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The map label for this gene is potB [H]

Identifier: 189352239

GI number: 189352239

Start: 279916

End: 280893

Strand: Reverse

Name: potB [H]

Synonym: BMULJ_03457

Alternate gene names: 189352239

Gene position: 280893-279916 (Counterclockwise)

Preceding gene: 189352240

Following gene: 189352238

Centisome position: 11.36

GC content: 69.73

Gene sequence:

>978_bases
ATGGGACAGCCTACCCGCCCGCTGCAGGCCGACGGCGCACCGGCCGGCGTCGCGCCGCGCAGCGCGGCCGAGCCCGTACC
GGCCCGCGTGGCCGCCGGCCGCGCATGGCGCGCGCGCTGGCGTGCGCATGCGCCGCTGTGGCTGATGTGCGCGCCGGCGT
TCGCGCTGTTCGCCGCGCTCGTGCTCGTGCCGCTCGCGATGACGTTCGCGTTGACGTTCTACCGCTTCGACCCGGCCACC
GGCCCGATCGCCGCGTTCCAGTTCGGCAACTACGCGGAAGTGCTCGGCTCGTCCTACTTCCATACGATCTTCGCGCGCAC
GTTCGGCATCGCCGCGCTGACCACCGCGATCTGCGTCGCGATCGGCACGCCCGAAGCCTACGTGCTGTCGAAAATGCGCG
ACCCGTGGCGCTCGATGTTCCTGCTCGCGATCCTCGCGCCGCTGCTCGTGTCGGTCGTCGTGCGCGCATTCGGCTGGAGC
ATGCTGCTGAACACAGGCGGACTCGTGAACCGCACGCTCGCGCTCGTCGGCCTCGGCCCGTACAAGCTCGAATACACGAC
GTTCGCGATCGTGATCGCACTCGTGCATGTGATGCTGCCGTTCATGGTGATTCCGGTCTGGACGGCGCTGCAGCGGCTCG
ATCCGCAAACCGAATACGCGGCGCTGTCGCTCGGCGCGTCGCCGTTTACGACGCTGCGCCGCATCGTCGTGCCGCAGCTG
ATGCCCGGCGTGCTGTCGGGCAGCCTGATGGTGTTCGGGCTGTCGGCGAGCGCGTTCGCGATTCCCGGCCTGCTCGGCGG
ACGCCGGCTCAAGGTCGCGGCAACGGCCGTCTACGACGAATTCCTCGGCTCGCTGAACTGGCCGCTCGGCGCGACGATCG
CGGTGCTGCTGCTCGTCGCGAACCTCGTCGTGATGCTCACCTACTACCGCGTGCTCGAACGCCGCTACGCGCGCAGCCTC
GGAGGCGCTTCCCGATGA

Upstream 100 bases:

>100_bases
GTGCTGCCAGAACGAAGGCGCGGAGCCGCTCGCCGAAGGCGCGCCGGTCGGCATCGACTGGCACAGCGACGCGGTGCGCG
TGATGCGCCGGGAGGCCTGA

Downstream 100 bases:

>100_bases
GAAAGAACGGCCCGTTCGCGCTTGCGTTCCATACGCTCGTGATCCTGTTCGTGCTCGCGCCGCTCGCGATCGTCGTGCTG
GTCGCCTTCACGCCCGACGA

Product: spermidine/putrescine transport system permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MGQPTRPLQADGAPAGVAPRSAAEPVPARVAAGRAWRARWRAHAPLWLMCAPAFALFAALVLVPLAMTFALTFYRFDPAT
GPIAAFQFGNYAEVLGSSYFHTIFARTFGIAALTTAICVAIGTPEAYVLSKMRDPWRSMFLLAILAPLLVSVVVRAFGWS
MLLNTGGLVNRTLALVGLGPYKLEYTTFAIVIALVHVMLPFMVIPVWTALQRLDPQTEYAALSLGASPFTTLRRIVVPQL
MPGVLSGSLMVFGLSASAFAIPGLLGGRRLKVAATAVYDEFLGSLNWPLGATIAVLLLVANLVVMLTYYRVLERRYARSL
GGASR

Sequences:

>Translated_325_residues
MGQPTRPLQADGAPAGVAPRSAAEPVPARVAAGRAWRARWRAHAPLWLMCAPAFALFAALVLVPLAMTFALTFYRFDPAT
GPIAAFQFGNYAEVLGSSYFHTIFARTFGIAALTTAICVAIGTPEAYVLSKMRDPWRSMFLLAILAPLLVSVVVRAFGWS
MLLNTGGLVNRTLALVGLGPYKLEYTTFAIVIALVHVMLPFMVIPVWTALQRLDPQTEYAALSLGASPFTTLRRIVVPQL
MPGVLSGSLMVFGLSASAFAIPGLLGGRRLKVAATAVYDEFLGSLNWPLGATIAVLLLVANLVVMLTYYRVLERRYARSL
GGASR
>Mature_324_residues
GQPTRPLQADGAPAGVAPRSAAEPVPARVAAGRAWRARWRAHAPLWLMCAPAFALFAALVLVPLAMTFALTFYRFDPATG
PIAAFQFGNYAEVLGSSYFHTIFARTFGIAALTTAICVAIGTPEAYVLSKMRDPWRSMFLLAILAPLLVSVVVRAFGWSM
LLNTGGLVNRTLALVGLGPYKLEYTTFAIVIALVHVMLPFMVIPVWTALQRLDPQTEYAALSLGASPFTTLRRIVVPQLM
PGVLSGSLMVFGLSASAFAIPGLLGGRRLKVAATAVYDEFLGSLNWPLGATIAVLLLVANLVVMLTYYRVLERRYARSLG
GASR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=252, Percent_Identity=29.7619047619048, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=28.5714285714286, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787713, Length=209, Percent_Identity=28.2296650717703, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34973; Mature: 34842

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQPTRPLQADGAPAGVAPRSAAEPVPARVAAGRAWRARWRAHAPLWLMCAPAFALFAAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
VLVPLAMTFALTFYRFDPATGPIAAFQFGNYAEVLGSSYFHTIFARTFGIAALTTAICVA
HHHHHHHHHHHHHHEECCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IGTPEAYVLSKMRDPWRSMFLLAILAPLLVSVVVRAFGWSMLLNTGGLVNRTLALVGLGP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
YKLEYTTFAIVIALVHVMLPFMVIPVWTALQRLDPQTEYAALSLGASPFTTLRRIVVPQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHH
MPGVLSGSLMVFGLSASAFAIPGLLGGRRLKVAATAVYDEFLGSLNWPLGATIAVLLLVA
HHHHHCCCHHEEECCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
NLVVMLTYYRVLERRYARSLGGASR
HHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GQPTRPLQADGAPAGVAPRSAAEPVPARVAAGRAWRARWRAHAPLWLMCAPAFALFAAL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]