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The map label for this gene is ddl [H]

Identifier: 189183856

GI number: 189183856

Start: 993562

End: 994536

Strand: Direct

Name: ddl [H]

Synonym: OTT_0949

Alternate gene names: 189183856

Gene position: 993562-994536 (Clockwise)

Preceding gene: 189183855

Following gene: 189183857

Centisome position: 49.46

GC content: 31.18

Gene sequence:

>975_bases
TTGTTGGGAAAAAAAGCCTATAGTTTTCAAAGACACAATTCTGTTGTTGAAAGTATAACTAGTTCAGGCAAAAAGCATGT
AGTAATCTTATATGGTGGAATGTCAGCAGAAAGAGAGGTGTCATTGATGTCTAATGATAATGTACAAGCTGCTCTTATCT
CTAATGGATATCAGGTAACTCGTATAGATGTTGGGCAAGATATTGCAGTTAAACTATCTGAAATTACACGACCATATACA
GTATTTAATTGTCTAGTTGGTACATATGGAGAGGATGGATGTATTCCTGGGTTATTAAATATTATGAATATACCTTATAC
TCATTCTGGTGTAAAAACTTCTGCAGCTGCATTTGATAAGCAAATTGCTAAAACTATATTACAATGCTATAAAATTAAAA
CTCCTGCCAGTATAACTATCAATAGTAATGATAATGTAATCGATGATCCTATTTTGAGACCATATGTTATAAAGCCACTA
CAGCAGGGGTCAAGTATAGGTGTAAAAATTGTACATTTAGAAGATAAATTTCTATTTAAGGATTATAAATTTCAATTTGG
TAATAAGGTTTTAGTTGAGCAATATATCAAAGGTAGGGAGCTACAGGTTGCTTTATTAGATGGTCAAGTAGTTGGAATAT
TAGAAATTAAAATGCTTAAAGATAAAATCTTTTATGATTATCAAAGTAAATATAGCCCTGGCTTTGCTGAGCACATTATT
CCACCTATGCTTCCTGTTAATACTGTCAATCAAGTTATAAACATTGCAGAAAAAGTGTATAAAATTTTTGACTGTAGGGG
GCCATGTAGATTGGAGTGCATATTATCTGATATTGACAATGAAGTTTATGTTATAGAATTAAATACTCATCCTGGCATGA
CATCTTTATCAAGTTATCCTGAAATTGCACAATATTATGGTATTAGCTTCAATGAATTAGTAGAAAGAATATTAATTACA
GCCAAATGTGATTGA

Upstream 100 bases:

>100_bases
AATGGAAATGCTACCGCTAGCGATATTGAAAATCTAGGGGAGTTGATTCGTAAAAAGGTTTTTGACCATACTGGTATAAC
ATTAGAATGGGAGATTAAAA

Downstream 100 bases:

>100_bases
ATTAAATAAAATGTACAACAAAAGTACTACTACAATAAATCAAACAAAAAAAGTTAAAAGAATCAATATTCCTTTAAGGA
GATCTATTATTAACTATTAT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLGKKAYSFQRHNSVVESITSSGKKHVVILYGGMSAEREVSLMSNDNVQAALISNGYQVTRIDVGQDIAVKLSEITRPYT
VFNCLVGTYGEDGCIPGLLNIMNIPYTHSGVKTSAAAFDKQIAKTILQCYKIKTPASITINSNDNVIDDPILRPYVIKPL
QQGSSIGVKIVHLEDKFLFKDYKFQFGNKVLVEQYIKGRELQVALLDGQVVGILEIKMLKDKIFYDYQSKYSPGFAEHII
PPMLPVNTVNQVINIAEKVYKIFDCRGPCRLECILSDIDNEVYVIELNTHPGMTSLSSYPEIAQYYGISFNELVERILIT
AKCD

Sequences:

>Translated_324_residues
MLGKKAYSFQRHNSVVESITSSGKKHVVILYGGMSAEREVSLMSNDNVQAALISNGYQVTRIDVGQDIAVKLSEITRPYT
VFNCLVGTYGEDGCIPGLLNIMNIPYTHSGVKTSAAAFDKQIAKTILQCYKIKTPASITINSNDNVIDDPILRPYVIKPL
QQGSSIGVKIVHLEDKFLFKDYKFQFGNKVLVEQYIKGRELQVALLDGQVVGILEIKMLKDKIFYDYQSKYSPGFAEHII
PPMLPVNTVNQVINIAEKVYKIFDCRGPCRLECILSDIDNEVYVIELNTHPGMTSLSSYPEIAQYYGISFNELVERILIT
AKCD
>Mature_324_residues
MLGKKAYSFQRHNSVVESITSSGKKHVVILYGGMSAEREVSLMSNDNVQAALISNGYQVTRIDVGQDIAVKLSEITRPYT
VFNCLVGTYGEDGCIPGLLNIMNIPYTHSGVKTSAAAFDKQIAKTILQCYKIKTPASITINSNDNVIDDPILRPYVIKPL
QQGSSIGVKIVHLEDKFLFKDYKFQFGNKVLVEQYIKGRELQVALLDGQVVGILEIKMLKDKIFYDYQSKYSPGFAEHII
PPMLPVNTVNQVINIAEKVYKIFDCRGPCRLECILSDIDNEVYVIELNTHPGMTSLSSYPEIAQYYGISFNELVERILIT
AKCD

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=304, Percent_Identity=32.5657894736842, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=29.3103448275862, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 36253; Mature: 36253

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGKKAYSFQRHNSVVESITSSGKKHVVILYGGMSAEREVSLMSNDNVQAALISNGYQVT
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCEEEE
RIDVGQDIAVKLSEITRPYTVFNCLVGTYGEDGCIPGLLNIMNIPYTHSGVKTSAAAFDK
EEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHH
QIAKTILQCYKIKTPASITINSNDNVIDDPILRPYVIKPLQQGSSIGVKIVHLEDKFLFK
HHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCEEEE
DYKFQFGNKVLVEQYIKGRELQVALLDGQVVGILEIKMLKDKIFYDYQSKYSPGFAEHII
HHHHHCCCCHHHHHHHCCCEEEEEEECCCEEEEEEEEEHHHHHHCCHHCCCCCCHHHHCC
PPMLPVNTVNQVINIAEKVYKIFDCRGPCRLECILSDIDNEVYVIELNTHPGMTSLSSYP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHCCH
EIAQYYGISFNELVERILITAKCD
HHHHHHCCCHHHHHHHHHEEECCC
>Mature Secondary Structure
MLGKKAYSFQRHNSVVESITSSGKKHVVILYGGMSAEREVSLMSNDNVQAALISNGYQVT
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCEEEE
RIDVGQDIAVKLSEITRPYTVFNCLVGTYGEDGCIPGLLNIMNIPYTHSGVKTSAAAFDK
EEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHH
QIAKTILQCYKIKTPASITINSNDNVIDDPILRPYVIKPLQQGSSIGVKIVHLEDKFLFK
HHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCEEEE
DYKFQFGNKVLVEQYIKGRELQVALLDGQVVGILEIKMLKDKIFYDYQSKYSPGFAEHII
HHHHHCCCCHHHHHHHCCCEEEEEEECCCEEEEEEEEEHHHHHHCCHHCCCCCCHHHHCC
PPMLPVNTVNQVINIAEKVYKIFDCRGPCRLECILSDIDNEVYVIELNTHPGMTSLSSYP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHCCH
EIAQYYGISFNELVERILITAKCD
HHHHHHCCCHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA