternate gene names: 189352513

Gene position: 571423-569609 (Counterclockwise)

Preceding gene: 189352514

Following gene: 189352512

Centisome position: 23.1

GC content: 60.66

Gene sequence:

>1815_bases
ATGAACTCACTCGCTGAAGATCAGGCAGCCAATGCCCACCAAACTGAAGGCACGGTCGCAGAGGATCGCGAAAATTACTG
GGGAAGTGACGGAATCGCGGAACTGCTCCAGAGTCTGGACATACCATTCCTGTGCCTGAATCCGGGCTCGAGTTTCCGGG
GATTGCATGACAGCATTGTCAACCACACGGGCAACACCAACCCTGAATTCCTTCTTTGCCTGCATGAGGAACACGCCGTG
GCCATCGCTCACGGTTACGCGAAGGTGAAGGGTTCGCCGCTTGCCGTCGCCCTGCACAGCAACGTTGGGCTGATGCACGC
GAGCATGGCAATCTATAACGCCTGGTGTGACCGCGTCCCGATGCTGGTACTTGGTGCCCATGGTCCGGCCGATGCGGCGA
AGCGCAGACCATGGATCGACTGGATCCACACGTCGCAGGACACGGCTGCACTAGTGCGACCGTTCGTCAAATGGGACAAC
CAGCCCCTCTCCGTCGATGCGGCGTACGAGGCGATACTCCGTGCGTTCCAGATCACCCGAACGCAACCGAGCGCGCCGGT
CTATGTCTGTCTGGATGTGACATTTCAGGAAACGCGGCTCGAGGGACGACCACGCATCCCTGACGCGGCTCGCTACGCGC
CCCCCGCCCCGCCCTATCCGCGTCCGGCCGACATCGACCAGGCGGTGCAGGTGCTTTCCCACGGAAAGCGCCCCGTCATG
CTGATTGGCCGGGTTTCGAGAGACACCGTCGAATGGCGCGAACGGATCGAACTGGCCGAGAGGCTCGGAGCCACTGTACT
GACCGATCTGAAAACTGCTGCCAGTTTCCCAACGGACCATCCTCAACATCCACATCCGCCCGCATTCTTCGTCAACGGCC
CGTCCGCGGAAGCGCTGCGTCAGGCCGATGTGGTCCTCGCTCTCGACTGGGTCGATCTGGCTGGAACTTTAAAGGCTGCA
TGGCCCCAGAGTCCCGTCAGCGCACACGTCATTTCCGTATCGCTGGATCACACGCTCCACAACGGATGGAGCTTCGATCA
TCAAGGTCTGCCGGCAGTCGACATTGCGATCGCCTCGGACACAGGTATCACTGTCCGGGAACTGCTTGCCGCATTGCCGC
AGGATCGTCAGCTCAACAACGAGGTATCTTCACGCGTACGCCCTGCCCGGCTACCAGCCGAAGGTTCTGGCATTTCCATG
GAAACGCTGGCGTCGACTCTGCGCGCAGCCACCCGTAGCATGGACGTCTCACTCCTCCGACTCCCTCTGGGCTGGAGCGG
GGAGCTATGGGATTTTCATTCGCCCCTCGACTATCTCGGCTATGACGGCGGCGCCGGGATAGGATCCGGTCCCGGCATGG
CTGTCGGTGCCGCGCTAGCACTGGCAGGCACAGGACGACTCCCAGTTGCAATCCTGGGAGACGGTGACTTCCTGATGGGC
AACACTGCTCTATGGTCCGCCGCGCACTTTGACGTTCCGCTCCTGGTTATTGTCGCAAACAACCAGTCGTTCTATAACGA
CGAAGTCCACCAGGAGCGCGTTGCGCTCGAACGAGGTCGTCCCGTCGAAAACAAATGGATCGGCCAGCGTATCAGTTCGC
CTCTGGTAGACCTTGCAGCCATGGCGAGGGCCCAGGGGTTCACCGCAACAGGACCAGTCGTCGACAGCAATGCCCTGTCC
GTCGCACTCGCTGAAGCCATAAGCAAAGTTCTTGAAGGCGGCCAGGTGCTTGTCGATGTTCATGTGAAGCCGGGCTATGC
GCCTTCCATGAGCCGGGGCATGACCGCCTCAGCAACAGACAAGGCAACGCGCTGA

Upstream 100 bases:

>100_bases
GACTCGGCCGCGAGGAATGTCTTGAAGAACTGCTGGATTTCACGGCGACCAAGAGCATCAACATACAACTTTGACAGAAT
GACAGGGCGTCCAAACAGAG

Downstream 100 bases:

>100_bases
CGTCCTTCCCGCCCTGAAAGACCTCAGGGCGGGAATTCCCTACAAGAAAGCTACGTTTGACCGCGAAGCCCGGACAGTGT
CGGGTCACCCTGCACTACCG

Product: thiamine pyrophosphate-requiring pyruvate decarboxylase

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MNSLAEDQAANAHQTEGTVAEDRENYWGSDGIAELLQSLDIPFLCLNPGSSFRGLHDSIVNHTGNTNPEFLLCLHEEHAV
AIAHGYAKVKGSPLAVALHSNVGLMHASMAIYNAWCDRVPMLVLGAHGPADAAKRRPWIDWIHTSQDTAALVRPFVKWDN
QPLSVDAAYEAILRAFQITRTQPSAPVYVCLDVTFQETRLEGRPRIPDAARYAPPAPPYPRPADIDQAVQVLSHGKRPVM
LIGRVSRDTVEWRERIELAERLGATVLTDLKTAASFPTDHPQHPHPPAFFVNGPSAEALRQADVVLALDWVDLAGTLKAA
WPQSPVSAHVISVSLDHTLHNGWSFDHQGLPAVDIAIASDTGITVRELLAALPQDRQLNNEVSSRVRPARLPAEGSGISM
ETLASTLRAATRSMDVSLLRLPLGWSGELWDFHSPLDYLGYDGGAGIGSGPGMAVGAALALAGTGRLPVAILGDGDFLMG
NTALWSAAHFDVPLLVIVANNQSFYNDEVHQERVALERGRPVENKWIGQRISSPLVDLAAMARAQGFTATGPVVDSNALS
VALAEAISKVLEGGQVLVDVHVKPGYAPSMSRGMTASATDKATR

Sequences:

>Translated_604_residues
MNSLAEDQAANAHQTEGTVAEDRENYWGSDGIAELLQSLDIPFLCLNPGSSFRGLHDSIVNHTGNTNPEFLLCLHEEHAV
AIAHGYAKVKGSPLAVALHSNVGLMHASMAIYNAWCDRVPMLVLGAHGPADAAKRRPWIDWIHTSQDTAALVRPFVKWDN
QPLSVDAAYEAILRAFQITRTQPSAPVYVCLDVTFQETRLEGRPRIPDAARYAPPAPPYPRPADIDQAVQVLSHGKRPVM
LIGRVSRDTVEWRERIELAERLGATVLTDLKTAASFPTDHPQHPHPPAFFVNGPSAEALRQADVVLALDWVDLAGTLKAA
WPQSPVSAHVISVSLDHTLHNGWSFDHQGLPAVDIAIASDTGITVRELLAALPQDRQLNNEVSSRVRPARLPAEGSGISM
ETLASTLRAATRSMDVSLLRLPLGWSGELWDFHSPLDYLGYDGGAGIGSGPGMAVGAALALAGTGRLPVAILGDGDFLMG
NTALWSAAHFDVPLLVIVANNQSFYNDEVHQERVALERGRPVENKWIGQRISSPLVDLAAMARAQGFTATGPVVDSNALS
VALAEAISKVLEGGQVLVDVHVKPGYAPSMSRGMTASATDKATR
>Mature_604_residues
MNSLAEDQAANAHQTEGTVAEDRENYWGSDGIAELLQSLDIPFLCLNPGSSFRGLHDSIVNHTGNTNPEFLLCLHEEHAV
AIAHGYAKVKGSPLAVALHSNVGLMHASMAIYNAWCDRVPMLVLGAHGPADAAKRRPWIDWIHTSQDTAALVRPFVKWDN
QPLSVDAAYEAILRAFQITRTQPSAPVYVCLDVTFQETRLEGRPRIPDAARYAPPAPPYPRPADIDQAVQVLSHGKRPVM
LIGRVSRDTVEWRERIELAERLGATVLTDLKTAASFPTDHPQHPHPPAFFVNGPSAEALRQADVVLALDWVDLAGTLKAA
WPQSPVSAHVISVSLDHTLHNGWSFDHQGLPAVDIAIASDTGITVRELLAALPQDRQLNNEVSSRVRPARLPAEGSGISM
ETLASTLRAATRSMDVSLLRLPLGWSGELWDFHSPLDYLGYDGGAGIGSGPGMAVGAALALAGTGRLPVAILGDGDFLMG
NTALWSAAHFDVPLLVIVANNQSFYNDEVHQERVALERGRPVENKWIGQRISSPLVDLAAMARAQGFTATGPVVDSNALS
VALAEAISKVLEGGQVLVDVHVKPGYAPSMSRGMTASATDKATR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=506, Percent_Identity=22.3320158102767, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320816, Length=518, Percent_Identity=22.3938223938224, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64935; Mature: 64935

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLAEDQAANAHQTEGTVAEDRENYWGSDGIAELLQSLDIPFLCLNPGSSFRGLHDSIV
CCCCCCHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
NHTGNTNPEFLLCLHEEHAVAIAHGYAKVKGSPLAVALHSNVGLMHASMAIYNAWCDRVP
HCCCCCCCCEEEEEECCCEEEEEECHHEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
MLVLGAHGPADAAKRRPWIDWIHTSQDTAALVRPFVKWDNQPLSVDAAYEAILRAFQITR
EEEEECCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
TQPSAPVYVCLDVTFQETRLEGRPRIPDAARYAPPAPPYPRPADIDQAVQVLSHGKRPVM
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
LIGRVSRDTVEWRERIELAERLGATVLTDLKTAASFPTDHPQHPHPPAFFVNGPSAEALR
EEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHH
QADVVLALDWVDLAGTLKAAWPQSPVSAHVISVSLDHTLHNGWSFDHQGLPAVDIAIASD
HCCEEEEEECHHHHCHHHHCCCCCCCCEEEEEEEECHHHCCCCCCCCCCCCEEEEEEECC
TGITVRELLAALPQDRQLNNEVSSRVRPARLPAEGSGISMETLASTLRAATRSMDVSLLR
CCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEE
LPLGWSGELWDFHSPLDYLGYDGGAGIGSGPGMAVGAALALAGTGRLPVAILGDGDFLMG
ECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCEEEC
NTALWSAAHFDVPLLVIVANNQSFYNDEVHQERVALERGRPVENKWIGQRISSPLVDLAA
CHHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHH
MARAQGFTATGPVVDSNALSVALAEAISKVLEGGQVLVDVHVKPGYAPSMSRGMTASATD
HHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCCCC
KATR
CCCC
>Mature Secondary Structure
MNSLAEDQAANAHQTEGTVAEDRENYWGSDGIAELLQSLDIPFLCLNPGSSFRGLHDSIV
CCCCCCHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
NHTGNTNPEFLLCLHEEHAVAIAHGYAKVKGSPLAVALHSNVGLMHASMAIYNAWCDRVP
HCCCCCCCCEEEEEECCCEEEEEECHHEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
MLVLGAHGPADAAKRRPWIDWIHTSQDTAALVRPFVKWDNQPLSVDAAYEAILRAFQITR
EEEEECCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
TQPSAPVYVCLDVTFQETRLEGRPRIPDAARYAPPAPPYPRPADIDQAVQVLSHGKRPVM
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
LIGRVSRDTVEWRERIELAERLGATVLTDLKTAASFPTDHPQHPHPPAFFVNGPSAEALR
EEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHH
QADVVLALDWVDLAGTLKAAWPQSPVSAHVISVSLDHTLHNGWSFDHQGLPAVDIAIASD
HCCEEEEEECHHHHCHHHHCCCCCCCCEEEEEEEECHHHCCCCCCCCCCCCEEEEEEECC
TGITVRELLAALPQDRQLNNEVSSRVRPARLPAEGSGISMETLASTLRAATRSMDVSLLR
CCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEE
LPLGWSGELWDFHSPLDYLGYDGGAGIGSGPGMAVGAALALAGTGRLPVAILGDGDFLMG
ECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCEEEC
NTALWSAAHFDVPLLVIVANNQSFYNDEVHQERVALERGRPVENKWIGQRISSPLVDLAA
CHHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHH
MARAQGFTATGPVVDSNALSVALAEAISKVLEGGQVLVDVHVKPGYAPSMSRGMTASATD
HHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCCCC
KATR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]