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The map label for this gene is traA [H]

Identifier: 189183079

GI number: 189183079

Start: 168769

End: 170226

Strand: Direct

Name: traA [H]

Synonym: OTT_0172

Alternate gene names: 189183079

Gene position: 168769-170226 (Clockwise)

Preceding gene: 189183078

Following gene: 189183080

Centisome position: 8.4

GC content: 32.65

Gene sequence:

>1458_bases
ATGATTCATGAAATAGCAAAAGAAATAATTAATGCATATTTCGCTAAATTAGGCTTACCATATAGAGTTGACGAGAAAAG
CAAAGTGCCGGGAAAGCATATTGGGAATATTAAATATATTGAAATTAGGAATTTAATTAATGAAGTAGTAAATGAAAATG
AGTTACGTAAAGAGGCTCATTTGAAGATTATTAATGATGCTGACGTAATAACAGATTCTATAACACATTACAAATCTATT
TTTACTAAGCAGGATGTTGAAAAAGCCGTTCAAGATATACCAGATCCAACAGCAAGAAAACAGTTAGTTCAGCAAGTGCT
TAGTTCAAATAGAATACTAGAGTTATATCATGATGATGGTGAAAGTAGCAAATATTTTACGACAATTGAGGTTCGAAATG
AGGAGACGAGAATAATCAGAATAGCTAATAAAATCAATAATCAGGTTTATTACAACGATATTTACAATCTTAAAAGTGAT
ATCGAAGGTCTAGCAAATGTTAGTGAAGAACAGAAACAAGCTCTAAGGCATATTTTGCTTAGCACTAGTGGAGTTAGAGT
CTTGAGAGGAAGAGCTGGTACAGGAAAATCTTATGTTTTAATAAAAGCGCATAAGCTCGCAACAAATCGTAGACAAAAAG
TTATTGGTCTTGCTCCTACTCATAAGGCAGTATCAGAGCTGAGGAGCAAAGGTTATACAGAGGTCTATACAGTAAAAGGA
TTTTTGTATAATCGAAAAAAAAATTTTATGCAAGGCAGCTTAATAGTAGTAGATGAAGCTGGAATGGTAGGTACTAAAGC
TTATGCAGAGCTGTTTAGAGTAGTTAGAAACAATAATTGTCAACTGATACTTGCTGGAGATGAAAAGCAGTTAGCTTCAA
TAGAAAGAGGTGGAATGTTTGAGATGCTGAGTAATATTTTTGGTTCACATGTTTTAGTAAATATTCGAAGACAAAGTAAA
AACTGGAGCAGAGAAGCAGCAACAAAGTTTGCTGAGAGTAATATTTTAAGCTGTATAACCTTACTGAGGCAAAATAAATG
CGTTAGGTTTGATAATACGTTGCAGGACTCAATGAGTAAATTAATATACAACTGGAGTCTAAGCAAATTTAAACCACATG
AAAAATTGGTAATTACAGTACGTAATAAAGATGTCGACATTCTTAATTCAAGTATTAGGTCTTTGTTAAAAGCAAATGGT
ACGCTAAAAGGCACAGAATATGAGCGTTCTATTGATGGAAGGAAAAAGTCATATATGGCAGGAGATAGAATCGTATTTCA
AAAAAGCTATAAGGATTTACAAATACAAAACAGTGAATTTGCAACTTTAACTTCGGTTAGTAAAAATAAATTTATAGCTA
AGACAGACACAGGAAAAGAAGTGAGTTTTGATTCGGTAAAATACAATTTAAACATGGCTATGCAAGTACTGTTTATAAGG
TCCAGGGAGCTTCTATAA

Upstream 100 bases:

>100_bases
ATTTAAAGAGAATGGTGAAGAATTGTGTAGTAAAGCTGTTAACTTAGAGCCTAAATTTAGAACGGTAAAAGGTCAGCCGT
ATATTATTTGAGATTCCGAG

Downstream 100 bases:

>100_bases
AAGATGTATATGTTTTGCATAATGGAGTAAGTAATATAAGCAGCTCATACGTAGCTATGACAAGGTATATAGAGAACTTA
AAGTTATATTGCAATAAGGA

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MIHEIAKEIINAYFAKLGLPYRVDEKSKVPGKHIGNIKYIEIRNLINEVVNENELRKEAHLKIINDADVITDSITHYKSI
FTKQDVEKAVQDIPDPTARKQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIRIANKINNQVYYNDIYNLKSD
IEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKG
FLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSK
NWSREAATKFAESNILSCITLLRQNKCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANG
TLKGTEYERSIDGRKKSYMAGDRIVFQKSYKDLQIQNSEFATLTSVSKNKFIAKTDTGKEVSFDSVKYNLNMAMQVLFIR
SRELL

Sequences:

>Translated_485_residues
MIHEIAKEIINAYFAKLGLPYRVDEKSKVPGKHIGNIKYIEIRNLINEVVNENELRKEAHLKIINDADVITDSITHYKSI
FTKQDVEKAVQDIPDPTARKQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIRIANKINNQVYYNDIYNLKSD
IEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKG
FLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSK
NWSREAATKFAESNILSCITLLRQNKCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANG
TLKGTEYERSIDGRKKSYMAGDRIVFQKSYKDLQIQNSEFATLTSVSKNKFIAKTDTGKEVSFDSVKYNLNMAMQVLFIR
SRELL
>Mature_485_residues
MIHEIAKEIINAYFAKLGLPYRVDEKSKVPGKHIGNIKYIEIRNLINEVVNENELRKEAHLKIINDADVITDSITHYKSI
FTKQDVEKAVQDIPDPTARKQLVQQVLSSNRILELYHDDGESSKYFTTIEVRNEETRIIRIANKINNQVYYNDIYNLKSD
IEGLANVSEEQKQALRHILLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKG
FLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSK
NWSREAATKFAESNILSCITLLRQNKCVRFDNTLQDSMSKLIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANG
TLKGTEYERSIDGRKKSYMAGDRIVFQKSYKDLQIQNSEFATLTSVSKNKFIAKTDTGKEVSFDSVKYNLNMAMQVLFIR
SRELL

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 55417; Mature: 55417

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHEIAKEIINAYFAKLGLPYRVDEKSKVPGKHIGNIKYIEIRNLINEVVNENELRKEAH
CHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCHHHHHHHHC
LKIINDADVITDSITHYKSIFTKQDVEKAVQDIPDPTARKQLVQQVLSSNRILELYHDDG
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCC
ESSKYFTTIEVRNEETRIIRIANKINNQVYYNDIYNLKSDIEGLANVSEEQKQALRHILL
CCCEEEEEEEEECCCCEEEEEEHHHCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
STSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKG
CCCCCEEEECCCCCCCEEEEEEEHHHHCCCHHEEEEECCCHHHHHHHHHCCCCEEEEEEH
FLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMF
HHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCHH
EMLSNIFGSHVLVNIRRQSKNWSREAATKFAESNILSCITLLRQNKCVRFDNTLQDSMSK
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
LIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLKGTEYERSIDGRKKSYMA
HHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHCC
GDRIVFQKSYKDLQIQNSEFATLTSVSKNKFIAKTDTGKEVSFDSVKYNLNMAMQVLFIR
CCEEEEEECCCCCEECCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHH
SRELL
HHCCC
>Mature Secondary Structure
MIHEIAKEIINAYFAKLGLPYRVDEKSKVPGKHIGNIKYIEIRNLINEVVNENELRKEAH
CHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCHHHHHHHHC
LKIINDADVITDSITHYKSIFTKQDVEKAVQDIPDPTARKQLVQQVLSSNRILELYHDDG
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCC
ESSKYFTTIEVRNEETRIIRIANKINNQVYYNDIYNLKSDIEGLANVSEEQKQALRHILL
CCCEEEEEEEEECCCCEEEEEEHHHCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
STSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKG
CCCCCEEEECCCCCCCEEEEEEEHHHHCCCHHEEEEECCCHHHHHHHHHCCCCEEEEEEH
FLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGGMF
HHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCHH
EMLSNIFGSHVLVNIRRQSKNWSREAATKFAESNILSCITLLRQNKCVRFDNTLQDSMSK
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
LIYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLKANGTLKGTEYERSIDGRKKSYMA
HHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHCC
GDRIVFQKSYKDLQIQNSEFATLTSVSKNKFIAKTDTGKEVSFDSVKYNLNMAMQVLFIR
CCEEEEEECCCCCEECCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHH
SRELL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763954 [H]