Click here to switch to the map view.

The map label for this gene is traD [H]

Identifier: 189184345

GI number: 189184345

Start: 1468849

End: 1470384

Strand: Direct

Name: traD [H]

Synonym: OTT_1438

Alternate gene names: 189184345

Gene position: 1468849-1470384 (Clockwise)

Preceding gene: 189184344

Following gene: 189184347

Centisome position: 73.11

GC content: 33.27

Gene sequence:

>1536_bases
GTGGTTACAATATATCAAATAGGCATTAAGTTTTATAGTTTAAGGTTTAAAAAATGGATGCATCTCAATGCTGAAGACTT
TCTGCATGAGTTTTATACAGGTCAACATGGATTTAAAATACAGCAATTATGCGAATTCCTAATCAATTCAGCATTATTAG
AAAGCTTCATTGTTTTTGCTATTGGTGTGATAATCTCAATTGTTTTCTTTACAGCTCAAGGTAAAAAAACGATTATTAAG
GCCAAAATTAGAGGTGCTGATTTTGTAGGATACAAATGCTTAGCCAAAATGCTAAAAAGCGCTAAAAAGGCCTCGAAAAT
CCGTTTTGGAGGCTTGCCATTAGTAAAGAATAGTGAAAGATTACATATTCTGATTACTGGAACAACAGGTACTGGTAAAA
CTAATATGCTTAACGAACTGCTACCACAAATTCGATTACACAAAGATCGAGCAATCATTGTAGACACTACTGGAGCTTTT
ACTGATAGATTTTTTGACCCTAAATGTGATAAACTGCTTAATCCTTTTGAAAAAAATAGTGAGCAATGGTTGCCTTGGAA
TGATTGTTTTGAAGCAGCTGATTTTCATGATATAGCTAGTAGTTTTAGTAATTATACTCATAAAATTGATGACTTTTTTA
CTAAAAATGCTGAATTAGTTTTGTCTGAAGCGTTGAAGCTATATCAGGACGATAAAGATATCATAAAACTAATTCATACA
ATCATTTACTCTGATAATAGACAATTTGCAAAAGCTTTTAGAAACACAGCTGTATCAGGTATTATAAGCGAAAGCGCGCT
CGAAACTTCTGCAGGAATTCAATCTACTCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGGAGGTAGTTTTA
GCATCAAGGAATGGTTTAGTAATTCGAATGAAACTGGCTGGCTATTTATCACAGCTAACCCAAATCAAAGAGCTACTTTA
TGCCCACTTATTTCAGCATGGATAAGCATAGCTATCAAGGCTTTGATGTGTAGAAATCCTAATCATGATAACAAAAACAT
GTGGTTTATACTCGATGAATTGCCAGCTCTACAAAAAGTTTCATCTTTACCAGTTGCTTTAGCTGAAAGTAGAAAGTATG
GAGGCTGCTTTGTTGCTGGCTTGCAAAACATTCATCAATTAGAAGCAATATATGGGGCTGCTGAATGTGCTTCTATGCTG
GATTTGTTTAATAGTAAATTTATTTTTCGAGTTAGTGATCAGGTTACAGCTTATAAGTCAGCATTAACACTAGGTGAGCA
AGAAATTATTGAAACTCAAGAGAACTTGTCATATGGATCAAATACTATGCGAGATGGGGTAAATATGAATAATGTTGAGC
GTAAAAAGATTTTAGTTATGCCATCTGAAATTATGAACCTACCAGACCTTACATGTTATGTAAAACTTGCTGGTAACTTT
CCCATCACAAAACTAACTATGCAGCTACAAAACTTAAATACAGCTTTTGTTTGTGAATATAAATTGCTCAAAAAACTTAA
GTTAGTAGAGTATTAA

Upstream 100 bases:

>100_bases
GGAGAAATGCCTGCTCACATGATGCCTATTTTACAATCTGTTTTTTTTGCATTAGTAGTTTCAATGATCTTTATTGTATT
TCCAATGGGCTTACTACCTG

Downstream 100 bases:

>100_bases
TTCGAAAAATTAATACTCTATGTTATTTTTTAAATTAATCACTTCTTCTTTTGACAATCCAGTATTTTCAGAAATAAATT
CAACTGAAAAGCCAGCTTTT

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVTIYQIGIKFYSLRFKKWMHLNAEDFLHEFYTGQHGFKIQQLCEFLINSALLESFIVFAIGVIISIVFFTAQGKKTIIK
AKIRGADFVGYKCLAKMLKSAKKASKIRFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAF
TDRFFDPKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYQDDKDIIKLIHT
IIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPNQRATL
CPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASML
DLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNF
PITKLTMQLQNLNTAFVCEYKLLKKLKLVEY

Sequences:

>Translated_511_residues
MVTIYQIGIKFYSLRFKKWMHLNAEDFLHEFYTGQHGFKIQQLCEFLINSALLESFIVFAIGVIISIVFFTAQGKKTIIK
AKIRGADFVGYKCLAKMLKSAKKASKIRFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAF
TDRFFDPKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYQDDKDIIKLIHT
IIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPNQRATL
CPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASML
DLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNF
PITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
>Mature_511_residues
MVTIYQIGIKFYSLRFKKWMHLNAEDFLHEFYTGQHGFKIQQLCEFLINSALLESFIVFAIGVIISIVFFTAQGKKTIIK
AKIRGADFVGYKCLAKMLKSAKKASKIRFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAF
TDRFFDPKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYQDDKDIIKLIHT
IIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPNQRATL
CPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASML
DLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNF
PITKLTMQLQNLNTAFVCEYKLLKKLKLVEY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 57784; Mature: 57784

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTIYQIGIKFYSLRFKKWMHLNAEDFLHEFYTGQHGFKIQQLCEFLINSALLESFIVFA
CEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGVIISIVFFTAQGKKTIIKAKIRGADFVGYKCLAKMLKSAKKASKIRFGGLPLVKNSER
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEECCCCEEECCCE
LHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDPKCDKLLNPFEKNS
EEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHHHCCCCCCHHHHCCHHHCCC
EQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYQDDKDIIKLIHT
CCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCHHHHHC
NSNETGWLFITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKV
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
SSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQVTAYKS
HHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHH
ALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNF
HHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECHHHCCCCCCEEEEEEECCC
PITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
CHHHHHHHHHCCCEEEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MVTIYQIGIKFYSLRFKKWMHLNAEDFLHEFYTGQHGFKIQQLCEFLINSALLESFIVFA
CEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGVIISIVFFTAQGKKTIIKAKIRGADFVGYKCLAKMLKSAKKASKIRFGGLPLVKNSER
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEECCCCEEECCCE
LHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDPKCDKLLNPFEKNS
EEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHHHCCCCCCHHHHCCHHHCCC
EQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLYQDDKDIIKLIHT
CCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHH
IIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCCCCCCHHHHHC
NSNETGWLFITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKV
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHH
SSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKFIFRVSDQVTAYKS
HHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHH
ALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNF
HHHCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECHHHCCCCCCEEEEEEECCC
PITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
CHHHHHHHHHCCCEEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]