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The map label for this gene is nth [H]

Identifier: 189184853

GI number: 189184853

Start: 1981262

End: 1981900

Strand: Direct

Name: nth [H]

Synonym: OTT_1946

Alternate gene names: 189184853

Gene position: 1981262-1981900 (Clockwise)

Preceding gene: 189184850

Following gene: 189184854

Centisome position: 98.62

GC content: 31.92

Gene sequence:

>639_bases
ATGATAATTGATGGGATTGAAAAAATTTTTTCAAAATTTGCTGAACGATGCCCTGATCCAAAAACAGAACTAGAATATTG
CAATCATTTTACGTTGTTGGTTGCTGTTATACTTTCAGCTCAGTCTACTGATAATGCTGTTAATAAGGCTACTAAGGAGC
TATTTAAATATTATAAAACTCCTGAACAGTTTTTGCAGCTAGGAGAAGAAAATTTAAAAAAGCATATTAAGTCTATTGGA
CTATATAATAATAAAGCTAAAAATATAATTAAACTTTCTGAAATTCTTGTCAAAGAGTATAATGGGCAAGTTCCAAACAC
TATGAAAGAGCTTGAAGCACTACCTGGCGTAGGTAGAAAAAGCGCTAATGTAGTACTAAGTTGCGCTTTTGGAGTAGCAA
CTATGCCAGTAGATACTCATGTTTTTCGAGTTGCTAAAAGGATAGGTTTAGCCACTGGAGCTACTCCATTAAAAGTTGAA
AGTGAATTGCTATCAGTTATTCCAGATAGATGGCTACTATTAGCACATCATTGGTTAGTATTACATGGTAGATATATATG
TAAAGCTCAAACTCCAAAATGTTCAGAATGTTTTCTTAATAATTATTGTCAATATTTTTTATCAGGACAGAAAAAATGA

Upstream 100 bases:

>100_bases
CTGAAAAATATTATCTTTATTCATAGGAATTATATACAATTATTACCAACTTCAAAATATTATATAAAACTTTAAAGGTA
AAGTTAAGAGGAATTATAAT

Downstream 100 bases:

>100_bases
GTCAGCTAGAAATTACATCAAAGGAATCTGAAACTAAACAATTAATAGTAATGTTACATGGAGTAGGATCTAATGGTCAT
GATTTAATAAGTATTGCTCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIIDGIEKIFSKFAERCPDPKTELEYCNHFTLLVAVILSAQSTDNAVNKATKELFKYYKTPEQFLQLGEENLKKHIKSIG
LYNNKAKNIIKLSEILVKEYNGQVPNTMKELEALPGVGRKSANVVLSCAFGVATMPVDTHVFRVAKRIGLATGATPLKVE
SELLSVIPDRWLLLAHHWLVLHGRYICKAQTPKCSECFLNNYCQYFLSGQKK

Sequences:

>Translated_212_residues
MIIDGIEKIFSKFAERCPDPKTELEYCNHFTLLVAVILSAQSTDNAVNKATKELFKYYKTPEQFLQLGEENLKKHIKSIG
LYNNKAKNIIKLSEILVKEYNGQVPNTMKELEALPGVGRKSANVVLSCAFGVATMPVDTHVFRVAKRIGLATGATPLKVE
SELLSVIPDRWLLLAHHWLVLHGRYICKAQTPKCSECFLNNYCQYFLSGQKK
>Mature_212_residues
MIIDGIEKIFSKFAERCPDPKTELEYCNHFTLLVAVILSAQSTDNAVNKATKELFKYYKTPEQFLQLGEENLKKHIKSIG
LYNNKAKNIIKLSEILVKEYNGQVPNTMKELEALPGVGRKSANVVLSCAFGVATMPVDTHVFRVAKRIGLATGATPLKVE
SELLSVIPDRWLLLAHHWLVLHGRYICKAQTPKCSECFLNNYCQYFLSGQKK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=31.8435754189944, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1787920, Length=198, Percent_Identity=51.010101010101, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1789331, Length=127, Percent_Identity=33.8582677165354, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.7683615819209, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319304, Length=202, Percent_Identity=29.2079207920792, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324530, Length=207, Percent_Identity=25.6038647342995, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550361, Length=194, Percent_Identity=31.4432989690722, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23919; Mature: 23919

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIDGIEKIFSKFAERCPDPKTELEYCNHFTLLVAVILSAQSTDNAVNKATKELFKYYKT
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
PEQFLQLGEENLKKHIKSIGLYNNKAKNIIKLSEILVKEYNGQVPNTMKELEALPGVGRK
HHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
SANVVLSCAFGVATMPVDTHVFRVAKRIGLATGATPLKVESELLSVIPDRWLLLAHHWLV
CHHHHEEHHHCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
LHGRYICKAQTPKCSECFLNNYCQYFLSGQKK
HHCCCEEECCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIIDGIEKIFSKFAERCPDPKTELEYCNHFTLLVAVILSAQSTDNAVNKATKELFKYYKT
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
PEQFLQLGEENLKKHIKSIGLYNNKAKNIIKLSEILVKEYNGQVPNTMKELEALPGVGRK
HHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
SANVVLSCAFGVATMPVDTHVFRVAKRIGLATGATPLKVESELLSVIPDRWLLLAHHWLV
CHHHHEEHHHCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
LHGRYICKAQTPKCSECFLNNYCQYFLSGQKK
HHCCCEEECCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]