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The map label for this gene is ksgA [H]

Identifier: 189182934

GI number: 189182934

Start: 33415

End: 34236

Strand: Direct

Name: ksgA [H]

Synonym: OTT_0027

Alternate gene names: 189182934

Gene position: 33415-34236 (Clockwise)

Preceding gene: 189182933

Following gene: 189182935

Centisome position: 1.66

GC content: 32.36

Gene sequence:

>822_bases
ATGATTGAATTGCCTACAGTATCACAGCATATGAAGCTTCATAAAATTACTGCCAATAAATCTTTGGGGCAGCATTTTCT
TCTTGACTCTAATATATGTAATAAGATTGTTAGCGTTGCTCCTAACCCAATTACTGGAAAAGTTGTATTAGAAGTAGGGC
CTGGACCTGGTGGGCTAACTAGAGCAATTTTAGCACATAACCCTAAGAAGCTAATTGTTATTGAAAAAGATGCTAGTTTT
ATTGAATTGCTGCATGAAATACCTACTATGCCTTCATCTAAACTTGAGGTTATTTGTGGTGATGCTTTGAATTTTGACTT
ATCTAATATTGAGTCAAATCGCATAATTATTATTTCAAATTTACCATATAATATTGGAACTCAACTTATTGTGCAGTGGT
TACATCAAATATCATTTGTTGAATATATGATAATTATGCTTCAGGATGAAGTAGTAGAGCGTATAACATCTCCTCATTCT
AGCAAAATGTATGGTCGAATTACAGTACTAGCACAAATTGTTTCTGATGTGCGAAAGTGTTTTAAGGTTTCTTCTAGGGC
TTTTAATCCTCCACCAAAAGTAGATTCAAGTGTTATGCTAGTATCGCCTAAAAAACATCAGTTAGATAGGAATACTATAG
AGAATGTGCAAAAAATTACTAAATTAGCTTTTAGTACTAGGAGAAAAAAACTAAAAAATTGCTTAAGTCATTTGTTAAAG
TCTATAAATATTACTGATGAGATACTTAATGCGCAATTGCATCAAAGGCCTGAGCAGTTAACTCCTGAGCAATATGTTCA
GTTAAGTAGGATATTATTATAA

Upstream 100 bases:

>100_bases
AGTTAATTACTTTGCTCCAAACTGCTAAATATTCAAAGCTTTTTAGATTTTCTGTTAAACTTTGATATCTGATTGAATTT
TAGTTTCTGTTGGTTAAATT

Downstream 100 bases:

>100_bases
GAGAGTATTATTAGTGTAAAGGCACTAAATATAACAACAACAGAATAGCTAACTAAGTAAGTGATTGGAATTGTGAGTTT
TATTATTTATAGTGGAGTAG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MIELPTVSQHMKLHKITANKSLGQHFLLDSNICNKIVSVAPNPITGKVVLEVGPGPGGLTRAILAHNPKKLIVIEKDASF
IELLHEIPTMPSSKLEVICGDALNFDLSNIESNRIIIISNLPYNIGTQLIVQWLHQISFVEYMIIMLQDEVVERITSPHS
SKMYGRITVLAQIVSDVRKCFKVSSRAFNPPPKVDSSVMLVSPKKHQLDRNTIENVQKITKLAFSTRRKKLKNCLSHLLK
SINITDEILNAQLHQRPEQLTPEQYVQLSRILL

Sequences:

>Translated_273_residues
MIELPTVSQHMKLHKITANKSLGQHFLLDSNICNKIVSVAPNPITGKVVLEVGPGPGGLTRAILAHNPKKLIVIEKDASF
IELLHEIPTMPSSKLEVICGDALNFDLSNIESNRIIIISNLPYNIGTQLIVQWLHQISFVEYMIIMLQDEVVERITSPHS
SKMYGRITVLAQIVSDVRKCFKVSSRAFNPPPKVDSSVMLVSPKKHQLDRNTIENVQKITKLAFSTRRKKLKNCLSHLLK
SINITDEILNAQLHQRPEQLTPEQYVQLSRILL
>Mature_273_residues
MIELPTVSQHMKLHKITANKSLGQHFLLDSNICNKIVSVAPNPITGKVVLEVGPGPGGLTRAILAHNPKKLIVIEKDASF
IELLHEIPTMPSSKLEVICGDALNFDLSNIESNRIIIISNLPYNIGTQLIVQWLHQISFVEYMIIMLQDEVVERITSPHS
SKMYGRITVLAQIVSDVRKCFKVSSRAFNPPPKVDSSVMLVSPKKHQLDRNTIENVQKITKLAFSTRRKKLKNCLSHLLK
SINITDEILNAQLHQRPEQLTPEQYVQLSRILL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=287, Percent_Identity=35.5400696864112, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI7657198, Length=188, Percent_Identity=35.6382978723404, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=33.7164750957854, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25141369, Length=269, Percent_Identity=30.1115241635688, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25146882, Length=215, Percent_Identity=31.6279069767442, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=206, Percent_Identity=33.0097087378641, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357273, Length=293, Percent_Identity=32.0819112627986, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358017, Length=218, Percent_Identity=33.0275229357798, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30743; Mature: 30743

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELPTVSQHMKLHKITANKSLGQHFLLDSNICNKIVSVAPNPITGKVVLEVGPGPGGLT
CCCCCCHHHHHHHHHHHCCCCCCCHHEECHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
RAILAHNPKKLIVIEKDASFIELLHEIPTMPSSKLEVICGDALNFDLSNIESNRIIIISN
HHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEC
LPYNIGTQLIVQWLHQISFVEYMIIMLQDEVVERITSPHSSKMYGRITVLAQIVSDVRKC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FKVSSRAFNPPPKVDSSVMLVSPKKHQLDRNTIENVQKITKLAFSTRRKKLKNCLSHLLK
HHHHHCCCCCCCCCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SINITDEILNAQLHQRPEQLTPEQYVQLSRILL
HCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIELPTVSQHMKLHKITANKSLGQHFLLDSNICNKIVSVAPNPITGKVVLEVGPGPGGLT
CCCCCCHHHHHHHHHHHCCCCCCCHHEECHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
RAILAHNPKKLIVIEKDASFIELLHEIPTMPSSKLEVICGDALNFDLSNIESNRIIIISN
HHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEC
LPYNIGTQLIVQWLHQISFVEYMIIMLQDEVVERITSPHSSKMYGRITVLAQIVSDVRKC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FKVSSRAFNPPPKVDSSVMLVSPKKHQLDRNTIENVQKITKLAFSTRRKKLKNCLSHLLK
HHHHHCCCCCCCCCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SINITDEILNAQLHQRPEQLTPEQYVQLSRILL
HCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA