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The map label for this gene is insH10 [H]

Identifier: 189352433

GI number: 189352433

Start: 486558

End: 487550

Strand: Direct

Name: insH10 [H]

Synonym: BMULJ_03652

Alternate gene names: 189352433

Gene position: 486558-487550 (Clockwise)

Preceding gene: 189352432

Following gene: 189352462

Centisome position: 19.67

GC content: 62.24

Gene sequence:

>993_bases
ATGCAGCGGCAGATCGGTTTTGCGGAAGCGGAAAGTGCGGGCAAGAAGCGGGTGACCAAGCGTCAACGCTTTCTGGCCGA
GATGGAGAAGGTCGTCCCTTGGTCGCGGCTGCTGTCGGTGATCGGGCCGTATTACCCGAAGGGCGAGCGTGGCCGGCCGC
CGATTGGCCTGGAACGGATGCTGCGGATCTACTTGCTGCAGCAGTGGTACGGGTTGTCGGACGAAGGGCTTGAAGATGCG
CTCTACGACAGCATCGCGATGCGCGCCTTCGCGGGCATCGACCTGGCGCGCGAGAACGTGCCGGACGCCACCACGCTGTT
GAAATTCCGGCGCCTGCTGGTCGAGCACGCACTGACGCGAAAGCTGTTCGACGAGATCGGCATCGAGTTGTGCGAGCGCG
GGCTGATGATGAAGGAAGGCACGCTGGTGGATGCGACGATCTTCGAGGCTGCGCCGTCCACGAAGAACGCCGGGAAGAGC
CGTGACCCGGAGATGCATCAGACGAAGAAGGGCAACGACTGGTATTTCGGCATGAAGGCCCATGTCGGCGTCGACGCCGA
CTCGGGTCTGGTGCATAGCGTGGTCACCACGGCGGCCAACGAGCCGGACGTATCGCAGGCCCACGCCCTGCTGCACGGTC
ATGAGCAGGAAGCGTTTGGCGATGCGGGCTACACCGGCGTGGACAAGCGCGAGGAGATGAAGGGCAAGACGGTGAAGTGG
CACGTGGCGCTCAAGCGCGGAAAGATCAGGGCGATGCAGGAAGGTCCGCTGAAGGACCTCGTGATCGCGGTCGAGCGAAC
CAAGGCGCAGATCCGCGCCCGGGTCGAGCATCCGTTTCATGTCGTGAAGAACCTGTTTCGTCATCGCAAGGTGCGCTACA
AGGGTCTAGCCAGGAACACGGCACAGCTGTTCAGTCTGTTCGCGCTGGCGAACCTGGTGATCGCGAAAAATCAGCTGTTG
TCGACTCATGGGAGCAATCCGTCATGTGTGTGA

Upstream 100 bases:

>100_bases
CCGCGGTTCCTAAGGAAACACTGATCAATTCGGCTTGGCGGTCATCGCTGACGAGGCCAAGGGCGTTGTAGAAAGAAGCT
CACGGAACGGACCCACGACG

Downstream 100 bases:

>100_bases
AAAACGCGGGAAGTGAGGCCCGAATACGAGCGAAACTCACCTCGCATGTCGTCAAATTCCTACGTCAATCTGAAAATTGC
AACCTGCCTCGTCCGTTATG

Product: ISBmu23 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MQRQIGFAEAESAGKKRVTKRQRFLAEMEKVVPWSRLLSVIGPYYPKGERGRPPIGLERMLRIYLLQQWYGLSDEGLEDA
LYDSIAMRAFAGIDLARENVPDATTLLKFRRLLVEHALTRKLFDEIGIELCERGLMMKEGTLVDATIFEAAPSTKNAGKS
RDPEMHQTKKGNDWYFGMKAHVGVDADSGLVHSVVTTAANEPDVSQAHALLHGHEQEAFGDAGYTGVDKREEMKGKTVKW
HVALKRGKIRAMQEGPLKDLVIAVERTKAQIRARVEHPFHVVKNLFRHRKVRYKGLARNTAQLFSLFALANLVIAKNQLL
STHGSNPSCV

Sequences:

>Translated_330_residues
MQRQIGFAEAESAGKKRVTKRQRFLAEMEKVVPWSRLLSVIGPYYPKGERGRPPIGLERMLRIYLLQQWYGLSDEGLEDA
LYDSIAMRAFAGIDLARENVPDATTLLKFRRLLVEHALTRKLFDEIGIELCERGLMMKEGTLVDATIFEAAPSTKNAGKS
RDPEMHQTKKGNDWYFGMKAHVGVDADSGLVHSVVTTAANEPDVSQAHALLHGHEQEAFGDAGYTGVDKREEMKGKTVKW
HVALKRGKIRAMQEGPLKDLVIAVERTKAQIRARVEHPFHVVKNLFRHRKVRYKGLARNTAQLFSLFALANLVIAKNQLL
STHGSNPSCV
>Mature_330_residues
MQRQIGFAEAESAGKKRVTKRQRFLAEMEKVVPWSRLLSVIGPYYPKGERGRPPIGLERMLRIYLLQQWYGLSDEGLEDA
LYDSIAMRAFAGIDLARENVPDATTLLKFRRLLVEHALTRKLFDEIGIELCERGLMMKEGTLVDATIFEAAPSTKNAGKS
RDPEMHQTKKGNDWYFGMKAHVGVDADSGLVHSVVTTAANEPDVSQAHALLHGHEQEAFGDAGYTGVDKREEMKGKTVKW
HVALKRGKIRAMQEGPLKDLVIAVERTKAQIRARVEHPFHVVKNLFRHRKVRYKGLARNTAQLFSLFALANLVIAKNQLL
STHGSNPSCV

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1789921, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1789612, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1789355, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1788518, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1788342, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1787592, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1786877, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1786764, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1786453, Length=316, Percent_Identity=53.1645569620253, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1787634, Length=313, Percent_Identity=53.0351437699681, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI1788303, Length=316, Percent_Identity=52.8481012658228, Blast_Score=335, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37081; Mature: 37081

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRQIGFAEAESAGKKRVTKRQRFLAEMEKVVPWSRLLSVIGPYYPKGERGRPPIGLERM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRIYLLQQWYGLSDEGLEDALYDSIAMRAFAGIDLARENVPDATTLLKFRRLLVEHALTR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KLFDEIGIELCERGLMMKEGTLVDATIFEAAPSTKNAGKSRDPEMHQTKKGNDWYFGMKA
HHHHHHHHHHHHCCCEECCCCEEEHHHHHCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEE
HVGVDADSGLVHSVVTTAANEPDVSQAHALLHGHEQEAFGDAGYTGVDKREEMKGKTVKW
EECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHCCCEEEE
HVALKRGKIRAMQEGPLKDLVIAVERTKAQIRARVEHPFHVVKNLFRHRKVRYKGLARNT
EEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQLFSLFALANLVIAKNQLLSTHGSNPSCV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQRQIGFAEAESAGKKRVTKRQRFLAEMEKVVPWSRLLSVIGPYYPKGERGRPPIGLERM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRIYLLQQWYGLSDEGLEDALYDSIAMRAFAGIDLARENVPDATTLLKFRRLLVEHALTR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KLFDEIGIELCERGLMMKEGTLVDATIFEAAPSTKNAGKSRDPEMHQTKKGNDWYFGMKA
HHHHHHHHHHHHCCCEECCCCEEEHHHHHCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEE
HVGVDADSGLVHSVVTTAANEPDVSQAHALLHGHEQEAFGDAGYTGVDKREEMKGKTVKW
EECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHCCCEEEE
HVALKRGKIRAMQEGPLKDLVIAVERTKAQIRARVEHPFHVVKNLFRHRKVRYKGLARNT
EEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQLFSLFALANLVIAKNQLLSTHGSNPSCV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]