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The map label for this gene is poxB [H]

Identifier: 189352096

GI number: 189352096

Start: 117108

End: 118814

Strand: Direct

Name: poxB [H]

Synonym: BMULJ_03314

Alternate gene names: 189352096

Gene position: 117108-118814 (Clockwise)

Preceding gene: 189352095

Following gene: 189352097

Centisome position: 4.74

GC content: 66.37

Gene sequence:

>1707_bases
ATGGCGGAATATCTGGCGAAGACGCTGGCGGCAGCGGGCGTCGAGCGTATCTGGGGCGTGACCGGCGACAGCCTGAACGG
CCTGTCGTTCAGCCTGAGCCAGATCGGCTCGATCCGGTGGATGCATACGCGTCACGAGGAAAGCGCGGCGTTCGCGGCCG
GCGCCGATGCCGCGTCGACCGGGCGGCTCGCTGTATGCGCGGGCAGTTGCGGCCCCGGCAACCTGCACCTGATCAACGGG
CTCTACGACTGCCATCGCAATCATCAGCCGGTGCTCGCGATTGCCGCGCACATCCCGTCGACCGAGATCGGCCTCGGCTA
CTTTCAGGAAACCCATCCACAGGAACTGTTCCGCGAATGCAGCCACTTTGCGGAACTGGTAACCAACGCGTCGCAGTTCC
CGCGCCTGCTTGCGCGCGCGATGCGCACGGCAATCGAAGAGCGCGGCGTGGCCGTGATCGTGCTGCCGGGCGACATCGCG
CTCGGCGACGGTCCCGACGAAGCGCCGCGCTGGCACGACGCGGCACCGCCGTCGATCGTGCCGGCCGACGCCGATCTCGA
TCGACTGGCGGCTTTGCTGAACGCGTCCGACGCGGTCACGCTGCTCTGCGGCAGCGGCACGCAAGGCGCGCACGACGAAG
TCGTCGCGCTGGCCGATACGCTCGGCGCGCCGGTCGTGCATGCGTTGCGCGGCAAGCAGTTCGTCGAATGGGACAACCCG
TTCGACGTCGGGATGACGGGGCTGATCGGTTTCAGTTCCGGCTATCACGCGATGGAGTCGTGCGACACGCTGCTGATGCT
CGGCACGGATTTTCCGTACCGGCCGTTCTATCCGTCGAATGCGAAGATCGCGCAGATCGACTGGAAGGGGTCGCAGCTCG
GCCATCGCGCGCCGCTCGCGCTCGGGTTGGTCGGCACCGTGAAGGAAACGATCGCGGCGCTGCTGCCGCGCCTGACACGC
AAGACGCAGCGGCGCTTCCTCGAGAACGCGCTGAAGCACTATGCGGCCGCGCGCAAGGGGCTCGACGATCTCGCGGTGGC
CGAGCCGCCCGGCCGCGCGATTCATCCGCAATACCTGACGAAGATCGTCGACGAAGTCGCGGCCGACGATGCGATCTTCA
CGGCCGATGTCGGCACGCCGACGCTATGGGCCGCGCGCTATCTGACGATGAACGGCAAGCGTCAGCTGCACGGCTCGTTC
AATCACGGCTCGATGGCGAACGCGATGCCGCAGGCGCTCGGCGCGCAGGGCGCGCATCCGGGGCGGCAGGTCGTGTCGCT
GTCGGGCGACGGCGGGCTGTCGATGCTGCTTGGCGATCTGCTGAGCGCGCGGCAGCTCAATCTGCCGATCAAGATCGTCG
TCTATAACAACAGCCTGCTCGGCTTCGTGTCGATGGAGCTGAAGGCGGCCGGGTATCTCGATACGAACGTCGATCTGAGT
GCGACGGATTTTGCGGCGATCGCGAAGGGCGCGGGCATTTTCAGCGTACGCGTCGAACATTCAGAGAACGTCGAGCACGC
GCTGCGCACGGCGTTCGCGCACGACGGGCCGGCGCTCGTCGACGTCGTCACGTCGAAGTACGAGCTGGCCATGCCGCCGA
AGGTCGAACTCGCGCATGCGAAGGGATTCAGCTTGTTCATGCTGCGGGCGATTCTGAGCGGGCGCGGGGATGAGATCGTC
GAGTTGGCGAAGACCAATTTGCGGTAA

Upstream 100 bases:

>100_bases
AGAGGTGCCGCGCCGGCACGCAGGACGCGCCGGCGCGCGCGGAAACCGGCATGTCGCGACAACCCAACCTGCGAGCGAGG
GCATCATGGCAAGACAGACG

Downstream 100 bases:

>100_bases
CGCGCCCGCGCGCGGCCGAGATACGGGCGCCACGCGCCGTTCGCCCGGCGACCCGCGCGCACCGCATCGGGCCCACCCGC
TGCAAGGCCGTGGCGGGGCC

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MAEYLAKTLAAAGVERIWGVTGDSLNGLSFSLSQIGSIRWMHTRHEESAAFAAGADAASTGRLAVCAGSCGPGNLHLING
LYDCHRNHQPVLAIAAHIPSTEIGLGYFQETHPQELFRECSHFAELVTNASQFPRLLARAMRTAIEERGVAVIVLPGDIA
LGDGPDEAPRWHDAAPPSIVPADADLDRLAALLNASDAVTLLCGSGTQGAHDEVVALADTLGAPVVHALRGKQFVEWDNP
FDVGMTGLIGFSSGYHAMESCDTLLMLGTDFPYRPFYPSNAKIAQIDWKGSQLGHRAPLALGLVGTVKETIAALLPRLTR
KTQRRFLENALKHYAAARKGLDDLAVAEPPGRAIHPQYLTKIVDEVAADDAIFTADVGTPTLWAARYLTMNGKRQLHGSF
NHGSMANAMPQALGAQGAHPGRQVVSLSGDGGLSMLLGDLLSARQLNLPIKIVVYNNSLLGFVSMELKAAGYLDTNVDLS
ATDFAAIAKGAGIFSVRVEHSENVEHALRTAFAHDGPALVDVVTSKYELAMPPKVELAHAKGFSLFMLRAILSGRGDEIV
ELAKTNLR

Sequences:

>Translated_568_residues
MAEYLAKTLAAAGVERIWGVTGDSLNGLSFSLSQIGSIRWMHTRHEESAAFAAGADAASTGRLAVCAGSCGPGNLHLING
LYDCHRNHQPVLAIAAHIPSTEIGLGYFQETHPQELFRECSHFAELVTNASQFPRLLARAMRTAIEERGVAVIVLPGDIA
LGDGPDEAPRWHDAAPPSIVPADADLDRLAALLNASDAVTLLCGSGTQGAHDEVVALADTLGAPVVHALRGKQFVEWDNP
FDVGMTGLIGFSSGYHAMESCDTLLMLGTDFPYRPFYPSNAKIAQIDWKGSQLGHRAPLALGLVGTVKETIAALLPRLTR
KTQRRFLENALKHYAAARKGLDDLAVAEPPGRAIHPQYLTKIVDEVAADDAIFTADVGTPTLWAARYLTMNGKRQLHGSF
NHGSMANAMPQALGAQGAHPGRQVVSLSGDGGLSMLLGDLLSARQLNLPIKIVVYNNSLLGFVSMELKAAGYLDTNVDLS
ATDFAAIAKGAGIFSVRVEHSENVEHALRTAFAHDGPALVDVVTSKYELAMPPKVELAHAKGFSLFMLRAILSGRGDEIV
ELAKTNLR
>Mature_567_residues
AEYLAKTLAAAGVERIWGVTGDSLNGLSFSLSQIGSIRWMHTRHEESAAFAAGADAASTGRLAVCAGSCGPGNLHLINGL
YDCHRNHQPVLAIAAHIPSTEIGLGYFQETHPQELFRECSHFAELVTNASQFPRLLARAMRTAIEERGVAVIVLPGDIAL
GDGPDEAPRWHDAAPPSIVPADADLDRLAALLNASDAVTLLCGSGTQGAHDEVVALADTLGAPVVHALRGKQFVEWDNPF
DVGMTGLIGFSSGYHAMESCDTLLMLGTDFPYRPFYPSNAKIAQIDWKGSQLGHRAPLALGLVGTVKETIAALLPRLTRK
TQRRFLENALKHYAAARKGLDDLAVAEPPGRAIHPQYLTKIVDEVAADDAIFTADVGTPTLWAARYLTMNGKRQLHGSFN
HGSMANAMPQALGAQGAHPGRQVVSLSGDGGLSMLLGDLLSARQLNLPIKIVVYNNSLLGFVSMELKAAGYLDTNVDLSA
TDFAAIAKGAGIFSVRVEHSENVEHALRTAFAHDGPALVDVVTSKYELAMPPKVELAHAKGFSLFMLRAILSGRGDEIVE
LAKTNLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=22.6785714285714, Blast_Score=109, Evalue=9e-24,
Organism=Escherichia coli, GI1787096, Length=567, Percent_Identity=63.8447971781305, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=26.4598540145985, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=24.7311827956989, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786717, Length=553, Percent_Identity=24.0506329113924, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788716, Length=540, Percent_Identity=20.9259259259259, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17531299, Length=564, Percent_Identity=25.886524822695, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=25.8407079646018, Blast_Score=105, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=604, Percent_Identity=21.3576158940397, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320816, Length=486, Percent_Identity=22.6337448559671, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19922626, Length=478, Percent_Identity=24.8953974895398, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 60573; Mature: 60442

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEYLAKTLAAAGVERIWGVTGDSLNGLSFSLSQIGSIRWMHTRHEESAAFAAGADAAST
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHCCEEEEECCCCCCHHHCCCCCCCCC
GRLAVCAGSCGPGNLHLINGLYDCHRNHQPVLAIAAHIPSTEIGLGYFQETHPQELFREC
CCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHH
SHFAELVTNASQFPRLLARAMRTAIEERGVAVIVLPGDIALGDGPDEAPRWHDAAPPSIV
HHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCC
PADADLDRLAALLNASDAVTLLCGSGTQGAHDEVVALADTLGAPVVHALRGKQFVEWDNP
CCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHCCCEEECCCCC
FDVGMTGLIGFSSGYHAMESCDTLLMLGTDFPYRPFYPSNAKIAQIDWKGSQLGHRAPLA
CCCCCHHEEECCCCCHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
LGLVGTVKETIAALLPRLTRKTQRRFLENALKHYAAARKGLDDLAVAEPPGRAIHPQYLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHH
KIVDEVAADDAIFTADVGTPTLWAARYLTMNGKRQLHGSFNHGSMANAMPQALGAQGAHP
HHHHHHHCCCCEEEECCCCCHHHHHHHEEECCCEEECCCCCCCCHHHHHHHHHCCCCCCC
GRQVVSLSGDGGLSMLLGDLLSARQLNLPIKIVVYNNSLLGFVSMELKAAGYLDTNVDLS
CCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCC
ATDFAAIAKGAGIFSVRVEHSENVEHALRTAFAHDGPALVDVVTSKYELAMPPKVELAHA
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCEEECCCCCEEEECC
KGFSLFMLRAILSGRGDEIVELAKTNLR
CCHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AEYLAKTLAAAGVERIWGVTGDSLNGLSFSLSQIGSIRWMHTRHEESAAFAAGADAAST
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHCCEEEEECCCCCCHHHCCCCCCCCC
GRLAVCAGSCGPGNLHLINGLYDCHRNHQPVLAIAAHIPSTEIGLGYFQETHPQELFREC
CCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHH
SHFAELVTNASQFPRLLARAMRTAIEERGVAVIVLPGDIALGDGPDEAPRWHDAAPPSIV
HHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCC
PADADLDRLAALLNASDAVTLLCGSGTQGAHDEVVALADTLGAPVVHALRGKQFVEWDNP
CCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHCCCEEECCCCC
FDVGMTGLIGFSSGYHAMESCDTLLMLGTDFPYRPFYPSNAKIAQIDWKGSQLGHRAPLA
CCCCCHHEEECCCCCHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
LGLVGTVKETIAALLPRLTRKTQRRFLENALKHYAAARKGLDDLAVAEPPGRAIHPQYLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHH
KIVDEVAADDAIFTADVGTPTLWAARYLTMNGKRQLHGSFNHGSMANAMPQALGAQGAHP
HHHHHHHCCCCEEEECCCCCHHHHHHHEEECCCEEECCCCCCCCHHHHHHHHHCCCCCCC
GRQVVSLSGDGGLSMLLGDLLSARQLNLPIKIVVYNNSLLGFVSMELKAAGYLDTNVDLS
CCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCC
ATDFAAIAKGAGIFSVRVEHSENVEHALRTAFAHDGPALVDVVTSKYELAMPPKVELAHA
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCEEECCCCCEEEECC
KGFSLFMLRAILSGRGDEIVELAKTNLR
CCHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]