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The map label for this gene is pleD [H]

Identifier: 189183991

GI number: 189183991

Start: 1116879

End: 1118348

Strand: Direct

Name: pleD [H]

Synonym: OTT_1084

Alternate gene names: 189183991

Gene position: 1116879-1118348 (Clockwise)

Preceding gene: 189183989

Following gene: 189183993

Centisome position: 55.59

GC content: 28.71

Gene sequence:

>1470_bases
ATGACTGCTGCAATACTTGTTGTTGATGATTTAGAATATAATAGAAAGCTTTTAGAAACAAAGTTATTAACAGAATATTA
TACTGTATACACCGCTGCTAATGGACAAGAAGCTATTAAAATACTTGAAGCTATTAAAATAGATGTTGTATTACTTGATA
TTGTGATGCCAGGTATGGATGGCTTTGAACTTTGCAAAGCCATAAAGTCCAATCAAGATACTGCTCATATACCTGTAATC
ATAATAACTGCGCTATCAGATATTGAGGATAAAGTTAAAGGTCTTGAATGTGGAGCAGATGAATTTTTAACTAAACCAAT
AGAAGATATTTCTTTATTTGCTAGAGTAAAATCATTAGTGCGTATGAAAGCAGTTTCTAATCTAATAAAACTTCGCAGCA
TAACTTACAATCAAATTAATAATAATGATTCTGAATTTCTTAACGCTAATAAGGTTGGTACTACACTTAGTTTTAAACAA
AGTAATGATTTTTCAAATAGCACAATTTTAATTATTGATGATGATGATATATGGTTCAATCGCATCTCAGAAATAATAAC
ATGCCTTACTAAAAAAGTTGAGTTATTTGAATGTATAAATTATACAGATAACAAAAATAAGTTATTAACAGATTTAGAGA
AAATAGCCACTAGCTTGGTTCCAGATTTATTAATAATTAATAACCAAATTATATTAGAAGATCCATTAAGAGTATATGCA
ATTTTACGGTCTAAAGATGAATTAAAGCAATCAGCATTTATGCTGCTAACTGAAGATTCATTGGAAAACAGAGATTATGA
TGATATTAGTACTGATAAACTACTTAAGGGATTAGAAATGGGAATAAATGATTTCTTTCCTTACTCAATTGACAATAACG
AATTACTAGCAAGAATTAAAACTCAATTAAGACGTAAACAATATCAAGATCAACTAAAGCAAGATCTAGATAATAGGTTA
AACTTAGCAGCAAAAGATGGATTAACTAATATTTTTAATCGTAGATACTTTGATAGTCATATTAATCAACTTATTAATGA
TGCTAAAGACAGTAATAAGTCACTGTTTTTAATAATGATAGACTTTGATCATTTTAAAATGGTAAATGACATTTATGGAC
ACCAAGTAGGTGATGAAGTATTAAAGGCTTCTGTCAAAATTATAGAGACTAATATCAGAGTTACTGACTTAATTGCAAGA
TATGGTGGGGAAGAGTTTATAATTACTATCTACGACGATGATATTGATCAGCAAAAGGCAATAGCATTGGCAAATAGAAT
TAAAATTAAAATTGAAACACACAATTTTATCATTTCTAATCTAAAACTTCAACTTAAGCAAACTATATCAATAGGAATCA
CTGAGTACGATCCACCAGAAGATGCTAATGCTTTAATTAGCAGAACTGACAAAGCGCTTTATAATGCCAAACATTATGGT
AGAAATCAAGTATGTGCTCTATTTAGGTAA

Upstream 100 bases:

>100_bases
ATTATATTTATTATCGTAATATTTATTATATAGTTAATGTAGTAGAATGATAGTACCAATAGTGATATCATGATCCAATG
CCATTATTTCTTTCTGTAGT

Downstream 100 bases:

>100_bases
AATTAAGTGTAACAATTAACCAAAGTTAACTATAAGTTATATATCAGAAAGCATTAAGATTAAGTAAAAGATTAAACTTT
GTATAATGTTGATTTTGAGT

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTAAILVVDDLEYNRKLLETKLLTEYYTVYTAANGQEAIKILEAIKIDVVLLDIVMPGMDGFELCKAIKSNQDTAHIPVI
IITALSDIEDKVKGLECGADEFLTKPIEDISLFARVKSLVRMKAVSNLIKLRSITYNQINNNDSEFLNANKVGTTLSFKQ
SNDFSNSTILIIDDDDIWFNRISEIITCLTKKVELFECINYTDNKNKLLTDLEKIATSLVPDLLIINNQIILEDPLRVYA
ILRSKDELKQSAFMLLTEDSLENRDYDDISTDKLLKGLEMGINDFFPYSIDNNELLARIKTQLRRKQYQDQLKQDLDNRL
NLAAKDGLTNIFNRRYFDSHINQLINDAKDSNKSLFLIMIDFDHFKMVNDIYGHQVGDEVLKASVKIIETNIRVTDLIAR
YGGEEFIITIYDDDIDQQKAIALANRIKIKIETHNFIISNLKLQLKQTISIGITEYDPPEDANALISRTDKALYNAKHYG
RNQVCALFR

Sequences:

>Translated_489_residues
MTAAILVVDDLEYNRKLLETKLLTEYYTVYTAANGQEAIKILEAIKIDVVLLDIVMPGMDGFELCKAIKSNQDTAHIPVI
IITALSDIEDKVKGLECGADEFLTKPIEDISLFARVKSLVRMKAVSNLIKLRSITYNQINNNDSEFLNANKVGTTLSFKQ
SNDFSNSTILIIDDDDIWFNRISEIITCLTKKVELFECINYTDNKNKLLTDLEKIATSLVPDLLIINNQIILEDPLRVYA
ILRSKDELKQSAFMLLTEDSLENRDYDDISTDKLLKGLEMGINDFFPYSIDNNELLARIKTQLRRKQYQDQLKQDLDNRL
NLAAKDGLTNIFNRRYFDSHINQLINDAKDSNKSLFLIMIDFDHFKMVNDIYGHQVGDEVLKASVKIIETNIRVTDLIAR
YGGEEFIITIYDDDIDQQKAIALANRIKIKIETHNFIISNLKLQLKQTISIGITEYDPPEDANALISRTDKALYNAKHYG
RNQVCALFR
>Mature_488_residues
TAAILVVDDLEYNRKLLETKLLTEYYTVYTAANGQEAIKILEAIKIDVVLLDIVMPGMDGFELCKAIKSNQDTAHIPVII
ITALSDIEDKVKGLECGADEFLTKPIEDISLFARVKSLVRMKAVSNLIKLRSITYNQINNNDSEFLNANKVGTTLSFKQS
NDFSNSTILIIDDDDIWFNRISEIITCLTKKVELFECINYTDNKNKLLTDLEKIATSLVPDLLIINNQIILEDPLRVYAI
LRSKDELKQSAFMLLTEDSLENRDYDDISTDKLLKGLEMGINDFFPYSIDNNELLARIKTQLRRKQYQDQLKQDLDNRLN
LAAKDGLTNIFNRRYFDSHINQLINDAKDSNKSLFLIMIDFDHFKMVNDIYGHQVGDEVLKASVKIIETNIRVTDLIARY
GGEEFIITIYDDDIDQQKAIALANRIKIKIETHNFIISNLKLQLKQTISIGITEYDPPEDANALISRTDKALYNAKHYGR
NQVCALFR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=43.9759036144578, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=37.1584699453552, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=30.6748466257669, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=33.9622641509434, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=31.5151515151515, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=32.7044025157233, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=34.6820809248555, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=34.1666666666667, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788956, Length=204, Percent_Identity=32.3529411764706, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786599, Length=145, Percent_Identity=31.7241379310345, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=38.8888888888889, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=31.9277108433735, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786784, Length=120, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=42.7184466019417, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=34.1666666666667, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=29.2682926829268, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082012, Length=144, Percent_Identity=27.0833333333333, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=41.025641025641, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=32.7731092436975, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788905, Length=112, Percent_Identity=39.2857142857143, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787487, Length=116, Percent_Identity=31.8965517241379, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=172, Percent_Identity=30.8139534883721, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786911, Length=135, Percent_Identity=28.8888888888889, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI2367329, Length=139, Percent_Identity=34.5323741007194, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790552, Length=120, Percent_Identity=35, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789402, Length=104, Percent_Identity=31.7307692307692, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081977, Length=156, Percent_Identity=29.4871794871795, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081974, Length=107, Percent_Identity=32.7102803738318, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55933; Mature: 55802

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAILVVDDLEYNRKLLETKLLTEYYTVYTAANGQEAIKILEAIKIDVVLLDIVMPGMD
CCEEEEEEECCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHEEEEHHCCCCC
GFELCKAIKSNQDTAHIPVIIITALSDIEDKVKGLECGADEFLTKPIEDISLFARVKSLV
HHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHH
RMKAVSNLIKLRSITYNQINNNDSEFLNANKVGTTLSFKQSNDFSNSTILIIDDDDIWFN
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHH
RISEIITCLTKKVELFECINYTDNKNKLLTDLEKIATSLVPDLLIINNQIILEDPLRVYA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEEECCHHHHH
ILRSKDELKQSAFMLLTEDSLENRDYDDISTDKLLKGLEMGINDFFPYSIDNNELLARIK
HHHCHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
TQLRRKQYQDQLKQDLDNRLNLAAKDGLTNIFNRRYFDSHINQLINDAKDSNKSLFLIMI
HHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DFDHFKMVNDIYGHQVGDEVLKASVKIIETNIRVTDLIARYGGEEFIITIYDDDIDQQKA
ECHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHH
IALANRIKIKIETHNFIISNLKLQLKQTISIGITEYDPPEDANALISRTDKALYNAKHYG
HHHHEEEEEEEEECEEEEEHHHHEEEHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
RNQVCALFR
CCCEEEECC
>Mature Secondary Structure 
TAAILVVDDLEYNRKLLETKLLTEYYTVYTAANGQEAIKILEAIKIDVVLLDIVMPGMD
CEEEEEEECCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHEEEEHHCCCCC
GFELCKAIKSNQDTAHIPVIIITALSDIEDKVKGLECGADEFLTKPIEDISLFARVKSLV
HHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHH
RMKAVSNLIKLRSITYNQINNNDSEFLNANKVGTTLSFKQSNDFSNSTILIIDDDDIWFN
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCHHHH
RISEIITCLTKKVELFECINYTDNKNKLLTDLEKIATSLVPDLLIINNQIILEDPLRVYA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCEEEEECCHHHHH
ILRSKDELKQSAFMLLTEDSLENRDYDDISTDKLLKGLEMGINDFFPYSIDNNELLARIK
HHHCHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
TQLRRKQYQDQLKQDLDNRLNLAAKDGLTNIFNRRYFDSHINQLINDAKDSNKSLFLIMI
HHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DFDHFKMVNDIYGHQVGDEVLKASVKIIETNIRVTDLIARYGGEEFIITIYDDDIDQQKA
ECHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHH
IALANRIKIKIETHNFIISNLKLQLKQTISIGITEYDPPEDANALISRTDKALYNAKHYG
HHHHEEEEEEEEECEEEEEHHHHEEEHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
RNQVCALFR
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA