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The map label for this gene is traA [H]

Identifier: 189183096

GI number: 189183096

Start: 185050

End: 187659

Strand: Direct

Name: traA [H]

Synonym: OTT_0189

Alternate gene names: 189183096

Gene position: 185050-187659 (Clockwise)

Preceding gene: 189183095

Following gene: 189183097

Centisome position: 9.21

GC content: 33.33

Gene sequence:

>2610_bases
ATGAATGAGGTAGAACGAACAGCAAAAAAAGACAACAGCCAGTTGTTGAAGGATATAGTAATAGCATTGCCAGACGAGAA
GGAGCTAAATTTAGAGCATAGAATAGAACTAACTCATCAAATAGTTGATGAAATGGAATGGGTGCAAAAAGGTATTGGAG
TACAGATAGACATTCATAAGCCTCAAACAGGAGATAAAAACTGGCATGTACATATATTGCTTACTATGAGAAGATTTAGA
GAAGATGGAACTGGTTTAGGAGATATAGCAGTAGATTTAAACCCAAAAATCATAACATTAAGTAATGGCAAAAAGGTTGT
TATTAAAGATCCCGAGATGATTCATGAAAGAGTAAAAGAAATAATTAATGCATTTTTCGCTAAATTAGGCTTACCATATA
GAGTTAAGGATACCAGTAAAGTGCCGGGAAAGCATATTGGACCTCGTAGAATTAGGAATTTAATTAATGAAGTATTAAAT
GAAAATGAGTTACGTAAAGAGGCTCATTTGAAGATTATTAATGATGCTGACGTAATAACAGATTCTATAACACATTACAA
ATCTATTTTTACTAAGCAGGATGTTGAAAAAGCAGTAAAAGATATACCAGATCCAACAGCAAGAGAACAGTTAGTTCAGC
AAGTGCTTAGTTCAAATAGAATACTAGAGTTATACCATGATGATGGTGAAAGTAGCAAATATTTTACGACAACTGAGGTT
CGAAATGAGGAGACGAGAATAATCAGAATAGCTAATAAAATCAATGATCAGGTTTATTACAACAACATTTACAATCTTAA
AAGTGATATAGAAGGTCTAGCAAATGTTAGTGAGGAACAGAAACAAGCTCTAAGGCATATTTTGCTTAGCACTAGTGGAG
TTAGAGTCTTGAGAGGAAGAGCTGGTACAGGAAAATCTTATGTTTTAATAAAAGCGCATAAGCTCGCAACAAATCGTAGA
CAAAAAGTTATTGGTCTTGCTCCTACTCATAAGGCAGTATCAGAGCTGAGGAGCAAAGGTTATACAGAGGTCTATACAGT
AAAAGGATTTTTGTATAATCGAAAAAAAAATTTTATGCAAGGCAGCTTAATAGTAGTAGATGAAGCTGGAATGGTAGGTA
CTAAAGCTTATGCAGAGCTGTTTAGAGTAGTTAGAAACAATTATTGTCAACTGATACTCGCTGGAGATGAAAAACAGCTA
GCTTCAATAGAAAGAGGCGGAATGTTTGAGATGCTGAGTAATAATTTCGGTTCACATGTTTTAATAGATATTCGCAGACA
AAGTGAAAACTGGAGCAGAGAAGCAGCAACAAAGTTTGCTGAGAGTAATATTTTAAGCGGTATAACCTTACTGAGACAAA
ATAAATGTGTTAAGTTTGATAATACATTACAGGATTCAATTAGTAAGTTGATTTATGATTGGAGTCTAAGCAAGTTTAAG
CTACATGAAAAATTGGTAATTACAGTACGTAATAAAGATGTCGATATTCTTAATTCAAGTATTAGATCTTTGTTAAAAGC
TAATGGCACGCTACAAGGCACAGAATATAGGCGTTCAATTGCTGGAAGGAAAGAGTCCTATATGGCAGGAGATAGAATCG
TATTTCAAAAAAGCGATAAGGATTTACAAATACAAAACAGTGAATTTGCAACTTTAACTTCTGTTAATAAAAATGAATTT
GTAGCTAAAACAGATGCAGGAAAAGAGGTGAGTTTTGATCCTAGTAAAATACAATTTAAACATGGCTATGCAAGTACTAT
TTATAAGGCTCAGGGAGCTTCTATAAAAGATGTATACGTTTTGCATAATGGAGTAAGTAATATAAGCAGCTCATATGTAG
CTATGACAAGGCATATAGAGAAGTTGCAGCTATATTGCAATAAGAAAGCTACTGTAAGCATTAAAAGTTTAATAAATCAG
CTCAGCAGACCAAATGAGAAATCAGCTAGCATAACTCTAAAAACTGCTCATGATTTGGAGAAAGAACGGACAAAGACAAC
TGTTTTTAGTAAAGTTTTTAGTAAAGTTGAAAACTGGTTTAAGTCTATAATCAATGATATCAATGATAGATCTCATGTGA
ATGAAGAATATTATCATTTTACCGCTAAACCAGAGGAAGAAGCTAAAGTAGAAAAAGTCCAGCTCAAAGATATTTCTACT
CCATTATTTATGCACATCAAAGAACAAAGACAGTATGATTATGATGTAACCATTCTGTCAGCAGAAGGAAAAACGATATC
AAGTTTTCAGGAAGCTGGCATTGATAGCAGAATGGTATATAGCTCAAATGTTAATAATTTGAAGTACTATCAACCATTTC
AAGGAGAAAAGATTCTTATAGCTGCAAATAATGATAAACAGAATAAAGAGTATGCAAGTACTATAAACGAGGCTGCAAAA
GCATTAAAAAGCAAAGGAGCAATCACTAGCATCGTAGTTCCTTCAGAAGGTGAAGATTTTAACGAAATGCTAAAAAATAA
AGGAGCCACAGCTGTTAAGGAGCTTATGATACCTGAAATCATGAAGTTAATTAATACTCAGAACGTAAAAACAGAGTCTG
AACAAGTGGTGAAAACTGATATAGCTCAAAAAATTGTAATTAGGAGATAG

Upstream 100 bases:

>100_bases
AGGTATAAAGTATAACTTCTCTCGTAAGAAAGATAACGTATATCATACAGTGCTGATACCGGATTATGTAAAACAAGAAT
TCAAGAATATTCAAACATTA

Downstream 100 bases:

>100_bases
TTCTTACGATTAAAAATTGAATGTAAGCTATTCATTCACAGAGCGAATTACAAAATGAGCTATACATAACAGCTCACAGT
GATGCTTGCAATTCGCATTA

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 869; Mature: 869

Protein sequence:

>869_residues
MNEVERTAKKDNSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHKPQTGDKNWHVHILLTMRRFR
EDGTGLGDIAVDLNPKIITLSNGKKVVIKDPEMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLN
ENELRKEAHLKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDPTAREQLVQQVLSSNRILELYHDDGESSKYFTTTEV
RNEETRIIRIANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRR
QKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNYCQLILAGDEKQL
ASIERGGMFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSGITLLRQNKCVKFDNTLQDSISKLIYDWSLSKFK
LHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESYMAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEF
VAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQ
LSRPNEKSASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDINDRSHVNEEYYHFTAKPEEEAKVEKVQLKDIST
PLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYASTINEAAK
ALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEIMKLINTQNVKTESEQVVKTDIAQKIVIRR

Sequences:

>Translated_869_residues
MNEVERTAKKDNSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHKPQTGDKNWHVHILLTMRRFR
EDGTGLGDIAVDLNPKIITLSNGKKVVIKDPEMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLN
ENELRKEAHLKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDPTAREQLVQQVLSSNRILELYHDDGESSKYFTTTEV
RNEETRIIRIANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRR
QKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNYCQLILAGDEKQL
ASIERGGMFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSGITLLRQNKCVKFDNTLQDSISKLIYDWSLSKFK
LHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESYMAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEF
VAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQ
LSRPNEKSASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDINDRSHVNEEYYHFTAKPEEEAKVEKVQLKDIST
PLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYASTINEAAK
ALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEIMKLINTQNVKTESEQVVKTDIAQKIVIRR
>Mature_869_residues
MNEVERTAKKDNSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHKPQTGDKNWHVHILLTMRRFR
EDGTGLGDIAVDLNPKIITLSNGKKVVIKDPEMIHERVKEIINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLN
ENELRKEAHLKIINDADVITDSITHYKSIFTKQDVEKAVKDIPDPTAREQLVQQVLSSNRILELYHDDGESSKYFTTTEV
RNEETRIIRIANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRR
QKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNYCQLILAGDEKQL
ASIERGGMFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSGITLLRQNKCVKFDNTLQDSISKLIYDWSLSKFK
LHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESYMAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEF
VAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQ
LSRPNEKSASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDINDRSHVNEEYYHFTAKPEEEAKVEKVQLKDIST
PLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYASTINEAAK
ALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEIMKLINTQNVKTESEQVVKTDIAQKIVIRR

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 98882; Mature: 98882

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVERTAKKDNSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHK
CCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
PQTGDKNWHVHILLTMRRFREDGTGLGDIAVDLNPKIITLSNGKKVVIKDPEMIHERVKE
CCCCCCCEEEEEEEEHHHHHHCCCCCCEEEEECCCEEEEECCCCEEEEECHHHHHHHHHH
IINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLNENELRKEAHLKIINDADVIT
HHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCEEEECCHHHHH
DSITHYKSIFTKQDVEKAVKDIPDPTAREQLVQQVLSSNRILELYHDDGESSKYFTTTEV
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEC
RNEETRIIRIANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGR
CCCCHHEEEEHHHHCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECC
AGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQ
CCCCCEEEEEEEHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCC
GSLIVVDEAGMVGTKAYAELFRVVRNNYCQLILAGDEKQLASIERGGMFEMLSNNFGSHV
CCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHHHHCCCCCEE
LIDIRRQSENWSREAATKFAESNILSGITLLRQNKCVKFDNTLQDSISKLIYDWSLSKFK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEE
LHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESYMAGDRIVFQKSDK
ECEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCCCEEEEEECCC
DLQIQNSEFATLTSVNKNEFVAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYV
CEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHEECCCCCCEEEEE
LHNGVSNISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQLSRPNEKSASITLKTAHDLE
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCEEEEEEHHHHH
KERTKTTVFSKVFSKVENWFKSIINDINDRSHVNEEYYHFTAKPEEEAKVEKVQLKDIST
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHEEEECCCCHHHHHHHHHHHHCCC
PLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNLKYYQPFQGEKILI
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCEEECCCCCCEEEE
AANNDKQNKEYASTINEAAKALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEI
EECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHH
MKLINTQNVKTESEQVVKTDIAQKIVIRR
HHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEVERTAKKDNSQLLKDIVIALPDEKELNLEHRIELTHQIVDEMEWVQKGIGVQIDIHK
CCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
PQTGDKNWHVHILLTMRRFREDGTGLGDIAVDLNPKIITLSNGKKVVIKDPEMIHERVKE
CCCCCCCEEEEEEEEHHHHHHCCCCCCEEEEECCCEEEEECCCCEEEEECHHHHHHHHHH
IINAFFAKLGLPYRVKDTSKVPGKHIGPRRIRNLINEVLNENELRKEAHLKIINDADVIT
HHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCEEEECCHHHHH
DSITHYKSIFTKQDVEKAVKDIPDPTAREQLVQQVLSSNRILELYHDDGESSKYFTTTEV
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEC
RNEETRIIRIANKINDQVYYNNIYNLKSDIEGLANVSEEQKQALRHILLSTSGVRVLRGR
CCCCHHEEEEHHHHCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECC
AGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQ
CCCCCEEEEEEEHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCC
GSLIVVDEAGMVGTKAYAELFRVVRNNYCQLILAGDEKQLASIERGGMFEMLSNNFGSHV
CCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHHHHCCCCCEE
LIDIRRQSENWSREAATKFAESNILSGITLLRQNKCVKFDNTLQDSISKLIYDWSLSKFK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEE
LHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESYMAGDRIVFQKSDK
ECEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCCCEEEEEECCC
DLQIQNSEFATLTSVNKNEFVAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYV
CEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHEECCCCCCEEEEE
LHNGVSNISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQLSRPNEKSASITLKTAHDLE
EECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCEEEEEEHHHHH
KERTKTTVFSKVFSKVENWFKSIINDINDRSHVNEEYYHFTAKPEEEAKVEKVQLKDIST
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHEEEECCCCHHHHHHHHHHHHCCC
PLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNLKYYQPFQGEKILI
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCEEECCCCCCEEEE
AANNDKQNKEYASTINEAAKALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEI
EECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHH
MKLINTQNVKTESEQVVKTDIAQKIVIRR
HHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763953; 11743193; 11743194 [H]