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The map label for this gene is pdhC [H]

Identifier: 189183202

GI number: 189183202

Start: 304277

End: 304579

Strand: Direct

Name: pdhC [H]

Synonym: OTT_0295

Alternate gene names: 189183202

Gene position: 304277-304579 (Clockwise)

Preceding gene: 189183201

Following gene: 189183203

Centisome position: 15.15

GC content: 30.36

Gene sequence:

>303_bases
ATGTGTGCTCGAGCTAATGAACTTAAACCTAGTGAGTTTCAAGGTAGTAGTTTTACTATTTCTAATCTTGGTATGTATGG
TATTAAGCAATTTAATGCAATAATTAATCTTCCTCAGAGTTGTATACTTAGTATTAGAGCTACTATTAAGATGCCAGTCG
TTGTTGATAATCAAATTACTGTTGCTAGAGTAATGGATATATCACTTTCATGTGATCACAGAGTAGTTGATGGTATAGTT
GGAGCAAAGTTCTTAAATATTTTTAGAGAAATCATAGAAAATCCAATGATAATGTTAGTATAA

Upstream 100 bases:

>100_bases
AACGATTTGCTGATCAAGATTGCTGCTATAGCTTTAGTTAGAGTACCTTGTATGATAAAAAATCTTTATTTGTAATTTTT
AATGAGGCAAAAGTTTGATT

Downstream 100 bases:

>100_bases
TTGTTAATTAATTTATTACGTAATGAATATTTTACGTTTACTTAAAAACAAGTGCAGTTATATTATATTTGTTAACTTAT
ATTTTTATTCTATATAGATT

Product: dihydrolipoamide acetyltransferase component

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 100; Mature: 100

Protein sequence:

>100_residues
MCARANELKPSEFQGSSFTISNLGMYGIKQFNAIINLPQSCILSIRATIKMPVVVDNQITVARVMDISLSCDHRVVDGIV
GAKFLNIFREIIENPMIMLV

Sequences:

>Translated_100_residues
MCARANELKPSEFQGSSFTISNLGMYGIKQFNAIINLPQSCILSIRATIKMPVVVDNQITVARVMDISLSCDHRVVDGIV
GAKFLNIFREIIENPMIMLV
>Mature_100_residues
MCARANELKPSEFQGSSFTISNLGMYGIKQFNAIINLPQSCILSIRATIKMPVVVDNQITVARVMDISLSCDHRVVDGIV
GAKFLNIFREIIENPMIMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=100, Percent_Identity=57, Blast_Score=118, Evalue=1e-27,
Organism=Homo sapiens, GI260898739, Length=99, Percent_Identity=40.4040404040404, Blast_Score=83, Evalue=6e-17,
Organism=Homo sapiens, GI203098753, Length=99, Percent_Identity=40.4040404040404, Blast_Score=82, Evalue=8e-17,
Organism=Homo sapiens, GI203098816, Length=99, Percent_Identity=40.4040404040404, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI19923748, Length=98, Percent_Identity=36.734693877551, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786946, Length=98, Percent_Identity=35.7142857142857, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1786305, Length=92, Percent_Identity=36.9565217391304, Blast_Score=65, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560088, Length=100, Percent_Identity=50, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538894, Length=98, Percent_Identity=42.8571428571429, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25146366, Length=98, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324258, Length=103, Percent_Identity=42.7184466019417, Blast_Score=77, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320352, Length=98, Percent_Identity=37.7551020408163, Blast_Score=73, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24582497, Length=101, Percent_Identity=46.5346534653465, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI20129315, Length=101, Percent_Identity=46.5346534653465, Blast_Score=98, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24645909, Length=97, Percent_Identity=35.0515463917526, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859875, Length=99, Percent_Identity=34.3434343434343, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 11101; Mature: 11101

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
9.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCARANELKPSEFQGSSFTISNLGMYGIKQFNAIINLPQSCILSIRATIKMPVVVDNQIT
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHEECCEEECCCEE
VARVMDISLSCDHRVVDGIVGAKFLNIFREIIENPMIMLV
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MCARANELKPSEFQGSSFTISNLGMYGIKQFNAIINLPQSCILSIRATIKMPVVVDNQIT
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHEECCEEECCCEE
VARVMDISLSCDHRVVDGIVGAKFLNIFREIIENPMIMLV
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]