Click here to switch to the map view.

The map label for this gene is traD [H]

Identifier: 189183777

GI number: 189183777

Start: 913661

End: 914935

Strand: Direct

Name: traD [H]

Synonym: OTT_0870

Alternate gene names: 189183777

Gene position: 913661-914935 (Clockwise)

Preceding gene: 189183776

Following gene: 189183779

Centisome position: 45.48

GC content: 33.1

Gene sequence:

>1275_bases
TTGTACGGAGTAAAAAATCTAGCCAAAATGCTAAAAAGCGCTAAAAAGGCCTCGAAAATCTGTTTTGGAGGCTTGCCATT
AGTAAAGAATAGTGAAAGATTACATATTCTGATTACTGGAACAACAGGTACTGGTAAAACTAATATGCTTAACGAACTGC
TACCACAAATTCGATTACACAAAGATCGAGCAATCATTGTAGACACTACTGGAGCTTTTACTGATAGATTTTTTGACTCT
AAATGTGATAAACTGCTTAATCCTTTGGAAAAAAATAGTGAACAATGGTTGCCTTGGAATGATTGTTTTGAAGCAGCTGA
TTTTCATGATATAGCGAGTAGTTTTAGTAATTATACTCCTAAACTTGATGACTTTTTTGCTAAAAATGCTGAATTAGTCT
TGTCTGAAGCATTGAAGCTATATAAGGATGATAAAGATATCATAAAATTAATTCATACAATCATTTACTCTGATAATAGA
CAATTTGCAAAAGCTTTTAGAAACACTGCTGTATCAGGTATTATAAGCGAAAGCGCGCTCGAAACTTCTGCAGGAATTCA
ATCTACTCTTGGAAAGAATATTACTTCGCTACAATATTTAAAGCCTGGAGGTAGTTTTAGCATCAAGAAATGGTTTAGTA
ATTCAAATGAAATTGAATGGTTATTTATCACAGCTAACCCAAATCAAAGAGCTACTTTATGCCCACTTATTTCAGCATGG
ATAAGCATAGCTATCAAGGCTTTGATGTGTAGAAATCCTAATCATGATAACAAAAACATGTGGTTTATACTTGATGAACT
GCCAGCTCTACAAAAAGTTTCGTCTTTACCAGTTGCTTTAGCTGAAAGTAGAAAGTATGGAGGCTGCTTTGTCGCTGGAT
TGCAGAACATTCATCAATTAGAAGCAATATATGGGGCTGCTGAATGTGCTTCTATGCTGGATTTGTTTAATAGTAAATTT
ATTTTTCGAGTTAGTGATCAGGTTACAGCTTATAAGTCAGCATTAACACTAGGTGAGCAAGAAATTATTGAAACTCAAGA
AAACTTGTCATATGGATCAAATACTATGCGAGATGGAGTAAATATGAATAATGTTGAGCGTAAAAAGATTTTAGTTATGC
CATCTGAAATTATGAACCTACCAGACTTAACTTGTTATGTAAAACTTGCTGGTAACTTTCCTATCACAAAACTAACTATG
CAGCTACAAAACTTAAATACAGCTTTTGTTTGTGAATATAAATTGCTCAAAAAACTTAAGTTAGTAGAGTATTAA

Upstream 100 bases:

>100_bases
GGATTAATTGTTTTTGCTATTGGTGTGATAATCTCAATTGTTTTCTTTACAGCTCAAGGCAAAAACACGATTATTAAGGC
CAAAATTAGAGGCGCTGATT

Downstream 100 bases:

>100_bases
TTCGAAAAATTAATACTCTATGTTATTTTTTAAATTAACCACTTCTTTTTTTGATAACCCAGTATTTTCAGAAATAAATT
CAACTGAAAAGCCAGCTTTT

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MYGVKNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNR
QFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSNEIEWLFITANPNQRATLCPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKF
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTM
QLQNLNTAFVCEYKLLKKLKLVEY

Sequences:

>Translated_424_residues
MYGVKNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNR
QFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSNEIEWLFITANPNQRATLCPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKF
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTM
QLQNLNTAFVCEYKLLKKLKLVEY
>Mature_424_residues
MYGVKNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLHKDRAIIVDTTGAFTDRFFDS
KCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTPKLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNR
QFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSNEIEWLFITANPNQRATLCPLISAW
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLEAIYGAAECASMLDLFNSKF
IFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTM
QLQNLNTAFVCEYKLLKKLKLVEY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 47636; Mature: 47636

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGVKNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLH
CCCHHHHHHHHHHHHHHHHHEECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHEEC
KDRAIIVDTTGAFTDRFFDSKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTP
CCCEEEEECCCCHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHCCCC
KLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESAL
CHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSNEIEWLFITANPNQRATLCPLISAW
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHH
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQL
HHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHH
EAIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCC
NMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLK
CCCCCCCCEEEEECHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHH
LVEY
HCCC
>Mature Secondary Structure
MYGVKNLAKMLKSAKKASKICFGGLPLVKNSERLHILITGTTGTGKTNMLNELLPQIRLH
CCCHHHHHHHHHHHHHHHHHEECCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHEEC
KDRAIIVDTTGAFTDRFFDSKCDKLLNPLEKNSEQWLPWNDCFEAADFHDIASSFSNYTP
CCCEEEEECCCCHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHCCCC
KLDDFFAKNAELVLSEALKLYKDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESAL
CHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ETSAGIQSTLGKNITSLQYLKPGGSFSIKKWFSNSNEIEWLFITANPNQRATLCPLISAW
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHH
ISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQL
HHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHH
EAIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCC
NMNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLK
CCCCCCCCEEEEECHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHH
LVEY
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]