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The map label for this gene is argG [H]

Identifier: 189352280

GI number: 189352280

Start: 327468

End: 328805

Strand: Reverse

Name: argG [H]

Synonym: BMULJ_03499

Alternate gene names: 189352280

Gene position: 328805-327468 (Counterclockwise)

Preceding gene: 189352281

Following gene: 189352277

Centisome position: 13.29

GC content: 64.2

Gene sequence:

>1338_bases
ATGAGCACGATTCTCGAAAGCCTCCCGACCGGCCAGAAGGTCGGTATCGCCTTCTCCGGCGGCCTGGACACCAGCGCCGC
GCTGCACTGGATGAAACTGAAGGGCGCCGTCCCGTACGCGTACACGGCAAACCTCGGCCAGCCCGACGAAGACGATTACG
ATGCGATCCCGAAACGTGCGCTCGAATACGGTGCGGCCGGTGCCCGCCTGATCGACTGCCGCGCGCAACTCGTCGCCGAA
GGCATCGCCGCGCTGCAAAGCGGCGCGTTCCACATCACGACCGCCGGCGTCACGTACTTCAACACGACGCCGATCGGCCG
CGCCGTGACGGGCACGATGCTCGTCGCCGCGATGAAGGAAGACGGCGTGAACATCTGGGGCGACGGCAGCACGTACAAGG
GCAACGACATCGAGCGCTTCTACCGCTACGGTCTGCTCGTGAACCCGGATCTGAAGATCTACAAGCCGTGGCTCGACCAG
ACGTTCATCGACGAACTCGGCGGCCGCGCGGAAATGTCCGAATTCATGCGTCAGTCGGGCTTCGCGTACAAGATGTCGGC
CGAGAAGGCGTATTCGACCGACTCGAACCTGCTCGGCGCGACGCACGAGGCGAAGGATCTGGAAAGCCTCGAAAGCGGGA
TCAAGATCGTGAACCCGATCATGGGCGTCGCGTTCTGGCGCGACGACGTGAAGATCGCCGCCGAAGAAGTGACGGTGCGC
TTCGAAGCCGGCCAGCCGGTCGCGCTGAACGGCGTCGAGTTCAAGGATCAGGTCGAGTTGCTGCTCGAGGCGAACCGCAT
CGGCGGCCGTCACGGCCTCGGGATGAGCGACCAGATCGAGAACCGCATCATCGAGGCGAAGAGCCGCGGCATCTATGAAG
CGCCGGGGCTCGCGCTGCTGTATATCGCTTACGAGCGTCTCGTGACGGGCATCCACAACGAAGACACGATCGAGCAGTAC
CGCGAGAACGGTCGCCGTCTCGGTCGCCTGCTGTATCAGGGCCGCTGGTTCGACCCGCAGGCGATCATGCTGCGCGAAAC
GGCCCAGCGCTGGGTCGCGCGCGCGATCACCGGCGAAGTGAAGATCGAGCTGCGCCGCGGCAACGACTATTCGATTCTCA
GCACGAAGTCGCCGAACCTCACGTATCAGCCGGAGCGTCTGTCGATGGAGAAGGTCGCGTCGACGTTCTCGCCGCGCGAC
CGGATCGGTCAGCTGACGATGCGCAATCTCGACATCACCGATACGCGCGACAAGCTGCGCGTGTACACGCAGGTCGGGCT
GCTGACGCCGGGTGAAGCGTCGGCGCTGCCGCAAATCAAGGGCGATAGCGGGGAGTAA

Upstream 100 bases:

>100_bases
ATTTCCGCGAAATCAGCCCTTGCCGCAACTTCCCGGACAGCCGATACTCGCGTATTCCGCGAAATACGATCCCACCGGAC
TTCAATCCACGGTAATTCCT

Downstream 100 bases:

>100_bases
ACCGCTTGCCCGCGGCCGGGTGGCGGGAATGCCGCCCGGCCGGTTGCCTGTCGAATCTGCGTTTCCCCTCGCCTTCCCCT
CTCCCTTCCGCTCCCTCCCG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSTILESLPTGQKVGIAFSGGLDTSAALHWMKLKGAVPYAYTANLGQPDEDDYDAIPKRALEYGAAGARLIDCRAQLVAE
GIAALQSGAFHITTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLVNPDLKIYKPWLDQ
TFIDELGGRAEMSEFMRQSGFAYKMSAEKAYSTDSNLLGATHEAKDLESLESGIKIVNPIMGVAFWRDDVKIAAEEVTVR
FEAGQPVALNGVEFKDQVELLLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLYIAYERLVTGIHNEDTIEQY
RENGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAITGEVKIELRRGNDYSILSTKSPNLTYQPERLSMEKVASTFSPRD
RIGQLTMRNLDITDTRDKLRVYTQVGLLTPGEASALPQIKGDSGE

