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The map label for this gene is dnaB [H]

Identifier: 189183213

GI number: 189183213

Start: 320851

End: 321282

Strand: Direct

Name: dnaB [H]

Synonym: OTT_0306

Alternate gene names: 189183213

Gene position: 320851-321282 (Clockwise)

Preceding gene: 189183212

Following gene: 189183214

Centisome position: 15.97

GC content: 31.94

Gene sequence:

>432_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCAGAGCAAATGATACTTGGAGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCAGAACATTTTTACGAGCCATTACATGGTAAAATATATAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAGGCAATGAACTAGCATTTGAG
GAAATAGGTGGAGTGAATTATCTAGCTAAACTTACAACTTTAGCATTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGCGGCGTTATTTTATTGAAATTGGAGAAAAAATAGTAACAAATGCATATTCTTCTACTTTAG
CAGATACAGCTATAAGTCAGATAGATGTATAG

Upstream 100 bases:

>100_bases
GAAACAACTGTATGCAAGGTCTTGAATATGCTTTTAAAGCTCAGGGATTTTCTTTTGAGTTACTTAAGTTTAGTAATTTT
AATAAAATTTAAAGAGTGAT

Downstream 100 bases:

>100_bases
TTTAAGAAAAATTAACTAACTAGAAAAAATAAGTCAAAGCAAGAGTTATTGTGAGCTTTATACGCCTTATAAAATCTGCT
ATGTAGTTTATTCTCGTTTG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVNYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADTAISQIDV

Sequences:

>Translated_143_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVNYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADTAISQIDV
>Mature_143_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLGNELAFE
EIGGVNYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEIGEKIVTNAYSSTLADTAISQIDV

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=141, Percent_Identity=26.9503546099291, Blast_Score=66, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 15893; Mature: 15893

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHHCCCEEECHHHHHCCHHHHCHHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVNYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADTAISQIDV
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHHCCCEEECHHHHHCCHHHHCHHHHHHHHHHHHHHHC
GISATVISLKNMLGNELAFEEIGGVNYLAKLTTLALSIVNVNEYGKIVYDLALRRYFIEI
CCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADTAISQIDV
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA