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The map label for this gene is rnhA [H]

Identifier: 189184157

GI number: 189184157

Start: 1281529

End: 1282035

Strand: Reverse

Name: rnhA [H]

Synonym: OTT_1250

Alternate gene names: 189184157

Gene position: 1282035-1281529 (Counterclockwise)

Preceding gene: 189184158

Following gene: 189184156

Centisome position: 63.81

GC content: 32.15

Gene sequence:

>507_bases
GTGATTGACTATGCTTGCTCTGCTACTAATAACATTCCAGTTAAGATATATACAGATGGTGCGTGCAGCGGTAATCCAGG
ACCAGGAGGTTGGGGTGTATATTTAAGTTATAAGAATCATGAAAGAAAAGTTTATGGATCTAACATAAATACTACAAATA
ATAGAATGGAGTTAACAGCAACAATAGAGGCGTTGCGGCTTCTTAAGTATAGCTGTAAAGTAGAATTATATACTGATAGT
CAGTATGTTCAAATGGGTATTACTAAATGGATAAAAAAGTGGATAAAAAATAATTGGGAAACAAGCAATAAGCAATTGGT
TTGTAATGTTGATTTATGGCAGTCGTTATATACTTTGATTCAAAAACACCAAGTTAGTTGGCATTGGGTGAGAGGACACT
CTGGCAATATAGGTAATGAAATTGCTGATAAATTAGCAACTAATGGAAAAATTGAAGCAATGAAAATAGTTAAAAATAAT
GAAAATACTAAGAAATTTCTTGATTAG

Upstream 100 bases:

>100_bases
ATTGCGATCAGTTAGTAGCCACAAATGAAAAGTTGAAAAATATGAAAGACATAGTAGGAAATAAAGTTGTTAGTCTCGAC
AGCTTTAGGAAGAAAATCTA

Downstream 100 bases:

>100_bases
ATATACAAGTAAGTTTGTTGGTGTTGACCAATATGGTAATAATTATTATATTTCTTTAATGAAAAGTAATCATTTAAAGA
ATAGAAAGAGAATAATAATT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MIDYACSATNNIPVKIYTDGACSGNPGPGGWGVYLSYKNHERKVYGSNINTTNNRMELTATIEALRLLKYSCKVELYTDS
QYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQSLYTLIQKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIVKNN
ENTKKFLD

Sequences:

>Translated_168_residues
MIDYACSATNNIPVKIYTDGACSGNPGPGGWGVYLSYKNHERKVYGSNINTTNNRMELTATIEALRLLKYSCKVELYTDS
QYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQSLYTLIQKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIVKNN
ENTKKFLD
>Mature_168_residues
MIDYACSATNNIPVKIYTDGACSGNPGPGGWGVYLSYKNHERKVYGSNINTTNNRMELTATIEALRLLKYSCKVELYTDS
QYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQSLYTLIQKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIVKNN
ENTKKFLD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=41.2587412587413, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1786408, Length=133, Percent_Identity=53.3834586466165, Blast_Score=152, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI115532542, Length=147, Percent_Identity=41.4965986394558, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=39.4557823129252, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=39.4557823129252, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 19222; Mature: 19222

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYACSATNNIPVKIYTDGACSGNPGPGGWGVYLSYKNHERKVYGSNINTTNNRMELTA
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCEEEEHH
TIEALRLLKYSCKVELYTDSQYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQSLYTLI
HHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHH
QKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIVKNNENTKKFLD
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHCCC
>Mature Secondary Structure
MIDYACSATNNIPVKIYTDGACSGNPGPGGWGVYLSYKNHERKVYGSNINTTNNRMELTA
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCEEEEHH
TIEALRLLKYSCKVELYTDSQYVQMGITKWIKKWIKNNWETSNKQLVCNVDLWQSLYTLI
HHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHH
QKHQVSWHWVRGHSGNIGNEIADKLATNGKIEAMKIVKNNENTKKFLD
HHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA