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The map label for this gene is rpfF [C]

Identifier: 189352177

GI number: 189352177

Start: 214504

End: 215367

Strand: Reverse

Name: rpfF [C]

Synonym: BMULJ_03395

Alternate gene names: 189352177

Gene position: 215367-214504 (Counterclockwise)

Preceding gene: 189352179

Following gene: 189352172

Centisome position: 8.71

GC content: 64.35

Gene sequence:

>864_bases
ATGCAGCTCCAATCACATCCCGCCTGCCGCCCGTTCTATGAAGCCGGCGAACTCACGCAGCTCACCGCTTTTTATGAGGA
AGGCAGAAACATCATGTGGATGATGCTCCGATCCGAACCGCGGCCGTGCTTCAACCAGCAACTCGTCACCGACATCATCC
ATCTCGCGCGCGTCGCCCGCGACTCGGGCCTGCGATTCGACTTCTGGGTCACCGGCTCGCTCGTGCCCGAGCTGTTCAAT
GTCGGCGGCGACCTGAGCTTCTTCGTCGACGCGATCCGCAGCGGCCGCCGCGATCTGCTGATGGCGTACGCGCGCTCGTG
CATCGACGGCGTGTACGAGATCTACACCGGCTTCGGCACGGGCGCGATCTCGATCGCGATGGTCGAAGGCAGCGCGCTCG
GCGGCGGTTTCGAGGCCGCGCTCGCGCATCACTACGTGCTCGCGCAGAAGGGCGTGAAGCTCGGCTTCCCGGAAATCGCG
TTCAACCTGTTTCCGGGGATGGGCGGCTATTCGCTCGTCGCGCGCAAGGCCGACCGCAGTCTCGCCGAAACGCTGATTGC
GACCGGCGAAGCGCACGCCGCCGAGTGGTACGAGGATCGCGGGCTCGTCGATCAAACGTTCGAACCCGGCGACGCGTATC
TCGCGACGCGCACCTTCATCGACGTGATGAAGCCGAAGCTGAACGGGATCCGCGCGATGCTGCGCGCACGCGAGCGCGTG
TTCCAGCTCACGCGTTCCGAGCTGATGGACATCACCGAGGACTGGGTGCATTCGGCGTTCACGATCGAGCCGAAGGATCT
CGCCTACATGGAACGCCTCGTGATGCTGCAGAACCGCCGCGTGTCGAAGTTGCGCACGGTGTGA

Upstream 100 bases:

>100_bases
ACAATGGCGATGCCTCGCGTGCGGCGCCCGGCGCGGCCGCTCCGCCACGCACGGCGCCGCACGCGTGGTCTTTCAAAAAC
CACATTCAACGAGGATTGCC

Downstream 100 bases:

>100_bases
TGCAACGGCTGTCGCCTTGCGGCGACAGCCGGTTCGGGAACGCTCAGGCGATCAGCCTGAGCTTTCTCGTCTCCGCGAGC
CACGCCTCGAACGCGTGGGC

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: Enoyl-CoA Hydratase/Isomerase; Enoyl-Coa Hydratase Protein; Enoyl-CoA Hydratase/Isomerase Family; Enoyl-CoA Hydratase/Isomerase Family Protein

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MQLQSHPACRPFYEAGELTQLTAFYEEGRNIMWMMLRSEPRPCFNQQLVTDIIHLARVARDSGLRFDFWVTGSLVPELFN
VGGDLSFFVDAIRSGRRDLLMAYARSCIDGVYEIYTGFGTGAISIAMVEGSALGGGFEAALAHHYVLAQKGVKLGFPEIA
FNLFPGMGGYSLVARKADRSLAETLIATGEAHAAEWYEDRGLVDQTFEPGDAYLATRTFIDVMKPKLNGIRAMLRARERV
FQLTRSELMDITEDWVHSAFTIEPKDLAYMERLVMLQNRRVSKLRTV

Sequences:

>Translated_287_residues
MQLQSHPACRPFYEAGELTQLTAFYEEGRNIMWMMLRSEPRPCFNQQLVTDIIHLARVARDSGLRFDFWVTGSLVPELFN
VGGDLSFFVDAIRSGRRDLLMAYARSCIDGVYEIYTGFGTGAISIAMVEGSALGGGFEAALAHHYVLAQKGVKLGFPEIA
FNLFPGMGGYSLVARKADRSLAETLIATGEAHAAEWYEDRGLVDQTFEPGDAYLATRTFIDVMKPKLNGIRAMLRARERV
FQLTRSELMDITEDWVHSAFTIEPKDLAYMERLVMLQNRRVSKLRTV
>Mature_287_residues
MQLQSHPACRPFYEAGELTQLTAFYEEGRNIMWMMLRSEPRPCFNQQLVTDIIHLARVARDSGLRFDFWVTGSLVPELFN
VGGDLSFFVDAIRSGRRDLLMAYARSCIDGVYEIYTGFGTGAISIAMVEGSALGGGFEAALAHHYVLAQKGVKLGFPEIA
FNLFPGMGGYSLVARKADRSLAETLIATGEAHAAEWYEDRGLVDQTFEPGDAYLATRTFIDVMKPKLNGIRAMLRARERV
FQLTRSELMDITEDWVHSAFTIEPKDLAYMERLVMLQNRRVSKLRTV

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787659, Length=90, Percent_Identity=38.8888888888889, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 32297; Mature: 32297

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLQSHPACRPFYEAGELTQLTAFYEEGRNIMWMMLRSEPRPCFNQQLVTDIIHLARVAR
CCCCCCCCCCCCHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
DSGLRFDFWVTGSLVPELFNVGGDLSFFVDAIRSGRRDLLMAYARSCIDGVYEIYTGFGT
HCCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
GAISIAMVEGSALGGGFEAALAHHYVLAQKGVKLGFPEIAFNLFPGMGGYSLVARKADRS
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
LAETLIATGEAHAAEWYEDRGLVDQTFEPGDAYLATRTFIDVMKPKLNGIRAMLRARERV
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQLTRSELMDITEDWVHSAFTIEPKDLAYMERLVMLQNRRVSKLRTV
HHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MQLQSHPACRPFYEAGELTQLTAFYEEGRNIMWMMLRSEPRPCFNQQLVTDIIHLARVAR
CCCCCCCCCCCCHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
DSGLRFDFWVTGSLVPELFNVGGDLSFFVDAIRSGRRDLLMAYARSCIDGVYEIYTGFGT
HCCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
GAISIAMVEGSALGGGFEAALAHHYVLAQKGVKLGFPEIAFNLFPGMGGYSLVARKADRS
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
LAETLIATGEAHAAEWYEDRGLVDQTFEPGDAYLATRTFIDVMKPKLNGIRAMLRARERV
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQLTRSELMDITEDWVHSAFTIEPKDLAYMERLVMLQNRRVSKLRTV
HHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA