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The map label for this gene is tpiA [H]

Identifier: 189184245

GI number: 189184245

Start: 1362877

End: 1363626

Strand: Direct

Name: tpiA [H]

Synonym: OTT_1338

Alternate gene names: 189184245

Gene position: 1362877-1363626 (Clockwise)

Preceding gene: 189184243

Following gene: 189184246

Centisome position: 67.84

GC content: 29.07

Gene sequence:

>750_bases
ATGGAATCAATTGTAGTTTCTAATTGGAAAATGCACTTTAGTTTTTCTGAAGCTTGCAATTACCTAAATTTAATAACTAG
CTTAAATAGCAATTTAAATCTTGCTAAGATGATATTTGCTGTACCAAATTTATATTTATCTGGGCTTAAATTAAAATTTA
ATGACACATATCATTTTTCAGCTCAAGATGTAAGCATGATTACAGAAAGTAGTGGCCCATATACTGGTGAGATAAGTGCT
AGTATGTTGAAAAACTTAAATGTGAACTATGCTATAGTAGGGCACTCAGAACGAAGGCTATTATTTTATGAGGATGCTAA
TACAATAGCATTAAAAGTAAGAAATTGTATCAATAATGCAATAGTCCCAATTGTATGTATAGGAGAACCAATTGAAGCAC
GAAAAAATAAAACATATCTTCAATATCTAGCTCAACAGTTAAGTAGTATATCTTTTAGTTTTACTAAGAATGTTATCATT
GCTTATGAGCCAATATGGTCAATTGGCAGTGATATGATTCCTACCATTGATGATATTTATGAGGTAGTAACTATGATTAG
AGAAATACAAAATAGGTATATACCTCATAATATTGAAAATAGTGTTAAAATAGTATATGGTGGTTCTGTTTCAGCTAATA
ATATTCACCAAATTCTAACAGCTGGCGTTGATGGAGTATTAATTGGTAAGGCATCTTTAAAATTAGAAAGTTTAACTACA
ATAATAAAAACAGTTCAAGGTTTAGATTAA

Upstream 100 bases:

>100_bases
TGTTCTTAATTTATTTAACACTGTAACTTGTATAATATATGTATATACCAATACAATATTTAAGTATTATAAATCTGCAA
GTAATTTTTTATCTTAAAGT

Downstream 100 bases:

>100_bases
ATAAAATGATGAATATACTACTTTTTATACACATTGTAATATCAGTATTATTAGTTACAGCAATATTACTTCACAAAACT
GGAGCAGATAGTTTAAGTGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MESIVVSNWKMHFSFSEACNYLNLITSLNSNLNLAKMIFAVPNLYLSGLKLKFNDTYHFSAQDVSMITESSGPYTGEISA
SMLKNLNVNYAIVGHSERRLLFYEDANTIALKVRNCINNAIVPIVCIGEPIEARKNKTYLQYLAQQLSSISFSFTKNVII
AYEPIWSIGSDMIPTIDDIYEVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNIHQILTAGVDGVLIGKASLKLESLTT
IIKTVQGLD

Sequences:

>Translated_249_residues
MESIVVSNWKMHFSFSEACNYLNLITSLNSNLNLAKMIFAVPNLYLSGLKLKFNDTYHFSAQDVSMITESSGPYTGEISA
SMLKNLNVNYAIVGHSERRLLFYEDANTIALKVRNCINNAIVPIVCIGEPIEARKNKTYLQYLAQQLSSISFSFTKNVII
AYEPIWSIGSDMIPTIDDIYEVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNIHQILTAGVDGVLIGKASLKLESLTT
IIKTVQGLD
>Mature_249_residues
MESIVVSNWKMHFSFSEACNYLNLITSLNSNLNLAKMIFAVPNLYLSGLKLKFNDTYHFSAQDVSMITESSGPYTGEISA
SMLKNLNVNYAIVGHSERRLLFYEDANTIALKVRNCINNAIVPIVCIGEPIEARKNKTYLQYLAQQLSSISFSFTKNVII
AYEPIWSIGSDMIPTIDDIYEVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNIHQILTAGVDGVLIGKASLKLESLTT
IIKTVQGLD

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=237, Percent_Identity=30.8016877637131, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI226529917, Length=238, Percent_Identity=30.672268907563, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1790353, Length=256, Percent_Identity=31.25, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17536593, Length=236, Percent_Identity=31.7796610169492, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320255, Length=171, Percent_Identity=35.0877192982456, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28572008, Length=235, Percent_Identity=31.9148936170213, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28572006, Length=235, Percent_Identity=31.9148936170213, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28572004, Length=235, Percent_Identity=31.9148936170213, Blast_Score=126, Evalue=1e-29,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27753; Mature: 27753

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIVVSNWKMHFSFSEACNYLNLITSLNSNLNLAKMIFAVPNLYLSGLKLKFNDTYHFS
CCCEEEECEEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCEEEEC
AQDVSMITESSGPYTGEISASMLKNLNVNYAIVGHSERRLLFYEDANTIALKVRNCINNA
CCHHHEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEEHHHHCCC
IVPIVCIGEPIEARKNKTYLQYLAQQLSSISFSFTKNVIIAYEPIWSIGSDMIPTIDDIY
CEEEEEECCCHHHHCCHHHHHHHHHHHHHCEEEEECCEEEEECCHHHCCCCCCCCHHHHH
EVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNIHQILTAGVDGVLIGKASLKLESLTT
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHH
IIKTVQGLD
HHHHHHCCC
>Mature Secondary Structure
MESIVVSNWKMHFSFSEACNYLNLITSLNSNLNLAKMIFAVPNLYLSGLKLKFNDTYHFS
CCCEEEECEEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCEEEEC
AQDVSMITESSGPYTGEISASMLKNLNVNYAIVGHSERRLLFYEDANTIALKVRNCINNA
CCHHHEEECCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEEHHHHCCC
IVPIVCIGEPIEARKNKTYLQYLAQQLSSISFSFTKNVIIAYEPIWSIGSDMIPTIDDIY
CEEEEEECCCHHHHCCHHHHHHHHHHHHHCEEEEECCEEEEECCHHHCCCCCCCCHHHHH
EVVTMIREIQNRYIPHNIENSVKIVYGGSVSANNIHQILTAGVDGVLIGKASLKLESLTT
HHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHH
IIKTVQGLD
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA