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The map label for this gene is tktB [C]

Identifier: 189352305

GI number: 189352305

Start: 353993

End: 354838

Strand: Reverse

Name: tktB [C]

Synonym: BMULJ_03524

Alternate gene names: 189352305

Gene position: 354838-353993 (Counterclockwise)

Preceding gene: 189352306

Following gene: 189352304

Centisome position: 14.35

GC content: 66.08

Gene sequence:

>846_bases
ATGGATAGCGAAGTCATGACCGAAGACGTCGCGCTCGCCGAGCGCGCGTACCGGATCAGGAGAAACGCGCTGCGCATGGG
CGAAGTGCAGGGTCAGGGCTATATCGGCCAGGCGCTCGACATCGCCGACGTGCTCGCGGTCGCATACTTCGGCGCGATGC
GCTACCGCCCGCAGGACCCGGACTGGGAAGCGCGCGACCGCTTTCTGCTGTCGAACGGGCATTACGCGATCGCGCTCTAC
GCGGCGCTGTTCGAAGCGGGCGTGCTGCCCGCCGACGAGCTCGAGACGTACGGCAGCGACGACAGCCGCCTGCCGATGTC
CGGGATGGCGAGCTACACGCCGGGGATGGAGATGTCGGGCGGCTCGCTCGGGCAGGGACTGACGATCGCCGTCGGCCGCT
GTCTCGGCCTCAAGCGCAAGGGATCGGACGCCTTCGTGTACACGCTGTTCTCCGACGGCGAGCTCGACGAGGGCGCAATC
TGGGAAGGACTGATGTCGGCCGCGCACTGGCAGCTCGACAACCTGATCGCGATCGTCGACGTGAACAATCAGCAGGCCGA
CGGCCCGTCGACGCAGATCATGGCGTTCGAGCCGCTCGTCGACAAGCTCGAAGCATTCGGCTGGTACGTGCAGCGCGTGA
ACGGCAACGACATCGACGCGGTCAAGCAGGCGTTCGACAACGCACGCCGCCACGATCGGCCGCAGCCGCGCATCATCGTC
TGCGATACGAAGATGGGGTGCGGCGTGCCGTTCCTCGAACAACGCGAGAAGAACCATTTCATCCGCGTCGACGCGCACGA
GTGGCAGCTCGCGCTCGACGCACTCGAAGCCGGGAGACAAGCATGA

Upstream 100 bases:

>100_bases
GGCCGCGGTCACGCTGGGCGCCGCGCTCGTATTCGCCGTGCCGGCCCGGTCCGTGAACCGCTGACGCGCCGGCACACTTT
CGACCTCACAGGAGCAACAG

Downstream 100 bases:

>100_bases
GTACCGCCGCCCAGAAGCCGCGCCTGAAAACCTCCGCGATGATCGCATCGATCGCGGCCGAAGGACAGCCGACGCGCGCC
GCACCGTTCGGTCACGCCCT

Product: putative transketolase

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDSEVMTEDVALAERAYRIRRNALRMGEVQGQGYIGQALDIADVLAVAYFGAMRYRPQDPDWEARDRFLLSNGHYAIALY
AALFEAGVLPADELETYGSDDSRLPMSGMASYTPGMEMSGGSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAI
WEGLMSAAHWQLDNLIAIVDVNNQQADGPSTQIMAFEPLVDKLEAFGWYVQRVNGNDIDAVKQAFDNARRHDRPQPRIIV
CDTKMGCGVPFLEQREKNHFIRVDAHEWQLALDALEAGRQA

Sequences:

>Translated_281_residues
MDSEVMTEDVALAERAYRIRRNALRMGEVQGQGYIGQALDIADVLAVAYFGAMRYRPQDPDWEARDRFLLSNGHYAIALY
AALFEAGVLPADELETYGSDDSRLPMSGMASYTPGMEMSGGSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAI
WEGLMSAAHWQLDNLIAIVDVNNQQADGPSTQIMAFEPLVDKLEAFGWYVQRVNGNDIDAVKQAFDNARRHDRPQPRIIV
CDTKMGCGVPFLEQREKNHFIRVDAHEWQLALDALEAGRQA
>Mature_281_residues
MDSEVMTEDVALAERAYRIRRNALRMGEVQGQGYIGQALDIADVLAVAYFGAMRYRPQDPDWEARDRFLLSNGHYAIALY
AALFEAGVLPADELETYGSDDSRLPMSGMASYTPGMEMSGGSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAI
WEGLMSAAHWQLDNLIAIVDVNNQQADGPSTQIMAFEPLVDKLEAFGWYVQRVNGNDIDAVKQAFDNARRHDRPQPRIIV
CDTKMGCGVPFLEQREKNHFIRVDAHEWQLALDALEAGRQA

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=252, Percent_Identity=35.3174603174603, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI205277463, Length=253, Percent_Identity=35.1778656126482, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI4507521, Length=253, Percent_Identity=35.1778656126482, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI225637459, Length=249, Percent_Identity=28.5140562248996, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI225637461, Length=218, Percent_Identity=29.8165137614679, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI225637463, Length=216, Percent_Identity=30.0925925925926, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1788808, Length=267, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI48994911, Length=251, Percent_Identity=33.8645418326693, Blast_Score=117, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17539652, Length=252, Percent_Identity=30.952380952381, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6325331, Length=244, Percent_Identity=35.2459016393443, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319593, Length=230, Percent_Identity=32.1739130434783, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24666278, Length=263, Percent_Identity=31.9391634980989, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45551847, Length=253, Percent_Identity=32.8063241106719, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45550715, Length=253, Percent_Identity=32.8063241106719, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645119, Length=220, Percent_Identity=35, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 31141; Mature: 31141

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSEVMTEDVALAERAYRIRRNALRMGEVQGQGYIGQALDIADVLAVAYFGAMRYRPQDP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DWEARDRFLLSNGHYAIALYAALFEAGVLPADELETYGSDDSRLPMSGMASYTPGMEMSG
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
GSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAIWEGLMSAAHWQLDNLIAIVD
CCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
VNNQQADGPSTQIMAFEPLVDKLEAFGWYVQRVNGNDIDAVKQAFDNARRHDRPQPRIIV
CCCCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEE
CDTKMGCGVPFLEQREKNHFIRVDAHEWQLALDALEAGRQA
EECCCCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDSEVMTEDVALAERAYRIRRNALRMGEVQGQGYIGQALDIADVLAVAYFGAMRYRPQDP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
DWEARDRFLLSNGHYAIALYAALFEAGVLPADELETYGSDDSRLPMSGMASYTPGMEMSG
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
GSLGQGLTIAVGRCLGLKRKGSDAFVYTLFSDGELDEGAIWEGLMSAAHWQLDNLIAIVD
CCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
VNNQQADGPSTQIMAFEPLVDKLEAFGWYVQRVNGNDIDAVKQAFDNARRHDRPQPRIIV
CCCCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEE
CDTKMGCGVPFLEQREKNHFIRVDAHEWQLALDALEAGRQA
EECCCCCCCCHHHHHCCCCEEEEECHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]