Sequences:

>Translated_445_residues
MSTILESLPTGQKVGIAFSGGLDTSAALHWMKLKGAVPYAYTANLGQPDEDDYDAIPKRALEYGAAGARLIDCRAQLVAE
GIAALQSGAFHITTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLVNPDLKIYKPWLDQ
TFIDELGGRAEMSEFMRQSGFAYKMSAEKAYSTDSNLLGATHEAKDLESLESGIKIVNPIMGVAFWRDDVKIAAEEVTVR
FEAGQPVALNGVEFKDQVELLLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLYIAYERLVTGIHNEDTIEQY
RENGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAITGEVKIELRRGNDYSILSTKSPNLTYQPERLSMEKVASTFSPRD
RIGQLTMRNLDITDTRDKLRVYTQVGLLTPGEASALPQIKGDSGE
>Mature_444_residues
STILESLPTGQKVGIAFSGGLDTSAALHWMKLKGAVPYAYTANLGQPDEDDYDAIPKRALEYGAAGARLIDCRAQLVAEG
IAALQSGAFHITTAGVTYFNTTPIGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLVNPDLKIYKPWLDQT
FIDELGGRAEMSEFMRQSGFAYKMSAEKAYSTDSNLLGATHEAKDLESLESGIKIVNPIMGVAFWRDDVKIAAEEVTVRF
EAGQPVALNGVEFKDQVELLLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLYIAYERLVTGIHNEDTIEQYR
ENGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAITGEVKIELRRGNDYSILSTKSPNLTYQPERLSMEKVASTFSPRDR
IGQLTMRNLDITDTRDKLRVYTQVGLLTPGEASALPQIKGDSGE

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=408, Percent_Identity=29.4117647058824, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI16950633, Length=408, Percent_Identity=29.4117647058824, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1789563, Length=440, Percent_Identity=79.5454545454545, Blast_Score=734, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=419, Percent_Identity=30.3102625298329, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358151, Length=424, Percent_Identity=28.5377358490566, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 49253; Mature: 49121

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILESLPTGQKVGIAFSGGLDTSAALHWMKLKGAVPYAYTANLGQPDEDDYDAIPKRA
CCCHHHHCCCCCEEEEEEECCCCHHHHEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHH
LEYGAAGARLIDCRAQLVAEGIAALQSGAFHITTAGVTYFNTTPIGRAVTGTMLVAAMKE
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHHHHEEHHC
DGVNIWGDGSTYKGNDIERFYRYGLLVNPDLKIYKPWLDQTFIDELGGRAEMSEFMRQSG
CCCEEECCCCCCCCCHHHHHHHCCCEECCCCEEECCHHHHHHHHHHCCHHHHHHHHHHCC
FAYKMSAEKAYSTDSNLLGATHEAKDLESLESGIKIVNPIMGVAFWRDDVKIAAEEVTVR
CEEEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEEE
FEAGQPVALNGVEFKDQVELLLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
EECCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
YIAYERLVTGIHNEDTIEQYRENGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAITGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEE
KIELRRGNDYSILSTKSPNLTYQPERLSMEKVASTFSPRDRIGQLTMRNLDITDTRDKLR
EEEEEECCCEEEEEECCCCCEECHHHHHHHHHHHHCCCHHHHHHHEEECCCCCCCHHHHE
VYTQVGLLTPGEASALPQIKGDSGE
EEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STILESLPTGQKVGIAFSGGLDTSAALHWMKLKGAVPYAYTANLGQPDEDDYDAIPKRA
CCHHHHCCCCCEEEEEEECCCCHHHHEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHH
LEYGAAGARLIDCRAQLVAEGIAALQSGAFHITTAGVTYFNTTPIGRAVTGTMLVAAMKE
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHHHHEEHHC
DGVNIWGDGSTYKGNDIERFYRYGLLVNPDLKIYKPWLDQTFIDELGGRAEMSEFMRQSG
CCCEEECCCCCCCCCHHHHHHHCCCEECCCCEEECCHHHHHHHHHHCCHHHHHHHHHHCC
FAYKMSAEKAYSTDSNLLGATHEAKDLESLESGIKIVNPIMGVAFWRDDVKIAAEEVTVR
CEEEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEEE
FEAGQPVALNGVEFKDQVELLLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
EECCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
YIAYERLVTGIHNEDTIEQYRENGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAITGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEE
KIELRRGNDYSILSTKSPNLTYQPERLSMEKVASTFSPRDRIGQLTMRNLDITDTRDKLR
EEEEEECCCEEEEEECCCCCEECHHHHHHHHHHHHCCCHHHHHHHEEECCCCCCCHHHHE
VYTQVGLLTPGEASALPQIKGDSGE
EEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA