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The map label for this gene is dmlR [H]

Identifier: 189351988

GI number: 189351988

Start: 9578

End: 10501

Strand: Direct

Name: dmlR [H]

Synonym: BMULJ_03205

Alternate gene names: 189351988

Gene position: 9578-10501 (Clockwise)

Preceding gene: 189351986

Following gene: 189351994

Centisome position: 0.39

GC content: 67.97

Gene sequence:

>924_bases
GTGAACAAATCGCCGAACCTGGAAGACCTGCGCGTGTTCAGCCTCGTCGTCCGACTGGCCAGCTTCAGCGCGGCCGCCGA
GCAGCTCGCGGTGTCGCCTGCGTACGTGAGCAAGCGCATCGCGATGCTCGAGACGCAGCTCGGCACGCGCCTGCTGCATC
GCTCGACGCGTCGCGTGACGGTGACCGAAGCGGGCGAGCGCGTGTTCGCGCATGCCGAGAAGATCCTTGACGACGTCGAT
CATCTCGTCGAAGACGTCTCGACGACGCGTACGGTACCGCGCGGCACGCTGCGCATTTCGAGCAGTTTCGGTTTCGGCCG
GCACGTCGTCGCGCCCGCGCTGCTCGACTTCAGCGCGCGCTATCCGCAGCTGAACGTGCGGCTCGATCTGTTCGACCGGC
TCGTCGACGTGGCGGCCGAGGGATACGACCTCGACGTGCGGATCGGCGACGAGATCGCCGATCATCTGATCGCGCGTCGC
CTCGCCGCGAACTACCGCGTGCTATGTGCGTCGCCCGACTATCTCGCGCGGCGCGGCATGCCGCGCACGCTCGCGGATCT
CGCGTCGCACGACTGTCTTGCGATCAAGGAGCGCGATCATCCGTTCGGCGTATGGCGATTGAACGTGCGCGGCGATACGG
TGACCGTGAAGGTCGGCGGCGCGTTGTCGACGAATCACGGCGAAGTGGCCGTGCAATGGGCGCTGGCCGGACGCGGCATC
GTGCTGCGCTCGATCTGGGAAGCGGGGCCGCTGATCGACAGCGGCGCGCTGTGCCGCGTGCTGCCCGATGCGGTGCAGCC
GGCGAACATCTGGGCGGTCTATACGGAACGCGTGGCCGCGTCGGCGAAGGTGCGCGTGTGCGTCGATTTTCTCGCGCAGG
CGCTGGCCGGACTCAATGCCGACTCGGCTGCCGGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GACGACCGCCGGCACCATGCGCGGCGGCAACGTCTCGTCATTCTATTTATTTCCATCCGGATTAGAATCGTGCGAAACGT
TAATCCACTTTCCACGAGTC

Downstream 100 bases:

>100_bases
CGCCGCGCGACGCGGCCCAAAGGTGAGCGTTTACGGGAGGTCGAATCGCGCGTCGCGAACCGGCTGAACATCGCGCCAAC
GTGGGCGGCATGGGCGCGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNKSPNLEDLRVFSLVVRLASFSAAAEQLAVSPAYVSKRIAMLETQLGTRLLHRSTRRVTVTEAGERVFAHAEKILDDVD
HLVEDVSTTRTVPRGTLRISSSFGFGRHVVAPALLDFSARYPQLNVRLDLFDRLVDVAAEGYDLDVRIGDEIADHLIARR
LAANYRVLCASPDYLARRGMPRTLADLASHDCLAIKERDHPFGVWRLNVRGDTVTVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLIDSGALCRVLPDAVQPANIWAVYTERVAASAKVRVCVDFLAQALAGLNADSAAGAA

Sequences:

>Translated_307_residues
MNKSPNLEDLRVFSLVVRLASFSAAAEQLAVSPAYVSKRIAMLETQLGTRLLHRSTRRVTVTEAGERVFAHAEKILDDVD
HLVEDVSTTRTVPRGTLRISSSFGFGRHVVAPALLDFSARYPQLNVRLDLFDRLVDVAAEGYDLDVRIGDEIADHLIARR
LAANYRVLCASPDYLARRGMPRTLADLASHDCLAIKERDHPFGVWRLNVRGDTVTVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLIDSGALCRVLPDAVQPANIWAVYTERVAASAKVRVCVDFLAQALAGLNADSAAGAA
>Mature_307_residues
MNKSPNLEDLRVFSLVVRLASFSAAAEQLAVSPAYVSKRIAMLETQLGTRLLHRSTRRVTVTEAGERVFAHAEKILDDVD
HLVEDVSTTRTVPRGTLRISSSFGFGRHVVAPALLDFSARYPQLNVRLDLFDRLVDVAAEGYDLDVRIGDEIADHLIARR
LAANYRVLCASPDYLARRGMPRTLADLASHDCLAIKERDHPFGVWRLNVRGDTVTVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLIDSGALCRVLPDAVQPANIWAVYTERVAASAKVRVCVDFLAQALAGLNADSAAGAA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=58.7837837837838, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=48.972602739726, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=35.0694444444444, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=33.9160839160839, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=32.9861111111111, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=28.6206896551724, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=29.0102389078498, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789173, Length=268, Percent_Identity=29.4776119402985, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788706, Length=194, Percent_Identity=29.8969072164948, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786448, Length=257, Percent_Identity=26.8482490272374, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786713, Length=272, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI157672245, Length=120, Percent_Identity=32.5, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=133, Percent_Identity=32.3308270676692, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33356; Mature: 33356

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSPNLEDLRVFSLVVRLASFSAAAEQLAVSPAYVSKRIAMLETQLGTRLLHRSTRRVT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VTEAGERVFAHAEKILDDVDHLVEDVSTTRTVPRGTLRISSSFGFGRHVVAPALLDFSAR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCC
YPQLNVRLDLFDRLVDVAAEGYDLDVRIGDEIADHLIARRLAANYRVLCASPDYLARRGM
CCEEEEEHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCEEEECCCHHHHHCCC
PRTLADLASHDCLAIKERDHPFGVWRLNVRGDTVTVKVGGALSTNHGEVAVQWALAGRGI
CHHHHHHCCCCEEEEECCCCCEEEEEEEECCCEEEEEECCEEECCCCCEEEEEEECCCCH
VLRSIWEAGPLIDSGALCRVLPDAVQPANIWAVYTERVAASAKVRVCVDFLAQALAGLNA
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
DSAAGAA
CCCCCCC
>Mature Secondary Structure
MNKSPNLEDLRVFSLVVRLASFSAAAEQLAVSPAYVSKRIAMLETQLGTRLLHRSTRRVT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VTEAGERVFAHAEKILDDVDHLVEDVSTTRTVPRGTLRISSSFGFGRHVVAPALLDFSAR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCC
YPQLNVRLDLFDRLVDVAAEGYDLDVRIGDEIADHLIARRLAANYRVLCASPDYLARRGM
CCEEEEEHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCEEEECCCHHHHHCCC
PRTLADLASHDCLAIKERDHPFGVWRLNVRGDTVTVKVGGALSTNHGEVAVQWALAGRGI
CHHHHHHCCCCEEEEECCCCCEEEEEEEECCCEEEEEECCEEECCCCCEEEEEEECCCCH
VLRSIWEAGPLIDSGALCRVLPDAVQPANIWAVYTERVAASAKVRVCVDFLAQALAGLNA
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
DSAAGAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]