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The map label for this gene is ysgA [C]

Identifier: 189352472

GI number: 189352472

Start: 522205

End: 523080

Strand: Direct

Name: ysgA [C]

Synonym: BMULJ_03691

Alternate gene names: 189352472

Gene position: 522205-523080 (Clockwise)

Preceding gene: 189352471

Following gene: 189352475

Centisome position: 21.11

GC content: 65.98

Gene sequence:

>876_bases
ATGTTGAAACCCGAAGTCGACAGCCTGGTTCCCCACGTCCCGTTCTCGCGCCGCAAGTTCATGCAGGCGGCGCTGGGCGG
CACTTTCGCGGCGGCCGTGCTGCCGGTGTCCGCGCAGACGATCACGACCGACAGCGCCGGCCTCGACGTCGACACCGTCG
AGATCCGCTCCGGTGACGCGAGCGTGCCGGCGTATCGCGCGCAGCCGGTCGACAAGACGAACCTGCCGGTCGTCATCGTG
ATCCACGAGATCTTCGGCGTGCATGCGCACATCGCCGACGTCTGCCGCCGCTTCGCGAAGCTCGGCTATCTCGCGATCGC
GCCCGACCTGTTTGCGCGGCAGGGCAACGCGTCGAAGTATTCGACGATCAAGGACCTGTACGACAACATCATCAACAAGG
TGCCCGACCGGCAGGTCACCGAGGATCTCGATGCGACGGTCGCCTGGGCGGGCAAGAACGGCGGCGACCTGACGCGCCTG
GGCGTGACGGGGTTCTGCTGGGGCGGCCGCCAGGCGTGGCTCTACGCGGAGCACAATCCGCACGTGCGGGCGGCCGTCGC
GTGGTACGGCTTCGTCGAAGGCAAGACCGACGAGATGACGCCGTTCAATCCGGTCGATCATGCGTCGTTGCTGAAGGTGC
CCGTGCTCGGGCTGTACGGCGAGAAGGATCAGAACATCACGCAGGCGTCGCTCGCCGACATGCGCAAGGCGATCCAGACG
AGCGACTCGAAGCTCGCGCGCGAGTCGGAGATCGTCGTCTATCCGGATGCCGGCCACGCGTTTTTTGCCGACTACCGGCC
GAGCTACGTGAAGGCCGATGCCGAGGACGGCTGGAAGCGCGCGATCGCGTGGTTCCACAAATACGGCGTGATGTAA

Upstream 100 bases:

>100_bases
GGCGTGCCACCCGCCGCAATCTTCGCACCGGGCGACGGTGCGATGCCGCGTCGCGTCGAATCCGTGGCGTGACGGCGGCG
CCCAATCGAGGAGTGCATTC

Downstream 100 bases:

>100_bases
ACGACGAAGCGGCGGCCGAGGCCGCCGCTTGGCTATCGCGCCGTACGCGCGTGCGTCACGGTGTCGCGCCGGTCGCGATC
GGCCGCGACGGATCGGCGCT

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLKPEVDSLVPHVPFSRRKFMQAALGGTFAAAVLPVSAQTITTDSAGLDVDTVEIRSGDASVPAYRAQPVDKTNLPVVIV
IHEIFGVHAHIADVCRRFAKLGYLAIAPDLFARQGNASKYSTIKDLYDNIINKVPDRQVTEDLDATVAWAGKNGGDLTRL
GVTGFCWGGRQAWLYAEHNPHVRAAVAWYGFVEGKTDEMTPFNPVDHASLLKVPVLGLYGEKDQNITQASLADMRKAIQT
SDSKLARESEIVVYPDAGHAFFADYRPSYVKADAEDGWKRAIAWFHKYGVM

Sequences:

>Translated_291_residues
MLKPEVDSLVPHVPFSRRKFMQAALGGTFAAAVLPVSAQTITTDSAGLDVDTVEIRSGDASVPAYRAQPVDKTNLPVVIV
IHEIFGVHAHIADVCRRFAKLGYLAIAPDLFARQGNASKYSTIKDLYDNIINKVPDRQVTEDLDATVAWAGKNGGDLTRL
GVTGFCWGGRQAWLYAEHNPHVRAAVAWYGFVEGKTDEMTPFNPVDHASLLKVPVLGLYGEKDQNITQASLADMRKAIQT
SDSKLARESEIVVYPDAGHAFFADYRPSYVKADAEDGWKRAIAWFHKYGVM
>Mature_291_residues
MLKPEVDSLVPHVPFSRRKFMQAALGGTFAAAVLPVSAQTITTDSAGLDVDTVEIRSGDASVPAYRAQPVDKTNLPVVIV
IHEIFGVHAHIADVCRRFAKLGYLAIAPDLFARQGNASKYSTIKDLYDNIINKVPDRQVTEDLDATVAWAGKNGGDLTRL
GVTGFCWGGRQAWLYAEHNPHVRAAVAWYGFVEGKTDEMTPFNPVDHASLLKVPVLGLYGEKDQNITQASLADMRKAIQT
SDSKLARESEIVVYPDAGHAFFADYRPSYVKADAEDGWKRAIAWFHKYGVM

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=262, Percent_Identity=50.381679389313, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 31964; Mature: 31964

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPEVDSLVPHVPFSRRKFMQAALGGTFAAAVLPVSAQTITTDSAGLDVDTVEIRSGDA
CCCCCHHHHCCCCCHHHHHHHHHHHCCHHHHHHEECCCEEEECCCCCCCEEEEEEECCCC
SVPAYRAQPVDKTNLPVVIVIHEIFGVHAHIADVCRRFAKLGYLAIAPDLFARQGNASKY
CCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCHHH
STIKDLYDNIINKVPDRQVTEDLDATVAWAGKNGGDLTRLGVTGFCWGGRQAWLYAEHNP
HHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCEEEECCEEEEECCCEEEEEECCCC
HVRAAVAWYGFVEGKTDEMTPFNPVDHASLLKVPVLGLYGEKDQNITQASLADMRKAIQT
CEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHC
SDSKLARESEIVVYPDAGHAFFADYRPSYVKADAEDGWKRAIAWFHKYGVM
CCHHHHCCCCEEEECCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKPEVDSLVPHVPFSRRKFMQAALGGTFAAAVLPVSAQTITTDSAGLDVDTVEIRSGDA
CCCCCHHHHCCCCCHHHHHHHHHHHCCHHHHHHEECCCEEEECCCCCCCEEEEEEECCCC
SVPAYRAQPVDKTNLPVVIVIHEIFGVHAHIADVCRRFAKLGYLAIAPDLFARQGNASKY
CCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCHHH
STIKDLYDNIINKVPDRQVTEDLDATVAWAGKNGGDLTRLGVTGFCWGGRQAWLYAEHNP
HHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCEEEECCEEEEECCCEEEEEECCCC
HVRAAVAWYGFVEGKTDEMTPFNPVDHASLLKVPVLGLYGEKDQNITQASLADMRKAIQT
CEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHC
SDSKLARESEIVVYPDAGHAFFADYRPSYVKADAEDGWKRAIAWFHKYGVM
CCHHHHCCCCEEEECCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168622 [H]