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The map label for this gene is xthA [H]

Identifier: 189184130

GI number: 189184130

Start: 1257316

End: 1258101

Strand: Direct

Name: xthA [H]

Synonym: OTT_1223

Alternate gene names: 189184130

Gene position: 1257316-1258101 (Clockwise)

Preceding gene: 189184129

Following gene: 189184132

Centisome position: 62.58

GC content: 32.06

Gene sequence:

>786_bases
ATGAAAATAATTACTTGGAACATTAACTCTGTTAGGATGCGTATACCTCTTTTACAAAGATTAGTTCATCTATATAATCC
AGATATTATTGCTTTACAAGAGACAAAAGTAGAAGACAGACTGTTTCCAGTAACAGAATGCATAAATTTAGGATATAAAT
ATATTACTTATAGTGGACAAAAATCTTATAATGGAGTAGCAATCTTATCCAGAATTTCATTTGAATCTACGTTTGTAATA
AATCTATATAATGGTGAAAAAAGGCATATTGCTATTAACATAGGAGAGTTAAGCATCCATAATTTTTATGTGCCTGCAGG
AGGAGATATTCCGAATGCAAATGTAAATATAAAGTTTAAGCATAAATTATGCTATGTTGATTTTATGAATGCTTGGTTTT
TGAATAATAAGTCTAAAAAAGATAAGATTATACTGCTGGGAGACCTTAATATTGCGCCATTAGAAAGTGACGTATGGTCA
AGTTATCAATTACGCAATGTTGTGTCTTATACAGATATTGAACGAAAAAAATTAATGCATAATATTACTAGCTTTGATTG
GATTGATGCAGTGCGCTTTTTTTCCGGTAAACACGAAAAATTGTATTCCTGGTGGAGCTATCGAAATGTTGACTGGAGCA
AATTCGATCGCGGCCGCAGGCTAGATCATATATGGATTAGTCATCCTTTAGAATCCAAAATTAAATCTTGTATAATCATC
AAAGATGCCAGGGGCTGGGATATGCCATCTGATCATGTTCCAGTTATGCTAGAAATAGAAACTTAA

Upstream 100 bases:

>100_bases
AGAATAGTAATTGTTGTAACGTCTTCTTGAATTATTAACATAAAAAGGTAAAAGTATGAAACAGAAAAATATAGCACAAT
AGAAAAAATTAACTTTCAAA

Downstream 100 bases:

>100_bases
ATGCTATATCAACCTACTGCAGTATACTAAGTATTTAAAAACTAAGACACAAGCTCAAGTTTATACTGAACATTGAGAAG
GCCATTCTTTAAGGACTCAA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKIITWNINSVRMRIPLLQRLVHLYNPDIIALQETKVEDRLFPVTECINLGYKYITYSGQKSYNGVAILSRISFESTFVI
NLYNGEKRHIAINIGELSIHNFYVPAGGDIPNANVNIKFKHKLCYVDFMNAWFLNNKSKKDKIILLGDLNIAPLESDVWS
SYQLRNVVSYTDIERKKLMHNITSFDWIDAVRFFSGKHEKLYSWWSYRNVDWSKFDRGRRLDHIWISHPLESKIKSCIII
KDARGWDMPSDHVPVMLEIET

Sequences:

>Translated_261_residues
MKIITWNINSVRMRIPLLQRLVHLYNPDIIALQETKVEDRLFPVTECINLGYKYITYSGQKSYNGVAILSRISFESTFVI
NLYNGEKRHIAINIGELSIHNFYVPAGGDIPNANVNIKFKHKLCYVDFMNAWFLNNKSKKDKIILLGDLNIAPLESDVWS
SYQLRNVVSYTDIERKKLMHNITSFDWIDAVRFFSGKHEKLYSWWSYRNVDWSKFDRGRRLDHIWISHPLESKIKSCIII
KDARGWDMPSDHVPVMLEIET
>Mature_261_residues
MKIITWNINSVRMRIPLLQRLVHLYNPDIIALQETKVEDRLFPVTECINLGYKYITYSGQKSYNGVAILSRISFESTFVI
NLYNGEKRHIAINIGELSIHNFYVPAGGDIPNANVNIKFKHKLCYVDFMNAWFLNNKSKKDKIILLGDLNIAPLESDVWS
SYQLRNVVSYTDIERKKLMHNITSFDWIDAVRFFSGKHEKLYSWWSYRNVDWSKFDRGRRLDHIWISHPLESKIKSCIII
KDARGWDMPSDHVPVMLEIET

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=29.5880149812734, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30673; Mature: 30673

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIITWNINSVRMRIPLLQRLVHLYNPDIIALQETKVEDRLFPVTECINLGYKYITYSGQ
CEEEEEECCCEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCCEEEEEECCC
KSYNGVAILSRISFESTFVINLYNGEKRHIAINIGELSIHNFYVPAGGDIPNANVNIKFK
CCCCCEEEEEEECCCEEEEEEEECCCCEEEEEEECEEEEEEEEECCCCCCCCCEEEEEEE
HKLCYVDFMNAWFLNNKSKKDKIILLGDLNIAPLESDVWSSYQLRNVVSYTDIERKKLMH
EEEEEEEEHHEEEECCCCCCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
NITSFDWIDAVRFFSGKHEKLYSWWSYRNVDWSKFDRGRRLDHIWISHPLESKIKSCIII
HCCCCHHHHHHHHHCCCCHHEEEECCCCCCCHHHHCCCCCEEEEEECCCHHHCCCEEEEE
KDARGWDMPSDHVPVMLEIET
ECCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKIITWNINSVRMRIPLLQRLVHLYNPDIIALQETKVEDRLFPVTECINLGYKYITYSGQ
CEEEEEECCCEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCCEEEEEECCC
KSYNGVAILSRISFESTFVINLYNGEKRHIAINIGELSIHNFYVPAGGDIPNANVNIKFK
CCCCCEEEEEEECCCEEEEEEEECCCCEEEEEEECEEEEEEEEECCCCCCCCCEEEEEEE
HKLCYVDFMNAWFLNNKSKKDKIILLGDLNIAPLESDVWSSYQLRNVVSYTDIERKKLMH
EEEEEEEEHHEEEECCCCCCCEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
NITSFDWIDAVRFFSGKHEKLYSWWSYRNVDWSKFDRGRRLDHIWISHPLESKIKSCIII
HCCCCHHHHHHHHHCCCCHHEEEECCCCCCCHHHHCCCCCEEEEEECCCHHHCCCEEEEE
KDARGWDMPSDHVPVMLEIET
ECCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]