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The map label for this gene is dnaB [H]

Identifier: 189183073

GI number: 189183073

Start: 160184

End: 161632

Strand: Reverse

Name: dnaB [H]

Synonym: OTT_0166

Alternate gene names: 189183073

Gene position: 161632-160184 (Counterclockwise)

Preceding gene: 189183074

Following gene: 189183072

Centisome position: 8.05

GC content: 32.02

Gene sequence:

>1449_bases
ATGGAAAAAGATCTTGCAAAGATTGCTCCAAGTAATATTCAGGCAGAGCAAATGATACTTGGGGCAATTCTGATTAACAA
TCGTGCGCTATATAACATTAACGAATTTTTACTGCCGGAACATTTTTATGAACCATTACATGGCAAAATATACAAGTCAA
TTAATCTCATTATTAGTAAAGGAATTAGTGCTACTGTAATTTCGCTCAAAAATATGCTAAGCAATGAACCCGCATTTGAT
GAAATAGGTGGAGTGGATTATCTAGCTAAACTTACAACTTTAGCGTTAAGTATAGTTAATGTTAATGAGTACGGCAAAAT
AGTATATGATCTTGCGCTGAGGCGTTATTTAATTGAAATTGGAGAAAAAATAGTAACAAATGCATATTCTTCTACTTTAG
CAGATTTAGCTATAAGTCAGATCGAAACTGCTGAATCTCAATTATATGATCTAGCTTCAAGAGGAACTTTAAGTCAAGGA
TTTATAAAATTACAAACTTCAATTGAAGAATCATGGACATCAATTTCATCTGCTATTAAAAATAAAAACTCTATTAATGG
TATTAGTAGTGGACTACTTGACCTTGATTCAAAGCTTGGAGGATTTAAAAATTCTGACCTAATAATATTAGCTGGCAGGC
CTTCAATGGGTAAAACTGCTTTAGGAGTTAACTTAGCTGTAAATGCTTGTAAATATTTTTTATCATCATCTACTCAACAA
AATAGCAAAGTTTCTAATATAAAACCATCAGTTGGATTCTTTTCTTTAGAAATGTCATCTCAGCAAATCTCTACTCGAAT
TCTTTCTATAGAATCTGAAATTAATAGCTCTGCATTATTTAACGGTAAAATAGGTGAACAAGATGTTGATAAGTTAAAGA
CTGTACAAGATGAAATACAAAAGTGGAATTTTTTTATAGATGATGCTCCTGCAATCTCGATATCTGCAATTAGATCTCGA
GCTCGTAGACTTAAGCGTACTCATAATTTAGCAATATTATTTATTGATTATTTACAGCTAATAAAAATTGATTCCAGAGG
AAGTCAGTATAATAGAGTGCAGGAGATTTCTGAAATTACTCAGAGCTTAAAAGCTCTTGCTAAAGAGCTCAATATTCCAG
TCATTGCATTATCTCAATTGTCTAGAGCTGTAGAACAAAGGTCAGATAAAAAGCCTATTCTCTCAGATCTGAGAGAATCA
GGCTCAATTGAACAAGATGCCGATATTGTAATGCTTATATATAGAGAAGAGTATTATTTATCTAGATCAGAACCGCATCC
AGATTCTATGGAATACGAAGAATGGTTCATCAAACAAAATAAATGTTATAACACTGCTGAAATAATTGTTGCTAAACACC
GTAATGGGCCAGTTGGTACAGTAAACTTACACTATGATAATAGGTATTCTAAATTTGGCAATATTGTTAAAAACTCTCAG
CAAGGTTAA

Upstream 100 bases:

>100_bases
GAGAGAACTATCTGAAAGATCTTGAGCACTCTTTTAAAGCTCAAGGATTTTCTTTTGAGTTAGTTAAGTTTAGTAATTTT
AATAAAATTTAAAGGGTGAT

Downstream 100 bases:

>100_bases
TAAACACTAGATTATGCATAAATGAAAAAATGCTGGTCCAAAAAAAAATTACAGCAAAATTTTACTCATTAACACCAGAG
ATATGCAACTCAAGATTGGT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLSNEPAFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLASRGTLSQG
FIKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAVNACKYFLSSSTQQ
NSKVSNIKPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRES
GSIEQDADIVMLIYREEYYLSRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQ
QG

Sequences:

>Translated_482_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLSNEPAFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLASRGTLSQG
FIKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAVNACKYFLSSSTQQ
NSKVSNIKPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRES
GSIEQDADIVMLIYREEYYLSRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQ
QG
>Mature_482_residues
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISKGISATVISLKNMLSNEPAFD
EIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEIGEKIVTNAYSSTLADLAISQIETAESQLYDLASRGTLSQG
FIKLQTSIEESWTSISSAIKNKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAVNACKYFLSSSTQQ
NSKVSNIKPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQKWNFFIDDAPAISISAIRSR
ARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEITQSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRES
GSIEQDADIVMLIYREEYYLSRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQ
QG

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=475, Percent_Identity=36, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53811; Mature: 53811

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLSNEPAFDEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLASRGTLSQGFIKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAVNACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
NSKVSNIKPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCCCHHHHHHHHHHHH
QSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRESGSIEQDADIVMLIYREEYYL
HHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECHHHC
SRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQ
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHCCC
QG
CC
>Mature Secondary Structure
MEKDLAKIAPSNIQAEQMILGAILINNRALYNINEFLLPEHFYEPLHGKIYKSINLIISK
CCCCHHHHCCCCCCHHHHHHHHHHCCCCEEECCHHHHCCHHHHCCHHHHHHHHHHHHHHC
GISATVISLKNMLSNEPAFDEIGGVDYLAKLTTLALSIVNVNEYGKIVYDLALRRYLIEI
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHH
GEKIVTNAYSSTLADLAISQIETAESQLYDLASRGTLSQGFIKLQTSIEESWTSISSAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEHHHHHHHHHHHHHHHH
NKNSINGISSGLLDLDSKLGGFKNSDLIILAGRPSMGKTALGVNLAVNACKYFLSSSTQQ
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
NSKVSNIKPSVGFFSLEMSSQQISTRILSIESEINSSALFNGKIGEQDVDKLKTVQDEIQ
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
KWNFFIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSRGSQYNRVQEISEIT
HCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCCCHHHHHHHHHHHH
QSLKALAKELNIPVIALSQLSRAVEQRSDKKPILSDLRESGSIEQDADIVMLIYREEYYL
HHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECHHHC
SRSEPHPDSMEYEEWFIKQNKCYNTAEIIVAKHRNGPVGTVNLHYDNRYSKFGNIVKNSQ
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHCCC
QG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA