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The map label for this gene is hisS [H]

Identifier: 189184154

GI number: 189184154

Start: 1279431

End: 1280702

Strand: Reverse

Name: hisS [H]

Synonym: OTT_1247

Alternate gene names: 189184154

Gene position: 1280702-1279431 (Counterclockwise)

Preceding gene: 189184155

Following gene: 189184153

Centisome position: 63.75

GC content: 31.53

Gene sequence:

>1272_bases
ATGTCAATTAAATTGCAGCCAGTTAAGGGTAGCAAAGATTTACTACCTGAAGAATTTGGAAAACATGACTATATTGTTAG
CGTTTCTAGGAATTTAAGTCAGTTATATGGATTTCAACCTATATCTACTCCAATCATAGAATACACAGAAATATTTAATC
GCACTTTAGGCAAAGACTCAGATGTTCTTAGTAAAGAGATGTATGTTTTTCTTGATAAAGGAAATCGTAGTGTTAGTTTA
AGGCCAGAGTTTACTGCAAGTATTATGCGGGCAGTTATTTATAATAATCTACAAAATAAAAAACTACCATTAAAATATTT
TTCATCAGGACCAGCATTTAGATATGATAATCCTCAAGCTGGTAGACAGCGTCAATTTCACCAAATTAATTTGGAGTGTA
TTGGTGATGATTCTCCATTCAGTGATGCAGAAATAGTGTTGCTAGCTCATGATATATTAAAAACATTAAATTTAGTTGAT
AAAGTGAACTTAGAAATAAATTCACTTGGCTGCATGGAATCAAGAGCAAAATATCAACAAGCTTTGGTTGAGTATTTTAG
TAAATATAGAACTGAATTATCACAAGATAGTCAAAGTCGTCTTATTGAAAATCCACTGCGAATTTTAGATTCCAAAAATT
TACATGATAAAAAAATATCTGCTTCAGCGCCTAGCATACATGATTATTACACTATTAAATCTAGATGTTACTTTGATAAA
GTGCTAGAATATTTAGAATTTTGTGGTATTAAATATACTATAAATGCTAATTTAGTGCGCGGGTTAGATTATTACTGTCA
TACAGTTTTTGAGTATACATCAACTCAAATTGGAGCTCAATCTACTGTTTTGGGAGGAGGGAGATATGATGGACTATTCG
AACAAATGGGAGGCAAATTAGCAAGTGGCAAAAAAACGTTGCCTGCTATCGGTTTTGCTGCTGGAATAGAAAGATTAGCA
CTTTTAACTAATTATACTCCAGTGGATGTAAGACCTATAGTTATTATACCAGTAGATGAGGAACATCACCAACACGGTAT
TATGTTGCTGCAAAAATTAAGAAATAATAATATTACAACTGTAATTGATTTGCAGGACAGCATTTCAAAAAGATTAAATC
GAGCTAATTACATTAAGGCAAGTAAGGTAATATTTATTGGAGCAATCGAAGTGCGAGAACAAAGTTATAGAATCAAAGAC
TTAGATACTAGTAATGAATGTGTAATAATACATAATGAACTACTTAGCTATTTGCAAAATAACTGCTCTTAA

Upstream 100 bases:

>100_bases
TTACACAATCAGCTATAAATGTTGAAAGAATGATTGGCAAAGTATTCTCTGAACTTTTTGGCAGCAACTATTTTAAAATC
TCTTCTAGCAGGCATTAAAA

Downstream 100 bases:

>100_bases
TATCAAATTGGTTGTTCTATTATATGATTAAGATTAATACTAAGACTATAACTAGAATTGCAAGTATTCAAGGAGTTTAT
AGTTATCAAATTTTGGACTC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSIKLQPVKGSKDLLPEEFGKHDYIVSVSRNLSQLYGFQPISTPIIEYTEIFNRTLGKDSDVLSKEMYVFLDKGNRSVSL
RPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQAGRQRQFHQINLECIGDDSPFSDAEIVLLAHDILKTLNLVD
KVNLEINSLGCMESRAKYQQALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIKSRCYFDK
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYTSTQIGAQSTVLGGGRYDGLFEQMGGKLASGKKTLPAIGFAAGIERLA
LLTNYTPVDVRPIVIIPVDEEHHQHGIMLLQKLRNNNITTVIDLQDSISKRLNRANYIKASKVIFIGAIEVREQSYRIKD
LDTSNECVIIHNELLSYLQNNCS

Sequences:

>Translated_423_residues
MSIKLQPVKGSKDLLPEEFGKHDYIVSVSRNLSQLYGFQPISTPIIEYTEIFNRTLGKDSDVLSKEMYVFLDKGNRSVSL
RPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQAGRQRQFHQINLECIGDDSPFSDAEIVLLAHDILKTLNLVD
KVNLEINSLGCMESRAKYQQALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIKSRCYFDK
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYTSTQIGAQSTVLGGGRYDGLFEQMGGKLASGKKTLPAIGFAAGIERLA
LLTNYTPVDVRPIVIIPVDEEHHQHGIMLLQKLRNNNITTVIDLQDSISKRLNRANYIKASKVIFIGAIEVREQSYRIKD
LDTSNECVIIHNELLSYLQNNCS
>Mature_422_residues
SIKLQPVKGSKDLLPEEFGKHDYIVSVSRNLSQLYGFQPISTPIIEYTEIFNRTLGKDSDVLSKEMYVFLDKGNRSVSLR
PEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQAGRQRQFHQINLECIGDDSPFSDAEIVLLAHDILKTLNLVDK
VNLEINSLGCMESRAKYQQALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIKSRCYFDKV
LEYLEFCGIKYTINANLVRGLDYYCHTVFEYTSTQIGAQSTVLGGGRYDGLFEQMGGKLASGKKTLPAIGFAAGIERLAL
LTNYTPVDVRPIVIIPVDEEHHQHGIMLLQKLRNNNITTVIDLQDSISKRLNRANYIKASKVIFIGAIEVREQSYRIKDL
DTSNECVIIHNELLSYLQNNCS

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=352, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788861, Length=430, Percent_Identity=36.2790697674419, Blast_Score=286, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24643061, Length=371, Percent_Identity=25.0673854447439, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24643059, Length=390, Percent_Identity=23.8461538461538, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48126; Mature: 47994

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLQPVKGSKDLLPEEFGKHDYIVSVSRNLSQLYGFQPISTPIIEYTEIFNRTLGKDS
CCEEEECCCCCCCCCHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DVLSKEMYVFLDKGNRSVSLRPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQA
HHHCCEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCC
GRQRQFHQINLECIGDDSPFSDAEIVLLAHDILKTLNLVDKVNLEINSLGCMESRAKYQQ
CCCCCCEEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHH
ALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIKSRCYFDK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHH
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYTSTQIGAQSTVLGGGRYDGLFEQMGGKL
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCEE
ASGKKTLPAIGFAAGIERLALLTNYTPVDVRPIVIIPVDEEHHQHGIMLLQKLRNNNITT
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEE
VIDLQDSISKRLNRANYIKASKVIFIGAIEVREQSYRIKDLDTSNECVIIHNELLSYLQN
EEECHHHHHHHHHHHCCEEECCEEEEEEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHH
NCS
CCC
>Mature Secondary Structure 
SIKLQPVKGSKDLLPEEFGKHDYIVSVSRNLSQLYGFQPISTPIIEYTEIFNRTLGKDS
CEEEECCCCCCCCCHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DVLSKEMYVFLDKGNRSVSLRPEFTASIMRAVIYNNLQNKKLPLKYFSSGPAFRYDNPQA
HHHCCEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCC
GRQRQFHQINLECIGDDSPFSDAEIVLLAHDILKTLNLVDKVNLEINSLGCMESRAKYQQ
CCCCCCEEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHH
ALVEYFSKYRTELSQDSQSRLIENPLRILDSKNLHDKKISASAPSIHDYYTIKSRCYFDK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHH
VLEYLEFCGIKYTINANLVRGLDYYCHTVFEYTSTQIGAQSTVLGGGRYDGLFEQMGGKL
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCEE
ASGKKTLPAIGFAAGIERLALLTNYTPVDVRPIVIIPVDEEHHQHGIMLLQKLRNNNITT
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEE
VIDLQDSISKRLNRANYIKASKVIFIGAIEVREQSYRIKDLDTSNECVIIHNELLSYLQN
EEECHHHHHHHHHHHCCEEECCEEEEEEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHH
NCS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA