Click here to switch to the map view.

The map label for this gene is aphD [H]

Identifier: 189183454

GI number: 189183454

Start: 564558

End: 565460

Strand: Reverse

Name: aphD [H]

Synonym: OTT_0547

Alternate gene names: 189183454

Gene position: 565460-564558 (Counterclockwise)

Preceding gene: 189183455

Following gene: 189183448

Centisome position: 28.15

GC content: 37.87

Gene sequence:

>903_bases
ATGAAAACGTTTGAGCAAAACATCATTAATTTATATGGCGAAAAAGGTAAGCAGTGGTTAGATAATCTGCCGAATCTCGT
AACACAACTAGCGAACACTTATGAACTGTCAAATTTAAAACCAGTTAATAATTTAAGCTACAATTATGTGTTATCAGGTT
TTCAAGGACCTCAGCCAATTATTTTAAAATTAGGATTAGATGTTAATGGAATCAAATGTGAAGCAGCAGCCCTCATGTCA
TTTGAAGATTTTGGTGTTGTGCAAGTATTTTCAGAAAACACAGGTTTACTTCTACTTGAATGCGCAGTACCAGGCATCTC
TTTAAAGTCTTATTTTCCTAAAAACGATGATGAGGCTATTAATATCACAGCAAATGTTATCAAACGATTGCACAAAGCGC
CGATACCAAGCACCCATGCATTTCCACATATCAAAGATTGGCTTGCGGCATTAGATGGCGACATAAAATTTCCAGTGCAA
ACGCTGCAAAAAGCCAGAGAAATTCGTGATCAGCTTTTAAAAACGGCTGAGCCAGATGTTTTGCTTCATGGTGATTTACA
TCATGATAATATTTTGCAAAATTGCGAAGATTGGCTGGGGATCGATCCAAAAGGTGTAATTGGCGAACCAGCCTATGAAG
TGGCAGCTTTTATCCGCAATCCAATCCCGGAATTACTGAATCACGCTGATGCGCCAAATATTATTCATAATCGCATCACT
CTATTTGCTGAGCTCCTTGAATTACCACCGCAGCGAATCCTTGATTGGTGTTTTGTGCAAGTGGTACTTACATGGGTATG
GGCGTTAGAGGATGGTTGTGATGCAAGCTATTTTGAGAAGATCACGGGAATTTTTGATGGAATGATAAATCCTATAAAAA
AATCTCTTCAAGAAAATTCTTAA

Upstream 100 bases:

>100_bases
TAATCTGGCGTTTTTATGAAAAGCATGGTTTTTACTGCATAGGGACTATACCCAAATACCTGAAGGATTATGATCGCATT
TTCATGAGGAAAGATATCTC

Downstream 100 bases:

>100_bases
TAGATATTTCTGAGCGATGATGGGCTTTTTCATTGTATCTTACTCCAAAATCTGGAATATCTGCGCTCACAAAAGTAAAA
GCATGTTTTGCATGACCATA

Product: putative streptomycin-6-phosphotransferase

Products: NA

Alternate protein names: APH(6); Streptidine kinase; Streptomycin 6-phosphotransferase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKTFEQNIINLYGEKGKQWLDNLPNLVTQLANTYELSNLKPVNNLSYNYVLSGFQGPQPIILKLGLDVNGIKCEAAALMS
FEDFGVVQVFSENTGLLLLECAVPGISLKSYFPKNDDEAINITANVIKRLHKAPIPSTHAFPHIKDWLAALDGDIKFPVQ
TLQKAREIRDQLLKTAEPDVLLHGDLHHDNILQNCEDWLGIDPKGVIGEPAYEVAAFIRNPIPELLNHADAPNIIHNRIT
LFAELLELPPQRILDWCFVQVVLTWVWALEDGCDASYFEKITGIFDGMINPIKKSLQENS

Sequences:

>Translated_300_residues
MKTFEQNIINLYGEKGKQWLDNLPNLVTQLANTYELSNLKPVNNLSYNYVLSGFQGPQPIILKLGLDVNGIKCEAAALMS
FEDFGVVQVFSENTGLLLLECAVPGISLKSYFPKNDDEAINITANVIKRLHKAPIPSTHAFPHIKDWLAALDGDIKFPVQ
TLQKAREIRDQLLKTAEPDVLLHGDLHHDNILQNCEDWLGIDPKGVIGEPAYEVAAFIRNPIPELLNHADAPNIIHNRIT
LFAELLELPPQRILDWCFVQVVLTWVWALEDGCDASYFEKITGIFDGMINPIKKSLQENS
>Mature_300_residues
MKTFEQNIINLYGEKGKQWLDNLPNLVTQLANTYELSNLKPVNNLSYNYVLSGFQGPQPIILKLGLDVNGIKCEAAALMS
FEDFGVVQVFSENTGLLLLECAVPGISLKSYFPKNDDEAINITANVIKRLHKAPIPSTHAFPHIKDWLAALDGDIKFPVQ
TLQKAREIRDQLLKTAEPDVLLHGDLHHDNILQNCEDWLGIDPKGVIGEPAYEVAAFIRNPIPELLNHADAPNIIHNRIT
LFAELLELPPQRILDWCFVQVVLTWVWALEDGCDASYFEKITGIFDGMINPIKKSLQENS

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation [H]

COG id: COG3570

COG function: function code V; Streptomycin 6-kinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR006748 [H]

Pfam domain/function: PF04655 APH_6_hur [H]

EC number: =2.7.1.72 [H]

Molecular weight: Translated: 33676; Mature: 33676

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFEQNIINLYGEKGKQWLDNLPNLVTQLANTYELSNLKPVNNLSYNYVLSGFQGPQPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCCCE
ILKLGLDVNGIKCEAAALMSFEDFGVVQVFSENTGLLLLECAVPGISLKSYFPKNDDEAI
EEEECCCCCCCEEHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCHHHCCCCCCCCEE
NITANVIKRLHKAPIPSTHAFPHIKDWLAALDGDIKFPVQTLQKAREIRDQLLKTAEPDV
EHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
LLHGDLHHDNILQNCEDWLGIDPKGVIGEPAYEVAAFIRNPIPELLNHADAPNIIHNRIT
EEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHH
LFAELLELPPQRILDWCFVQVVLTWVWALEDGCDASYFEKITGIFDGMINPIKKSLQENS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTFEQNIINLYGEKGKQWLDNLPNLVTQLANTYELSNLKPVNNLSYNYVLSGFQGPQPI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCCCE
ILKLGLDVNGIKCEAAALMSFEDFGVVQVFSENTGLLLLECAVPGISLKSYFPKNDDEAI
EEEECCCCCCCEEHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCHHHCCCCCCCCEE
NITANVIKRLHKAPIPSTHAFPHIKDWLAALDGDIKFPVQTLQKAREIRDQLLKTAEPDV
EHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
LLHGDLHHDNILQNCEDWLGIDPKGVIGEPAYEVAAFIRNPIPELLNHADAPNIIHNRIT
EEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHH
LFAELLELPPQRILDWCFVQVVLTWVWALEDGCDASYFEKITGIFDGMINPIKKSLQENS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3118332; 3029728; 3039306 [H]