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The map label for this gene is rlmB [H]

Identifier: 189183948

GI number: 189183948

Start: 1081478

End: 1082278

Strand: Reverse

Name: rlmB [H]

Synonym: OTT_1041

Alternate gene names: 189183948

Gene position: 1082278-1081478 (Counterclockwise)

Preceding gene: 189183980

Following gene: 189183947

Centisome position: 53.87

GC content: 28.59

Gene sequence:

>801_bases
TTGATTCTGTCAAGTGATTATTTAATAATCGTCAATTATTACAAAATATTTATTTTGTCCAATAAATTTATTATGACTGA
TAAAAAATCATATTATATATTTGGTACTCATGCATCCTTAGCAGCTTTAAATAACCCTCTCAGGGTAATAAGGTATATTT
ATTGTACAGAGTCATGTTTTCTATCTTTATCAAAATTGATTCAAAAGCATGATTATAAAATAGTTACAACAAAATTTCTA
CATCAGCTATTAGGAAGCCAAATAGTTCATCAAGGAATAGCAGTAAATACCATACCACTCACAATTAATGGTATCAAACA
TATAGACCTAGCTACACCAAACTATAAAATTGCTATTTTAGACCAATTGCTAGACCATAATAACTTAGGAGCTATTATAA
GATCAGCTGCAGCATTTAATATATCAACTATTATCCTACCTGAAAATAACTCTATAGAAGAGAACGGCACTATTGCTAAG
ATAGCAGTTGGAGCTCTAGAAAAAATAAACTTAGTAAAAGTAAAAAATTTATATAATACTATCAATTACTTAAAAAAGTA
TGGATTTTGGATTATCGGGCTAGATCTTAACAACAGTAATCAACTTACAGAGCAAACTTTTCATTCAAAAATGGCTTTTG
TTTTAGGCTCTGAGAATAAAGGACTAAGGAACTTAACACGCCAACAATGTGATTTTTTAATCAAAATTCCAATGTCTCAT
AAAACTGAAAGTTTAAACGTTGCAGGAGCTGCTACAATAATTTTTTATGAGAGCTTCAAATTTTCCATAAAACAACAATA
A

Upstream 100 bases:

>100_bases
TAGCTAGGGTATCTTAAAAATAAACAAATCATACTAGCAAAGCTTAATACACAATTTTGCTTAACTAAAAACATGATTTA
GTACTTTCTATAATAATTAC

Downstream 100 bases:

>100_bases
TTAAATAACAAGAAAATAATTTTAATAATTCAATAAATACTATTATAATTCTACGAACACTAAAAATTTATTATTTTTAA
ATAATCAGGAAATATCATGT

Product: tRNA/rRNA methyltransferase, TrmH family

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MILSSDYLIIVNYYKIFILSNKFIMTDKKSYYIFGTHASLAALNNPLRVIRYIYCTESCFLSLSKLIQKHDYKIVTTKFL
HQLLGSQIVHQGIAVNTIPLTINGIKHIDLATPNYKIAILDQLLDHNNLGAIIRSAAAFNISTIILPENNSIEENGTIAK
IAVGALEKINLVKVKNLYNTINYLKKYGFWIIGLDLNNSNQLTEQTFHSKMAFVLGSENKGLRNLTRQQCDFLIKIPMSH
KTESLNVAGAATIIFYESFKFSIKQQ

Sequences:

>Translated_266_residues
MILSSDYLIIVNYYKIFILSNKFIMTDKKSYYIFGTHASLAALNNPLRVIRYIYCTESCFLSLSKLIQKHDYKIVTTKFL
HQLLGSQIVHQGIAVNTIPLTINGIKHIDLATPNYKIAILDQLLDHNNLGAIIRSAAAFNISTIILPENNSIEENGTIAK
IAVGALEKINLVKVKNLYNTINYLKKYGFWIIGLDLNNSNQLTEQTFHSKMAFVLGSENKGLRNLTRQQCDFLIKIPMSH
KTESLNVAGAATIIFYESFKFSIKQQ
>Mature_266_residues
MILSSDYLIIVNYYKIFILSNKFIMTDKKSYYIFGTHASLAALNNPLRVIRYIYCTESCFLSLSKLIQKHDYKIVTTKFL
HQLLGSQIVHQGIAVNTIPLTINGIKHIDLATPNYKIAILDQLLDHNNLGAIIRSAAAFNISTIILPENNSIEENGTIAK
IAVGALEKINLVKVKNLYNTINYLKKYGFWIIGLDLNNSNQLTEQTFHSKMAFVLGSENKGLRNLTRQQCDFLIKIPMSH
KTESLNVAGAATIIFYESFKFSIKQQ

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=175, Percent_Identity=35.4285714285714, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30112; Mature: 30112

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSSDYLIIVNYYKIFILSNKFIMTDKKSYYIFGTHASLAALNNPLRVIRYIYCTESCF
CEECCCCEEEEEEEEEEEEECEEEEECCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHH
LSLSKLIQKHDYKIVTTKFLHQLLGSQIVHQGIAVNTIPLTINGIKHIDLATPNYKIAIL
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCEEEEEEEEEECCEEEEEECCCCEEEEEE
DQLLDHNNLGAIIRSAAAFNISTIILPENNSIEENGTIAKIAVGALEKINLVKVKNLYNT
HHHHCCCCHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEECCCHHHCEEEEHHHHHHH
INYLKKYGFWIIGLDLNNSNQLTEQTFHSKMAFVLGSENKGLRNLTRQQCDFLIKIPMSH
HHHHHHCCCEEEEEEECCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCEEEECCCCC
KTESLNVAGAATIIFYESFKFSIKQQ
CCCCEEECCEEEEEEEEEEEEEEECC
>Mature Secondary Structure
MILSSDYLIIVNYYKIFILSNKFIMTDKKSYYIFGTHASLAALNNPLRVIRYIYCTESCF
CEECCCCEEEEEEEEEEEEECEEEEECCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHH
LSLSKLIQKHDYKIVTTKFLHQLLGSQIVHQGIAVNTIPLTINGIKHIDLATPNYKIAIL
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCEEEEEEEEEECCEEEEEECCCCEEEEEE
DQLLDHNNLGAIIRSAAAFNISTIILPENNSIEENGTIAKIAVGALEKINLVKVKNLYNT
HHHHCCCCHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEECCCHHHCEEEEHHHHHHH
INYLKKYGFWIIGLDLNNSNQLTEQTFHSKMAFVLGSENKGLRNLTRQQCDFLIKIPMSH
HHHHHHCCCEEEEEEECCCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHCCEEEECCCCC
KTESLNVAGAATIIFYESFKFSIKQQ
CCCCEEECCEEEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]