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The map label for this gene is catB [H]

Identifier: 189352335

GI number: 189352335

Start: 384963

End: 386096

Strand: Reverse

Name: catB [H]

Synonym: BMULJ_03554

Alternate gene names: 189352335

Gene position: 386096-384963 (Counterclockwise)

Preceding gene: 189352337

Following gene: 189352334

Centisome position: 15.61

GC content: 68.69

Gene sequence:

>1134_bases
ATGATAGCAACAGCCGCCACGATCGAAAGCGTCGAGACGCTGCTGGTCGACGTACCGACGATCCGGCCCCACAAACTCTC
GGTCGCGACGATGAACTGCCAGACGCTCGTGCTGGTCCGCATACGATGCACGGACGGTATCGAAGGCGTCGGCGAAGCGA
CGACGATCGGCGGGCTCGCGTACGGTGAGGAGAGCCCGGAAAGCATCAAGATCAACATCGATACGTATTTCGCGCCGCTG
CTGCGCGGGATGGACGCGACGCGTCCGGGCGCCGCGATGGCGCGCGCCCGCAAGCTGTTCCAGGGCAATCGCTTCGCGAA
ATGTGCGCTCGAGACGGCGCTGTTCGATGCGCAAGCACGCCGCGTCGGCGTGCCGCTGTCCGAGTTGTTCGGCGGCCGCG
TGGCCGATGCGGTCGACGTCGCATGGACGCTCGCGAGCGGCGACACGAAGCGCGACATCGCGGAGGCCGAAGCGATGCTC
GATGCGCGCCGCCACCGCGCGTTCAAGCTCAAGATCGGGTCGAACGCGGTCGACGACGACGTCGCGCATGTGATCGCGAT
CAAGCGCGCGCTCGGCGAGCGCGGCGACGTGCGCGTCGACGTGAACCAGGCATGGACCGAAACCGACGCGATCCGCGCCT
GCGCGCGGCTCGCGGATGCGGGCGTGAGCCTCGTCGAACAGCCGATCGCCGCGAGCAACCGGGCGGGCCTGCAACGGCTC
ACGCAGCTCGCGCACGTGCCCATCATGGCCGACGAGGCACTGCACGGCCCGATCGACGCGTTCGCGCTCGCACAAACACG
CGCGGCCGACGTATTCGCCGTGAAGATCGCGCAGTCCGGCGGGCTGCAAGGCGCCGCGCACGTCGCGGCGATCGCGTCGG
CGGCCGGCATCGATTTGTACGGCGGCACGATGCTCGAAGGCGCGGCCGGCACGCTCGCCTCCGCACAACTGTTCAGCACG
TTCGCTTCGCTGAAATGGGGCACCGAGCTGTTCGGCCCGCTGCTGCTGACCGAGGAAATCCTCGTCGAACCGCTGCGCTA
CGAGGACTTCAAGCTGCATCTGCCGGCGACGCCGGGCCTCGGCATCGCGTTCGACTGGGCGCGCATCGAGCGGATGCGGC
GCACCGCCCGCTGA

Upstream 100 bases:

>100_bases
CAATTCCATTTCATGCTCCGAAGGTATGGAAGTAGTCGGAAAGAGTGTTGGACGCGGCCCGGCGCGTATTCCTACCATGT
GCACCGACAGGAGGAATCGC

Downstream 100 bases:

>100_bases
TTCCATACGACAACCGAAACCGGAGACAGAGATGACCCGACAAGAAATCGACGCCCTGCTGAAGACCTTCGACGATGCCG
CGGAAAAGCCCGGCAATCCG

Product: muconate cycloisomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I 2; MLE 2; Muconate cycloisomerase I 2 [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIATAATIESVETLLVDVPTIRPHKLSVATMNCQTLVLVRIRCTDGIEGVGEATTIGGLAYGEESPESIKINIDTYFAPL
LRGMDATRPGAAMARARKLFQGNRFAKCALETALFDAQARRVGVPLSELFGGRVADAVDVAWTLASGDTKRDIAEAEAML
DARRHRAFKLKIGSNAVDDDVAHVIAIKRALGERGDVRVDVNQAWTETDAIRACARLADAGVSLVEQPIAASNRAGLQRL
TQLAHVPIMADEALHGPIDAFALAQTRAADVFAVKIAQSGGLQGAAHVAAIASAAGIDLYGGTMLEGAAGTLASAQLFST
FASLKWGTELFGPLLLTEEILVEPLRYEDFKLHLPATPGLGIAFDWARIERMRRTAR

Sequences:

>Translated_377_residues
MIATAATIESVETLLVDVPTIRPHKLSVATMNCQTLVLVRIRCTDGIEGVGEATTIGGLAYGEESPESIKINIDTYFAPL
LRGMDATRPGAAMARARKLFQGNRFAKCALETALFDAQARRVGVPLSELFGGRVADAVDVAWTLASGDTKRDIAEAEAML
DARRHRAFKLKIGSNAVDDDVAHVIAIKRALGERGDVRVDVNQAWTETDAIRACARLADAGVSLVEQPIAASNRAGLQRL
TQLAHVPIMADEALHGPIDAFALAQTRAADVFAVKIAQSGGLQGAAHVAAIASAAGIDLYGGTMLEGAAGTLASAQLFST
FASLKWGTELFGPLLLTEEILVEPLRYEDFKLHLPATPGLGIAFDWARIERMRRTAR
>Mature_377_residues
MIATAATIESVETLLVDVPTIRPHKLSVATMNCQTLVLVRIRCTDGIEGVGEATTIGGLAYGEESPESIKINIDTYFAPL
LRGMDATRPGAAMARARKLFQGNRFAKCALETALFDAQARRVGVPLSELFGGRVADAVDVAWTLASGDTKRDIAEAEAML
DARRHRAFKLKIGSNAVDDDVAHVIAIKRALGERGDVRVDVNQAWTETDAIRACARLADAGVSLVEQPIAASNRAGLQRL
TQLAHVPIMADEALHGPIDAFALAQTRAADVFAVKIAQSGGLQGAAHVAAIASAAGIDLYGGTMLEGAAGTLASAQLFST
FASLKWGTELFGPLLLTEEILVEPLRYEDFKLHLPATPGLGIAFDWARIERMRRTAR

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=312, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 40165; Mature: 40165

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATAATIESVETLLVDVPTIRPHKLSVATMNCQTLVLVRIRCTDGIEGVGEATTIGGLA
CCCCHHHHHHHHHHHEECCCCCCCEEEEEEECCEEEEEEEEEECCCCCCCCCCCEECCEE
YGEESPESIKINIDTYFAPLLRGMDATRPGAAMARARKLFQGNRFAKCALETALFDAQAR
CCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RVGVPLSELFGGRVADAVDVAWTLASGDTKRDIAEAEAMLDARRHRAFKLKIGSNAVDDD
HCCCCHHHHHCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
VAHVIAIKRALGERGDVRVDVNQAWTETDAIRACARLADAGVSLVEQPIAASNRAGLQRL
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHH
TQLAHVPIMADEALHGPIDAFALAQTRAADVFAVKIAQSGGLQGAAHVAAIASAAGIDLY
HHHHCCCCEECCHHCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEC
GGTMLEGAAGTLASAQLFSTFASLKWGTELFGPLLLTEEILVEPLRYEDFKLHLPATPGL
CCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
GIAFDWARIERMRRTAR
CEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MIATAATIESVETLLVDVPTIRPHKLSVATMNCQTLVLVRIRCTDGIEGVGEATTIGGLA
CCCCHHHHHHHHHHHEECCCCCCCEEEEEEECCEEEEEEEEEECCCCCCCCCCCEECCEE
YGEESPESIKINIDTYFAPLLRGMDATRPGAAMARARKLFQGNRFAKCALETALFDAQAR
CCCCCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RVGVPLSELFGGRVADAVDVAWTLASGDTKRDIAEAEAMLDARRHRAFKLKIGSNAVDDD
HCCCCHHHHHCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHH
VAHVIAIKRALGERGDVRVDVNQAWTETDAIRACARLADAGVSLVEQPIAASNRAGLQRL
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHH
TQLAHVPIMADEALHGPIDAFALAQTRAADVFAVKIAQSGGLQGAAHVAAIASAAGIDLY
HHHHCCCCEECCHHCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCEEC
GGTMLEGAAGTLASAQLFSTFASLKWGTELFGPLLLTEEILVEPLRYEDFKLHLPATPGL
CCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
GIAFDWARIERMRRTAR
CEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9260969 [H]