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The map label for this gene is dnaG [H]

Identifier: 189183186

GI number: 189183186

Start: 280344

End: 282119

Strand: Direct

Name: dnaG [H]

Synonym: OTT_0279

Alternate gene names: 189183186

Gene position: 280344-282119 (Clockwise)

Preceding gene: 189183185

Following gene: 189183187

Centisome position: 13.95

GC content: 30.12

Gene sequence:

>1776_bases
ATGAGATCTACTAATTTCTTTTATGAAAATATTAGAACTATACTCAATGTTTCTACGATTGTTAAGCAAAAAGTTACTCT
TATTAAAAGAGGAGGAGAATATGTAGGAATTTGTCCATTTCATTCTGAGAAAACACCATCATTTACTGTTAACGACCGCA
AGAAATTTTATCATTGTTTTGGCTGCTCTGCTCATGGTGATGTGATAAAATTTGTGGCAGATACTTCTGGTCTATCTTAT
AGAGAATCAGCGCTAAGGCTAGCTCAAGAAAATGGTATTGATATACCAGTATTTTCAAAAGAGCAAGAAAAATTATATGA
AGAAATAGATATAATTCATAATACACTTGAGCTTGCACAGAATTTTTTCATTGCTTCTCTTTCTAATGATATTAGGTCAT
ATTTATATGACAGAGGATTATCTGATCTCCAAATCGAAAAATATAAGATAGGTTATGCTCCAGGTAAAAATCTATTGCAG
GAATTTTTAGAAATGAGAGGAATTCCTTTAGCATTCATCGTAAAATCTGGATTAATAGCCAAAAATGATAATGGGCAAAG
CTATGAAGTTTTTAGGGATCGTATTATGTTTCCCATTATTAATGTAGCCAATAAAACCATAGGATTTGGAGGCAGAGTTA
TAAATAATAATAGCCAACCAAAATACCTTAACTCTCCAGAAACTGCTGTATTTAAGAAAAATGAAGCATTATATGGTGAA
GATATTGCAACTAGTCATATTTACTCTAAAAATAGAGCGATAGTTGTAGAAGGATATATGGACTTAATTGCTTTGCAATC
TCAAGGTATTTATGAAACAGTAGCGACTTTAGGAACAGCTCTAACTGAAAAACATTTACACAGATTATGGCAAATTTGCG
ATGAAATAATTTTATGCTTTGATGGAGATCAAGCTGGCAACAGAGCTATGCAGAGGGCTGTTATAAAAGTTTTACCACAT
ATTAGTTGTTCTCAAAGTATGAGTTTTATCATGTTACCAGATGGATTAGATCCAGATCAACTATTAAAACAGCATGGAAT
TACGCAGTTTCAAAAACTTATAGATACAAGGCTAGCAGTTTCAGAAACTTTATGGCGTTTAGAAACAAATGGTAAAGTAT
TTTCTACAGCAGAATCTAAAGCTCAGTTAGAGAAAAAAATTAATAATTATATTAAAGAAATCAAAGATTGGATATTAATA
AAAAACTATCAAACATATTTTAAACAAAAATATTGGCAATTAAATAGGTTTAATAACAATAACTCATATTTTGACAAACA
AAAATTAAATCTGAACATTCCAACTAGCAAAGAATACCTAGAGTACCTGTTATTATCACTAATAGCACACTTTCCAGAGT
TGCTAAACGATGATGTAATTGCAGAAAATTTTGTTAATCTTGAATTTTGTAATAAAGAAGCATATAATGCTAGAAAATGG
TTGATAGATAGCATATCAGGTAATAACTTCAGTCTACTGGATTCGATAAAAACTGACATTACTGAATCTATACTTAAATT
ACCTGAAACAGTGTGTCGTATGTCGCTAACAATAAATAAAAAATACGGCGCAAAGTTATTATGGAGTTTATTTTATAAAA
AGTATCAAGTTCTGCTTATAAAAGAAGAATATGTTAATAGTTTAACCATAGATTGTCAAAATAGTGTTGACAATGCGAGT
CTATATCAGCAAGAAATTTTTAAAATTGAACATGAAATTGACCTTTTAAATGAAGCTTTACACTTAGAACAAGAAGAACA
TGGCAAGTCTGCGTAA

Upstream 100 bases:

>100_bases
TCAACAATACCTAAAAAATTGCACTTGTTTTATAAGGCAATAGAAAATATGATAGTTTGTTATGTAAAGTTAACAACAAT
CACTTTAATTGGTTATACTA

Downstream 100 bases:

>100_bases
TAAAAATAATAAAATTATTATCTGCAATAAAAAAACTAAAAAAAATACAAATGTAGAAGAGTTAGCTAGCAATTTCGTTG
ATGAATCAGAAAAATTATCA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRSTNFFYENIRTILNVSTIVKQKVTLIKRGGEYVGICPFHSEKTPSFTVNDRKKFYHCFGCSAHGDVIKFVADTSGLSY
RESALRLAQENGIDIPVFSKEQEKLYEEIDIIHNTLELAQNFFIASLSNDIRSYLYDRGLSDLQIEKYKIGYAPGKNLLQ
EFLEMRGIPLAFIVKSGLIAKNDNGQSYEVFRDRIMFPIINVANKTIGFGGRVINNNSQPKYLNSPETAVFKKNEALYGE
DIATSHIYSKNRAIVVEGYMDLIALQSQGIYETVATLGTALTEKHLHRLWQICDEIILCFDGDQAGNRAMQRAVIKVLPH
ISCSQSMSFIMLPDGLDPDQLLKQHGITQFQKLIDTRLAVSETLWRLETNGKVFSTAESKAQLEKKINNYIKEIKDWILI
KNYQTYFKQKYWQLNRFNNNNSYFDKQKLNLNIPTSKEYLEYLLLSLIAHFPELLNDDVIAENFVNLEFCNKEAYNARKW
LIDSISGNNFSLLDSIKTDITESILKLPETVCRMSLTINKKYGAKLLWSLFYKKYQVLLIKEEYVNSLTIDCQNSVDNAS
LYQQEIFKIEHEIDLLNEALHLEQEEHGKSA

Sequences:

>Translated_591_residues
MRSTNFFYENIRTILNVSTIVKQKVTLIKRGGEYVGICPFHSEKTPSFTVNDRKKFYHCFGCSAHGDVIKFVADTSGLSY
RESALRLAQENGIDIPVFSKEQEKLYEEIDIIHNTLELAQNFFIASLSNDIRSYLYDRGLSDLQIEKYKIGYAPGKNLLQ
EFLEMRGIPLAFIVKSGLIAKNDNGQSYEVFRDRIMFPIINVANKTIGFGGRVINNNSQPKYLNSPETAVFKKNEALYGE
DIATSHIYSKNRAIVVEGYMDLIALQSQGIYETVATLGTALTEKHLHRLWQICDEIILCFDGDQAGNRAMQRAVIKVLPH
ISCSQSMSFIMLPDGLDPDQLLKQHGITQFQKLIDTRLAVSETLWRLETNGKVFSTAESKAQLEKKINNYIKEIKDWILI
KNYQTYFKQKYWQLNRFNNNNSYFDKQKLNLNIPTSKEYLEYLLLSLIAHFPELLNDDVIAENFVNLEFCNKEAYNARKW
LIDSISGNNFSLLDSIKTDITESILKLPETVCRMSLTINKKYGAKLLWSLFYKKYQVLLIKEEYVNSLTIDCQNSVDNAS
LYQQEIFKIEHEIDLLNEALHLEQEEHGKSA
>Mature_591_residues
MRSTNFFYENIRTILNVSTIVKQKVTLIKRGGEYVGICPFHSEKTPSFTVNDRKKFYHCFGCSAHGDVIKFVADTSGLSY
RESALRLAQENGIDIPVFSKEQEKLYEEIDIIHNTLELAQNFFIASLSNDIRSYLYDRGLSDLQIEKYKIGYAPGKNLLQ
EFLEMRGIPLAFIVKSGLIAKNDNGQSYEVFRDRIMFPIINVANKTIGFGGRVINNNSQPKYLNSPETAVFKKNEALYGE
DIATSHIYSKNRAIVVEGYMDLIALQSQGIYETVATLGTALTEKHLHRLWQICDEIILCFDGDQAGNRAMQRAVIKVLPH
ISCSQSMSFIMLPDGLDPDQLLKQHGITQFQKLIDTRLAVSETLWRLETNGKVFSTAESKAQLEKKINNYIKEIKDWILI
KNYQTYFKQKYWQLNRFNNNNSYFDKQKLNLNIPTSKEYLEYLLLSLIAHFPELLNDDVIAENFVNLEFCNKEAYNARKW
LIDSISGNNFSLLDSIKTDITESILKLPETVCRMSLTINKKYGAKLLWSLFYKKYQVLLIKEEYVNSLTIDCQNSVDNAS
LYQQEIFKIEHEIDLLNEALHLEQEEHGKSA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=475, Percent_Identity=33.2631578947368, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68039; Mature: 68039

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTNFFYENIRTILNVSTIVKQKVTLIKRGGEYVGICPFHSEKTPSFTVNDRKKFYHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCHHEEEEE
GCSAHGDVIKFVADTSGLSYRESALRLAQENGIDIPVFSKEQEKLYEEIDIIHNTLELAQ
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFFIASLSNDIRSYLYDRGLSDLQIEKYKIGYAPGKNLLQEFLEMRGIPLAFIVKSGLIA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHCCCEE
KNDNGQSYEVFRDRIMFPIINVANKTIGFGGRVINNNSQPKYLNSPETAVFKKNEALYGE
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCC
DIATSHIYSKNRAIVVEGYMDLIALQSQGIYETVATLGTALTEKHLHRLWQICDEIILCF
CHHHHHHCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
DGDQAGNRAMQRAVIKVLPHISCSQSMSFIMLPDGLDPDQLLKQHGITQFQKLIDTRLAV
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHH
SETLWRLETNGKVFSTAESKAQLEKKINNYIKEIKDWILIKNYQTYFKQKYWQLNRFNNN
HHHHHHEECCCCEEECHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCC
NSYFDKQKLNLNIPTSKEYLEYLLLSLIAHFPELLNDDVIAENFVNLEFCNKEAYNARKW
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCHHCCHHHH
LIDSISGNNFSLLDSIKTDITESILKLPETVCRMSLTINKKYGAKLLWSLFYKKYQVLLI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHEEEEE
KEEYVNSLTIDCQNSVDNASLYQQEIFKIEHEIDLLNEALHLEQEEHGKSA
EHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MRSTNFFYENIRTILNVSTIVKQKVTLIKRGGEYVGICPFHSEKTPSFTVNDRKKFYHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCHHEEEEE
GCSAHGDVIKFVADTSGLSYRESALRLAQENGIDIPVFSKEQEKLYEEIDIIHNTLELAQ
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFFIASLSNDIRSYLYDRGLSDLQIEKYKIGYAPGKNLLQEFLEMRGIPLAFIVKSGLIA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHCCCEE
KNDNGQSYEVFRDRIMFPIINVANKTIGFGGRVINNNSQPKYLNSPETAVFKKNEALYGE
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCC
DIATSHIYSKNRAIVVEGYMDLIALQSQGIYETVATLGTALTEKHLHRLWQICDEIILCF
CHHHHHHCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
DGDQAGNRAMQRAVIKVLPHISCSQSMSFIMLPDGLDPDQLLKQHGITQFQKLIDTRLAV
CCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCHHHHHHHHHHHHHH
SETLWRLETNGKVFSTAESKAQLEKKINNYIKEIKDWILIKNYQTYFKQKYWQLNRFNNN
HHHHHHEECCCCEEECHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCC
NSYFDKQKLNLNIPTSKEYLEYLLLSLIAHFPELLNDDVIAENFVNLEFCNKEAYNARKW
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEECCCHHCCHHHH
LIDSISGNNFSLLDSIKTDITESILKLPETVCRMSLTINKKYGAKLLWSLFYKKYQVLLI
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHEEEEE
KEEYVNSLTIDCQNSVDNASLYQQEIFKIEHEIDLLNEALHLEQEEHGKSA
EHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]