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The map label for this gene is traD [H]

Identifier: 189183022

GI number: 189183022

Start: 111173

End: 112264

Strand: Direct

Name: traD [H]

Synonym: OTT_0115

Alternate gene names: 189183022

Gene position: 111173-112264 (Clockwise)

Preceding gene: 189183021

Following gene: 189183027

Centisome position: 5.53

GC content: 32.97

Gene sequence:

>1092_bases
TTGCATGCCTTTTATTCTCTGCTTTTCATATTTTCTCTTATCCTAATCTGGCGTTCTAAATGTGATAAGCTGCTTAATCC
TTTTGAAAAAAATAGTGAACAATGGTTGCCTTGGAATGATTGTTTTGAAGCAGCTGATTTTCATGATATAGCTAGTAGTT
TTAGTAATTATACTCATAAAATTGATGACTTTTTTACTAAAAATGCTGAATTAGTTTTGTCTGAAGCGTTGAAGCTATAT
CAGGACGATAAAGATATCATAAAACTAATTCATACAATCATTTACTCTGATAATAGACAATTTGCAAAAGCTTTTAGAAA
CACTGCTGTATCAGGTATTATAAGCGAAAGCGCACTTGAAACTTCTGCAGGAATTCAGTCTACGCTTGGAAAGAATATTA
CTTCGCTACAATATTTAAAGCCTGGAGGTAGTTTTAGCATAAAAGAATGGTTTAGTAATTCAAATGAAACTGGCTGGCTA
TTTATCACAGCTAACCCAAATCAAAGAGCTACTTTATGCCCACTTATTTCAGCATGGATAAGCATAGCTATCAAAGCTTT
GATGTGTAGAAATCCTAATCATGATAACAAAAACATGTGGTTTATACTTGATGAATTGCCAGCTCTACAAAAAGTTTCGT
CTTTACCAGTTGCTTTAGCTGAAAGTAGAAAGTATGGAGGCTGCTTTGTTGCTGGATTGCAGAACATTCATCAATTAGAA
GCAATATATGGGGCTGCTGAATGTGCTTCTATGCTGGATTTGTTTAATAGTAAATTTATTTTTCGAGTTAGCGATCAGGT
TACAGCTTATAAGTCAGCATTAACACTAGGTGAGCAAGAAATTATTGAAACTCAAGAGAACTTGTCATATGGATCAAATA
CTATGCGAGATGGAGTAAATATGAATAATGTTGAGCGTAAAAAGATTTTAGTTATGCCATCTGAAATTATGAACCTACCA
GACCTAACTTGTTATGTAAAGCTTGCTGGTAACTTTCCTATCACAAAACTAACTATGCAGCTACAAAACTTAAATACAGC
TTTTGTTTGTGAATATAAATTGCTCAAAAAACTTAAATTAGTGGAGTATTAA

Upstream 100 bases:

>100_bases
TATTATGAACTAATTCTTGTAGAAATATGATCAATAAATAAACCGTTATTAATTAAAACTATATTTAATATCTCACATAA
TCATAATTGTACATTATCTC

Downstream 100 bases:

>100_bases
TTCGAAAAATTAATACTCTATGTTATTTTTTAAATTAATCACTTCTTCTTTTGACAATCCAGTATTTTCAGAAATAAATT
CAACTGAAAAGCCAGCTTTT

Product: putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MHAFYSLLFIFSLILIWRSKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLY
QDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWL
FITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLE
AIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLP
DLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKLVEY

Sequences:

>Translated_363_residues
MHAFYSLLFIFSLILIWRSKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLY
QDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWL
FITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLE
AIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLP
DLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKLVEY
>Mature_363_residues
MHAFYSLLFIFSLILIWRSKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHKIDDFFTKNAELVLSEALKLY
QDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALETSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWL
FITANPNQRATLCPLISAWISIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLE
AIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVNMNNVERKKILVMPSEIMNLP
DLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKLVEY

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 41178; Mature: 41178

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAFYSLLFIFSLILIWRSKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHK
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHH
IDDFFTKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALE
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPNQRATLCPLISAWI
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHH
SIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLE
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHH
AIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVN
HHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCC
MNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKL
CCCCCCCEEEEECHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
VEY
CCC
>Mature Secondary Structure
MHAFYSLLFIFSLILIWRSKCDKLLNPFEKNSEQWLPWNDCFEAADFHDIASSFSNYTHK
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHH
IDDFFTKNAELVLSEALKLYQDDKDIIKLIHTIIYSDNRQFAKAFRNTAVSGIISESALE
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TSAGIQSTLGKNITSLQYLKPGGSFSIKEWFSNSNETGWLFITANPNQRATLCPLISAWI
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHH
SIAIKALMCRNPNHDNKNMWFILDELPALQKVSSLPVALAESRKYGGCFVAGLQNIHQLE
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHH
AIYGAAECASMLDLFNSKFIFRVSDQVTAYKSALTLGEQEIIETQENLSYGSNTMRDGVN
HHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHCCCC
MNNVERKKILVMPSEIMNLPDLTCYVKLAGNFPITKLTMQLQNLNTAFVCEYKLLKKLKL
CCCCCCCEEEEECHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
VEY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]