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The map label for this gene is recJ [H]

Identifier: 189184334

GI number: 189184334

Start: 1461221

End: 1462984

Strand: Direct

Name: recJ [H]

Synonym: OTT_1427

Alternate gene names: 189184334

Gene position: 1461221-1462984 (Clockwise)

Preceding gene: 189184333

Following gene: 189184338

Centisome position: 72.73

GC content: 32.31

Gene sequence:

>1764_bases
ATGAATACCGTGAATGAACAATCTGTACTTGGCAATTATTGGAATAAAACTGAGTTTGATGAGTATATCATAGAAACTAT
CAGGCAAAAACATGGAGTAAGTGATTTTTTAGCAAAATTATTATATGCACAAAATATTGATTTAGAGCAAATATCATACT
TTTTAACTCCAACTATTAAGGAAGCTTTACCTGATCCTTTTCATTTACTTGATATGGATAAGGCAGTTAATAGAGTTATT
CACGCAATTAACCAGCAGGAGCATATATGCATCTTTGGAGATTATGATGTTGATGGAGCTACTTCTTCAGCTTTATTGAA
AAACTATTTTTGCGAATTAGGCATAGAACCAATTATATATATACCTGAAAGACTTACTGAAGGTTATGGACCATCAGTAT
CAGCTATTAAAAAGCTTAAGTCTGCTGGTATTAGTTTAATTATTACAGTAGATTGTGGTACTAGTGCGTACGAAGCTTTA
GCTGAAGCTAAAGCACAACATATAGATGTTATCGTTATTGATCATCATTTAAGTGTTGAGCAGTTGCCTGATGCTGTTGC
AATAATAAATCCTAATCGGTTAGATGAAACAAGCAAATATAACTATCTTGCAGCAGTTGGAGTATCATTTCTATTTTTAG
TAGCTCTTAGATCTGCCTTGCGTAAGCTAAATTATTTCGCTGATAAATCTGAACCAAGTTTAGTTAAATATTTAGACTTA
GTAGCACTTGGTACAGTTTGTGATGTTATGGCATTAATTGGTTTAAATAGAGCGTTCGTAACTAAAGGATTGGAGATAAT
GGCTCAGCATCAAAATGTTGGTATAAAAGCATTAGGTTATCTTAGTGAAGCAGAATTTTCTTCTGACTGTTACTATTTAG
GCTTTGTTTTAGGACCCAGAATAAATGCAGGTGGACGTATTGGAAAATCAGATCTAGGTAGTAAATTATTATCTACAAAA
TGTCAGATAGAGGCATACAATATTGCCTCTGAATTAGAAAATTATAATTCTGAACGAAAATTAATAGAAAAAGTTATGCT
TGAAGAAGCAGTAACTATAGCTCGTGAACAAGCCGATAATAATGTAATTGTTGTTGCTAATCATAAATGGCATCCTGGAG
TGATTGGTATTATTGCTAGCAGATTAAAAGATTTATTCCATAAGCCAATAGTTGTTATAAGTTTAGATGATACTTTAGGA
CGTGCATCATGCAGATCGATTAAGGGAATAGATATTGGCAGCAAAATTGCGGAAGCAAAGCTGAAAGGATTGCTAATTAC
TGGCGGCGGACATGCTATGGCTGCTGGATTTACCATAAAACGCGATCTTATTGGTGATTTAAAAAATTTTTTAAATCAAG
AATTAGCACTTGATATGAAAAATTTAACTGGTCATAACATTAGAAATTATGCTATAGATCTTTCAATTGCAAGTTTGAAC
ATTAACACTGTTCATGAAATTCAAAAACTTGCTCCATTTGGTAATGGCAATCCAGAGCCAATATTTAAGCTAACTAATAT
TAAATTAAAATATGCAAGCCAATTGTCTAACAAACATGTTCACTGTGTTTTATCTGAGTGGGAAAATATTAATGCAGTTA
AAACAACTAAAGCAATTGCTTTTAACCCTAGAACTCAGCTATTTGATGCTTTGTTAAGCACTTATACTAAACCAATTGAT
CTAATAGGTAATATCAAGGTTAATCGTTGGCGTGGTAGAGAAGAAATACAATTTGTAATAATGGACTTGCTTATCAATAG
ATAA

Upstream 100 bases:

>100_bases
CGTAATGCCATAGATGATAGAGTTGCTGCAAGTATTATACTTGAAGGCACATTAAAACGAATGCAGAATTCTAAAAGTTA
TTTTTAAGAAAACATAAACT

Downstream 100 bases:

>100_bases
TTTACTCTATATATTAAGGAAGTATTCTACTTTTTTGAATTTAATAGTACAAGAAGAGGTATATCTCTATTACTAGTTAA
ACCGTATAAATATTCTATGT

Product: single-stranded-DNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNTVNEQSVLGNYWNKTEFDEYIIETIRQKHGVSDFLAKLLYAQNIDLEQISYFLTPTIKEALPDPFHLLDMDKAVNRVI
HAINQQEHICIFGDYDVDGATSSALLKNYFCELGIEPIIYIPERLTEGYGPSVSAIKKLKSAGISLIITVDCGTSAYEAL
AEAKAQHIDVIVIDHHLSVEQLPDAVAIINPNRLDETSKYNYLAAVGVSFLFLVALRSALRKLNYFADKSEPSLVKYLDL
VALGTVCDVMALIGLNRAFVTKGLEIMAQHQNVGIKALGYLSEAEFSSDCYYLGFVLGPRINAGGRIGKSDLGSKLLSTK
CQIEAYNIASELENYNSERKLIEKVMLEEAVTIAREQADNNVIVVANHKWHPGVIGIIASRLKDLFHKPIVVISLDDTLG
RASCRSIKGIDIGSKIAEAKLKGLLITGGGHAMAAGFTIKRDLIGDLKNFLNQELALDMKNLTGHNIRNYAIDLSIASLN
INTVHEIQKLAPFGNGNPEPIFKLTNIKLKYASQLSNKHVHCVLSEWENINAVKTTKAIAFNPRTQLFDALLSTYTKPID
LIGNIKVNRWRGREEIQFVIMDLLINR

Sequences:

>Translated_587_residues
MNTVNEQSVLGNYWNKTEFDEYIIETIRQKHGVSDFLAKLLYAQNIDLEQISYFLTPTIKEALPDPFHLLDMDKAVNRVI
HAINQQEHICIFGDYDVDGATSSALLKNYFCELGIEPIIYIPERLTEGYGPSVSAIKKLKSAGISLIITVDCGTSAYEAL
AEAKAQHIDVIVIDHHLSVEQLPDAVAIINPNRLDETSKYNYLAAVGVSFLFLVALRSALRKLNYFADKSEPSLVKYLDL
VALGTVCDVMALIGLNRAFVTKGLEIMAQHQNVGIKALGYLSEAEFSSDCYYLGFVLGPRINAGGRIGKSDLGSKLLSTK
CQIEAYNIASELENYNSERKLIEKVMLEEAVTIAREQADNNVIVVANHKWHPGVIGIIASRLKDLFHKPIVVISLDDTLG
RASCRSIKGIDIGSKIAEAKLKGLLITGGGHAMAAGFTIKRDLIGDLKNFLNQELALDMKNLTGHNIRNYAIDLSIASLN
INTVHEIQKLAPFGNGNPEPIFKLTNIKLKYASQLSNKHVHCVLSEWENINAVKTTKAIAFNPRTQLFDALLSTYTKPID
LIGNIKVNRWRGREEIQFVIMDLLINR
>Mature_587_residues
MNTVNEQSVLGNYWNKTEFDEYIIETIRQKHGVSDFLAKLLYAQNIDLEQISYFLTPTIKEALPDPFHLLDMDKAVNRVI
HAINQQEHICIFGDYDVDGATSSALLKNYFCELGIEPIIYIPERLTEGYGPSVSAIKKLKSAGISLIITVDCGTSAYEAL
AEAKAQHIDVIVIDHHLSVEQLPDAVAIINPNRLDETSKYNYLAAVGVSFLFLVALRSALRKLNYFADKSEPSLVKYLDL
VALGTVCDVMALIGLNRAFVTKGLEIMAQHQNVGIKALGYLSEAEFSSDCYYLGFVLGPRINAGGRIGKSDLGSKLLSTK
CQIEAYNIASELENYNSERKLIEKVMLEEAVTIAREQADNNVIVVANHKWHPGVIGIIASRLKDLFHKPIVVISLDDTLG
RASCRSIKGIDIGSKIAEAKLKGLLITGGGHAMAAGFTIKRDLIGDLKNFLNQELALDMKNLTGHNIRNYAIDLSIASLN
INTVHEIQKLAPFGNGNPEPIFKLTNIKLKYASQLSNKHVHCVLSEWENINAVKTTKAIAFNPRTQLFDALLSTYTKPID
LIGNIKVNRWRGREEIQFVIMDLLINR

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=542, Percent_Identity=33.3948339483395, Blast_Score=251, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 65155; Mature: 65155

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVNEQSVLGNYWNKTEFDEYIIETIRQKHGVSDFLAKLLYAQNIDLEQISYFLTPTIK
CCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCHHH
EALPDPFHLLDMDKAVNRVIHAINQQEHICIFGDYDVDGATSSALLKNYFCELGIEPIIY
HHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEE
IPERLTEGYGPSVSAIKKLKSAGISLIITVDCGTSAYEALAEAKAQHIDVIVIDHHLSVE
CCHHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCHH
QLPDAVAIINPNRLDETSKYNYLAAVGVSFLFLVALRSALRKLNYFADKSEPSLVKYLDL
HCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VALGTVCDVMALIGLNRAFVTKGLEIMAQHQNVGIKALGYLSEAEFSSDCYYLGFVLGPR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCEEEEEEECCC
INAGGRIGKSDLGSKLLSTKCQIEAYNIASELENYNSERKLIEKVMLEEAVTIAREQADN
CCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
NVIVVANHKWHPGVIGIIASRLKDLFHKPIVVISLDDTLGRASCRSIKGIDIGSKIAEAK
CEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCCCCCCCCHHHHHHH
LKGLLITGGGHAMAAGFTIKRDLIGDLKNFLNQELALDMKNLTGHNIRNYAIDLSIASLN
HCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
INTVHEIQKLAPFGNGNPEPIFKLTNIKLKYASQLSNKHVHCVLSEWENINAVKTTKAIA
CHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEHHHCCCCCEEEEEECCCCCCEEEECEEEE
FNPRTQLFDALLSTYTKPIDLIGNIKVNRWRGREEIQFVIMDLLINR
ECCHHHHHHHHHHHCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTVNEQSVLGNYWNKTEFDEYIIETIRQKHGVSDFLAKLLYAQNIDLEQISYFLTPTIK
CCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCHHH
EALPDPFHLLDMDKAVNRVIHAINQQEHICIFGDYDVDGATSSALLKNYFCELGIEPIIY
HHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEE
IPERLTEGYGPSVSAIKKLKSAGISLIITVDCGTSAYEALAEAKAQHIDVIVIDHHLSVE
CCHHHCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCHH
QLPDAVAIINPNRLDETSKYNYLAAVGVSFLFLVALRSALRKLNYFADKSEPSLVKYLDL
HCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VALGTVCDVMALIGLNRAFVTKGLEIMAQHQNVGIKALGYLSEAEFSSDCYYLGFVLGPR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCEEEEEEECCC
INAGGRIGKSDLGSKLLSTKCQIEAYNIASELENYNSERKLIEKVMLEEAVTIAREQADN
CCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
NVIVVANHKWHPGVIGIIASRLKDLFHKPIVVISLDDTLGRASCRSIKGIDIGSKIAEAK
CEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCCCCCCCCHHHHHHH
LKGLLITGGGHAMAAGFTIKRDLIGDLKNFLNQELALDMKNLTGHNIRNYAIDLSIASLN
HCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
INTVHEIQKLAPFGNGNPEPIFKLTNIKLKYASQLSNKHVHCVLSEWENINAVKTTKAIA
CHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEHHHCCCCCEEEEEECCCCCCEEEECEEEE
FNPRTQLFDALLSTYTKPIDLIGNIKVNRWRGREEIQFVIMDLLINR
ECCHHHHHHHHHHHCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]