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The map label for this gene is trmD [H]

Identifier: 189182986

GI number: 189182986

Start: 83123

End: 83878

Strand: Direct

Name: trmD [H]

Synonym: OTT_0079

Alternate gene names: 189182986

Gene position: 83123-83878 (Clockwise)

Preceding gene: 189182985

Following gene: 189182987

Centisome position: 4.14

GC content: 29.76

Gene sequence:

>756_bases
ATGCTTAATACCAGCAGCTTTTTTCATGCTAATATACTTTCTTTATTTCCAGAAATGTTTCCTGGACCTTTGCAATATTC
ATTAGCTGGAAAAGCATTAAAGAAAAATATATGGTCATATAAAGCTATTAATATTAAAGATTTTGGTATCACAAAACACA
AAAAAGTTGATGATGAACCTTATGGTGGAGGTTCTGGTCTAGTTATGAGAGCTGATGTTCTTGCAAAAGCAATTGATTAT
GCATTAAATAACTTAAATACTAAGCCTTATCTTTATTATATGTCTCCTAGAGGGAACATTATAAATCAAAATTTGATTAA
GGAAATTATTAAGCAAAAAAATATAATTATTATCTGTGGAAGATTTGAAGGAATTGACGAGAGAGTAATAGAAAAGTATA
ATGTGTCAGAAATTAGTTTAGGAAATTTTGTTTTATCTGGAGGAGAAATAGCAGCATTAGCTTTACTTGATAGTTGTATA
AGGCTACTTCCTGGCGTTATAGCAAATCAAAATACATTACTAGAGGAATCATTTAATACAATTAATGAAGGTAATGTGAC
TTTGCTTGAATATCCGCTATATACTAGACCAGCAGTGTGGTGTAATCTTAGAGTGCCAGAAGTCTTACTTTCTGGTGATC
ATAAGAAAATAAAGGAATGGCGATTAAATCAATCTATTAAAATTACAAAACAAAGACGCCCTCATTTTATTATTGATAAT
ACATATGATAACAAAAATTCAAGGAGCACAAAATGA

Upstream 100 bases:

>100_bases
AAATGCAAGATTTAACTCAAAAAGATACCTATATATCTAAATGCTATAAGTGATAATGATATACCTTGTCACTTTTATAT
AAAAAAATAAATTACAAATA

Downstream 100 bases:

>100_bases
ATTTAATTAAGCAATTTGAACAAGAACAAATAAAAAAACTAACTCAAGGTAAAAGCATTCCTAATTTTAGACCTGGAGAT
ACTGTAAAAGTCAACTTAAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLNTSSFFHANILSLFPEMFPGPLQYSLAGKALKKNIWSYKAINIKDFGITKHKKVDDEPYGGGSGLVMRADVLAKAIDY
ALNNLNTKPYLYYMSPRGNIINQNLIKEIIKQKNIIIICGRFEGIDERVIEKYNVSEISLGNFVLSGGEIAALALLDSCI
RLLPGVIANQNTLLEESFNTINEGNVTLLEYPLYTRPAVWCNLRVPEVLLSGDHKKIKEWRLNQSIKITKQRRPHFIIDN
TYDNKNSRSTK

Sequences:

>Translated_251_residues
MLNTSSFFHANILSLFPEMFPGPLQYSLAGKALKKNIWSYKAINIKDFGITKHKKVDDEPYGGGSGLVMRADVLAKAIDY
ALNNLNTKPYLYYMSPRGNIINQNLIKEIIKQKNIIIICGRFEGIDERVIEKYNVSEISLGNFVLSGGEIAALALLDSCI
RLLPGVIANQNTLLEESFNTINEGNVTLLEYPLYTRPAVWCNLRVPEVLLSGDHKKIKEWRLNQSIKITKQRRPHFIIDN
TYDNKNSRSTK
>Mature_251_residues
MLNTSSFFHANILSLFPEMFPGPLQYSLAGKALKKNIWSYKAINIKDFGITKHKKVDDEPYGGGSGLVMRADVLAKAIDY
ALNNLNTKPYLYYMSPRGNIINQNLIKEIIKQKNIIIICGRFEGIDERVIEKYNVSEISLGNFVLSGGEIAALALLDSCI
RLLPGVIANQNTLLEESFNTINEGNVTLLEYPLYTRPAVWCNLRVPEVLLSGDHKKIKEWRLNQSIKITKQRRPHFIIDN
TYDNKNSRSTK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=238, Percent_Identity=40.7563025210084, Blast_Score=187, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28464; Mature: 28464

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTSSFFHANILSLFPEMFPGPLQYSLAGKALKKNIWSYKAINIKDFGITKHKKVDDEP
CCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCC
YGGGSGLVMRADVLAKAIDYALNNLNTKPYLYYMSPRGNIINQNLIKEIIKQKNIIIICG
CCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEEE
RFEGIDERVIEKYNVSEISLGNFVLSGGEIAALALLDSCIRLLPGVIANQNTLLEESFNT
CCCCCHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHCC
INEGNVTLLEYPLYTRPAVWCNLRVPEVLLSGDHKKIKEWRLNQSIKITKQRRPHFIIDN
CCCCCEEEEECCCCCCCEEEEECCCCHHHHCCCHHHHHHHHCCCCEEEEECCCCEEEEEC
TYDNKNSRSTK
CCCCCCCCCCC
>Mature Secondary Structure
MLNTSSFFHANILSLFPEMFPGPLQYSLAGKALKKNIWSYKAINIKDFGITKHKKVDDEP
CCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCC
YGGGSGLVMRADVLAKAIDYALNNLNTKPYLYYMSPRGNIINQNLIKEIIKQKNIIIICG
CCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEEE
RFEGIDERVIEKYNVSEISLGNFVLSGGEIAALALLDSCIRLLPGVIANQNTLLEESFNT
CCCCCHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHCC
INEGNVTLLEYPLYTRPAVWCNLRVPEVLLSGDHKKIKEWRLNQSIKITKQRRPHFIIDN
CCCCCEEEEECCCCCCCEEEEECCCCHHHHCCCHHHHHHHHCCCCEEEEECCCCEEEEEC
TYDNKNSRSTK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA