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The map label for this gene is metS [H]

Identifier: 189183674

GI number: 189183674

Start: 795294

End: 796829

Strand: Reverse

Name: metS [H]

Synonym: OTT_0767

Alternate gene names: 189183674

Gene position: 796829-795294 (Counterclockwise)

Preceding gene: 189183690

Following gene: 189183673

Centisome position: 39.66

GC content: 30.53

Gene sequence:

>1536_bases
ATGAAAAGCAAATATTATATTACTACTCCTATATATTATGTTAACGATATACCTCATATTGGACATGCATATACTAGCAT
TCTAGCAGATGTTTATGCTAGATTTATGAGGTTACTAGGAAAAGAAGTAATCTTTTTAACTGGTACTGATGAGCATGGTC
AAAAAGTAGAAAAGGCAGCTAGCAATGCAGGTGTTTCACCTAAAGAATTTGTTGATAAGATAGCATCATCATTTGTAAAA
CTTAGTGTTGACCTTAATTTATCAAATGATGACTTTATTAGAACAACTGATACAAAACATATTAAAGCAGTACAAAAGTT
GTGGAAGGTTCTTGAGCAACAAGGAGATATATATCTTGGTAAATATTGTGGATGGTACGCAATCAAGGACGAAGCTTTTT
ATTCAGAATCAGAGCTTACTACTGATGGTAAAGCACCAACTGGAGCTGAAGTAGAATGGGTAGAAGAATCTAGTTACTTC
TTTGCATTATCCAAATGGCAGCAAAAATTACTAGATTGGTATGAAAATAACCATGATATCATTAAACCTGCATTTTTTAG
AAATGAAGTAATCAACTTTATTAAAAATGGTCTTATTGACCTTTCGATTTCACGTACTACATTTAAATGGGGAATAGCAG
TACCTAATGATTCGTCACACGTAATATATGTTTGGCTTGATGCACTAGTAAATTATATTTCTGCATTAGGTTATGCAAGT
GATAATGGTACATTAATGACTAACTTTTGGCCAGCTGATCTTCATATAGTTGGTAAGGATATACTAAGGTTTCATGCTGT
TTATTGGCCAGCATTTCTTATGTCAGCTGGATTACCTTTGCCAAAAGCTATTTTGGTACATGGATGGTGGACTAATGAAG
GACAAAAAATTTCTAAATCAGTAGGAAATGTTATTAATCCAGTTGAATTAACAAGTAAGTTTGGAATTGATCAAGTACGT
TATTTCTTGTTACGAGAAATAACAATTGGTAACGACGGAAACTTTAACAAAATTAGTTTTATCAATAGAATTAATAGTGA
GCTATGCAATAAACTTGGTAATTTAGTCCATAGAACTTTGTCTTTTATTTATAAATATAATAAAGCAAAAATACCACAAT
TAGATGGAATAACTATTACTAATCTATACAAATCTGAATCACTCTTGTTAAAGATTGTTATGTTGAGCGACAATCTTGCT
AATATAATTGATAATGAGAATGTTACTGTTATTCTAAATAGAATCATGGAAATTGTTAATCAAGCTAATATATACTTTGA
TCAGCAAGCTCCTTGGAAGTTTAAAGATAGCAACTCTCAGAAAATAGCAACAATATTATACACATTAATTGAAACTATAA
GGTGCATAGCGATCTTATTACAGCCTTTTATACCTGAATCTGCAAATACAATTCTAGACTTAATAGCTATAGATAAAACA
GAAAGAATGTTTAGTTGTATTAATCGTTCTCATGCTATAAAACCAGGAAAAACTATTTTAGAACCAAAGCCAATTTTTGT
TAAAATTGAAGAATAA

Upstream 100 bases:

>100_bases
ATTAAAAATTATCAAGTAAAGATTTATTTGAGTTAATAAATTTATCAGCTAATCATAAAACAAATTTTCATGATATTTTG
AGATATAATATTATTATACT

Downstream 100 bases:

>100_bases
AAGAACAATATATGTTAATTGATTCGCACTGCCATTTAAATATGTTAACTGAACCAAATGCAGCAATTAAAAACGCTATA
GCTAATGGTGTTAAATCTAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKSKYYITTPIYYVNDIPHIGHAYTSILADVYARFMRLLGKEVIFLTGTDEHGQKVEKAASNAGVSPKEFVDKIASSFVK
LSVDLNLSNDDFIRTTDTKHIKAVQKLWKVLEQQGDIYLGKYCGWYAIKDEAFYSESELTTDGKAPTGAEVEWVEESSYF
FALSKWQQKLLDWYENNHDIIKPAFFRNEVINFIKNGLIDLSISRTTFKWGIAVPNDSSHVIYVWLDALVNYISALGYAS
DNGTLMTNFWPADLHIVGKDILRFHAVYWPAFLMSAGLPLPKAILVHGWWTNEGQKISKSVGNVINPVELTSKFGIDQVR
YFLLREITIGNDGNFNKISFINRINSELCNKLGNLVHRTLSFIYKYNKAKIPQLDGITITNLYKSESLLLKIVMLSDNLA
NIIDNENVTVILNRIMEIVNQANIYFDQQAPWKFKDSNSQKIATILYTLIETIRCIAILLQPFIPESANTILDLIAIDKT
ERMFSCINRSHAIKPGKTILEPKPIFVKIEE

Sequences:

>Translated_511_residues
MKSKYYITTPIYYVNDIPHIGHAYTSILADVYARFMRLLGKEVIFLTGTDEHGQKVEKAASNAGVSPKEFVDKIASSFVK
LSVDLNLSNDDFIRTTDTKHIKAVQKLWKVLEQQGDIYLGKYCGWYAIKDEAFYSESELTTDGKAPTGAEVEWVEESSYF
FALSKWQQKLLDWYENNHDIIKPAFFRNEVINFIKNGLIDLSISRTTFKWGIAVPNDSSHVIYVWLDALVNYISALGYAS
DNGTLMTNFWPADLHIVGKDILRFHAVYWPAFLMSAGLPLPKAILVHGWWTNEGQKISKSVGNVINPVELTSKFGIDQVR
YFLLREITIGNDGNFNKISFINRINSELCNKLGNLVHRTLSFIYKYNKAKIPQLDGITITNLYKSESLLLKIVMLSDNLA
NIIDNENVTVILNRIMEIVNQANIYFDQQAPWKFKDSNSQKIATILYTLIETIRCIAILLQPFIPESANTILDLIAIDKT
ERMFSCINRSHAIKPGKTILEPKPIFVKIEE
>Mature_511_residues
MKSKYYITTPIYYVNDIPHIGHAYTSILADVYARFMRLLGKEVIFLTGTDEHGQKVEKAASNAGVSPKEFVDKIASSFVK
LSVDLNLSNDDFIRTTDTKHIKAVQKLWKVLEQQGDIYLGKYCGWYAIKDEAFYSESELTTDGKAPTGAEVEWVEESSYF
FALSKWQQKLLDWYENNHDIIKPAFFRNEVINFIKNGLIDLSISRTTFKWGIAVPNDSSHVIYVWLDALVNYISALGYAS
DNGTLMTNFWPADLHIVGKDILRFHAVYWPAFLMSAGLPLPKAILVHGWWTNEGQKISKSVGNVINPVELTSKFGIDQVR
YFLLREITIGNDGNFNKISFINRINSELCNKLGNLVHRTLSFIYKYNKAKIPQLDGITITNLYKSESLLLKIVMLSDNLA
NIIDNENVTVILNRIMEIVNQANIYFDQQAPWKFKDSNSQKIATILYTLIETIRCIAILLQPFIPESANTILDLIAIDKT
ERMFSCINRSHAIKPGKTILEPKPIFVKIEE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=508, Percent_Identity=37.2047244094488, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI14043022, Length=554, Percent_Identity=24.3682310469314, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1788432, Length=506, Percent_Identity=24.901185770751, Blast_Score=175, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI212645096, Length=496, Percent_Identity=38.3064516129032, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI71992781, Length=385, Percent_Identity=35.0649350649351, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17540794, Length=565, Percent_Identity=25.3097345132743, Blast_Score=138, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321610, Length=525, Percent_Identity=36, Blast_Score=330, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6321704, Length=550, Percent_Identity=24.5454545454545, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358483, Length=515, Percent_Identity=36.1165048543689, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24655311, Length=560, Percent_Identity=25.5357142857143, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58268; Mature: 58268

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKYYITTPIYYVNDIPHIGHAYTSILADVYARFMRLLGKEVIFLTGTDEHGQKVEKAA
CCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
SNAGVSPKEFVDKIASSFVKLSVDLNLSNDDFIRTTDTKHIKAVQKLWKVLEQQGDIYLG
HCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEE
KYCGWYAIKDEAFYSESELTTDGKAPTGAEVEWVEESSYFFALSKWQQKLLDWYENNHDI
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHHCCCCCC
IKPAFFRNEVINFIKNGLIDLSISRTTFKWGIAVPNDSSHVIYVWLDALVNYISALGYAS
CCHHHHHHHHHHHHHCCCEEEEEECEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCC
DNGTLMTNFWPADLHIVGKDILRFHAVYWPAFLMSAGLPLPKAILVHGWWTNEGQKISKS
CCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHH
VGNVINPVELTSKFGIDQVRYFLLREITIGNDGNFNKISFINRINSELCNKLGNLVHRTL
HCCCCCHHHHHHHCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFIYKYNKAKIPQLDGITITNLYKSESLLLKIVMLSDNLANIIDNENVTVILNRIMEIVN
HHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHHH
QANIYFDQQAPWKFKDSNSQKIATILYTLIETIRCIAILLQPFIPESANTILDLIAIDKT
HCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
ERMFSCINRSHAIKPGKTILEPKPIFVKIEE
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKSKYYITTPIYYVNDIPHIGHAYTSILADVYARFMRLLGKEVIFLTGTDEHGQKVEKAA
CCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
SNAGVSPKEFVDKIASSFVKLSVDLNLSNDDFIRTTDTKHIKAVQKLWKVLEQQGDIYLG
HCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEE
KYCGWYAIKDEAFYSESELTTDGKAPTGAEVEWVEESSYFFALSKWQQKLLDWYENNHDI
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHHCCCCCC
IKPAFFRNEVINFIKNGLIDLSISRTTFKWGIAVPNDSSHVIYVWLDALVNYISALGYAS
CCHHHHHHHHHHHHHCCCEEEEEECEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCC
DNGTLMTNFWPADLHIVGKDILRFHAVYWPAFLMSAGLPLPKAILVHGWWTNEGQKISKS
CCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHH
VGNVINPVELTSKFGIDQVRYFLLREITIGNDGNFNKISFINRINSELCNKLGNLVHRTL
HCCCCCHHHHHHHCCHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SFIYKYNKAKIPQLDGITITNLYKSESLLLKIVMLSDNLANIIDNENVTVILNRIMEIVN
HHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHCCCCHHHHHHHHHHHHH
QANIYFDQQAPWKFKDSNSQKIATILYTLIETIRCIAILLQPFIPESANTILDLIAIDKT
HCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
ERMFSCINRSHAIKPGKTILEPKPIFVKIEE
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA