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The map label for this gene is traD [H]

Identifier: 189183108

GI number: 189183108

Start: 194602

End: 195183

Strand: Reverse

Name: traD [H]

Synonym: OTT_0201

Alternate gene names: 189183108

Gene position: 195183-194602 (Counterclockwise)

Preceding gene: 189183109

Following gene: 189183099

Centisome position: 9.72

GC content: 34.19

Gene sequence:

>582_bases
TTGTTTTCTTTACAGCTCAAGGTAAAAAAAACGATTATTAAAGCCAAAATTAGAGGTGCTGATTTTGTACGGAGTAGAAA
TCTAGCCAAAATGCTAAAAAGCGCTAAAAAGGCCTCGAAAATCTGTTTTGGAGACTTGCTATTAGTAAAGAATAGTGAAA
GGCTACATATTCTTATTACTGGAACAACAGGTACTGGTAAAACTAATATGCTTAATGAACTGCAACCACAGATTCGATTA
CACAAAGATCGAGCAATAATTGTAGATACAACTGGAGCTTTTACTGATAGATTTTTTGACCCTAAATGTGATAAACTGCT
TAATCCTTTGGAAAAAAATAGTGAACAATGGTTGCCTTGGAATGATTGTTTTGAAGCAAAAGCTCCAGTTGTGTCTACAA
TTATTGCTCGATCTTTGTGTAATCGAATTTGTGGTAGCAGTTCATTAAGCATATTAGTTTTACCAGTACCTGTTGTTCCA
GTAATAAGAATGTGCAGCCTTTTACTATTCTTTACTAATGGCAAGCCTCCAAAACAGATTTTTGAGGCCTTTTTAGCGCT
TTTTAGCATTTTAGATAGGTAA

Upstream 100 bases:

>100_bases
GGCCAACATGGATTTAAAATACAGCAATTATGGGAATTCCTAATCAATTCAGCATTATTAGAAGGATTAATTGTTTTTGC
TATTGGTGTGATAATCTCAA

Downstream 100 bases:

>100_bases
TTTATACTCCACAAAATCAGCGCCTCTAATTTTGGCCTTAATAATCGTGTTTTTGCCTTGAGCTGTAAAGAAAACAATTG
AAATTATTACACCAATAGTA

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MFSLQLKVKKTIIKAKIRGADFVRSRNLAKMLKSAKKASKICFGDLLLVKNSERLHILITGTTGTGKTNMLNELQPQIRL
HKDRAIIVDTTGAFTDRFFDPKCDKLLNPLEKNSEQWLPWNDCFEAKAPVVSTIIARSLCNRICGSSSLSILVLPVPVVP
VIRMCSLLLFFTNGKPPKQIFEAFLALFSILDR

Sequences:

>Translated_193_residues
MFSLQLKVKKTIIKAKIRGADFVRSRNLAKMLKSAKKASKICFGDLLLVKNSERLHILITGTTGTGKTNMLNELQPQIRL
HKDRAIIVDTTGAFTDRFFDPKCDKLLNPLEKNSEQWLPWNDCFEAKAPVVSTIIARSLCNRICGSSSLSILVLPVPVVP
VIRMCSLLLFFTNGKPPKQIFEAFLALFSILDR
>Mature_193_residues
MFSLQLKVKKTIIKAKIRGADFVRSRNLAKMLKSAKKASKICFGDLLLVKNSERLHILITGTTGTGKTNMLNELQPQIRL
HKDRAIIVDTTGAFTDRFFDPKCDKLLNPLEKNSEQWLPWNDCFEAKAPVVSTIIARSLCNRICGSSSLSILVLPVPVVP
VIRMCSLLLFFTNGKPPKQIFEAFLALFSILDR

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 21661; Mature: 21661

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLQLKVKKTIIKAKIRGADFVRSRNLAKMLKSAKKASKICFGDLLLVKNSERLHILIT
CCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEE
GTTGTGKTNMLNELQPQIRLHKDRAIIVDTTGAFTDRFFDPKCDKLLNPLEKNSEQWLPW
ECCCCCCHHHHHHHCHHHEEECCCEEEEECCCCHHHCCCCCCHHHHHCHHHCCCCCCCCH
NDCFEAKAPVVSTIIARSLCNRICGSSSLSILVLPVPVVPVIRMCSLLLFFTNGKPPKQI
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHH
FEAFLALFSILDR
HHHHHHHHHHHCC
>Mature Secondary Structure
MFSLQLKVKKTIIKAKIRGADFVRSRNLAKMLKSAKKASKICFGDLLLVKNSERLHILIT
CCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEE
GTTGTGKTNMLNELQPQIRLHKDRAIIVDTTGAFTDRFFDPKCDKLLNPLEKNSEQWLPW
ECCCCCCHHHHHHHCHHHEEECCCEEEEECCCCHHHCCCCCCHHHHHCHHHCCCCCCCCH
NDCFEAKAPVVSTIIARSLCNRICGSSSLSILVLPVPVVPVIRMCSLLLFFTNGKPPKQI
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHH
FEAFLALFSILDR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]